Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads

Abstract This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology...

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Autores principales:	M. J. Alvarez-Cubero, O. Santiago, C. Martínez-Labarga, B. Martínez-García, R. Marrero- Díaz, A. Rubio-Roldan, A. M. Pérez-Gutiérrez, P. Carmona-Saez, J. A. Lorente, L. J. Martinez-Gonzalez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/451532e2744f4f8c98c8ee29c70cf82b
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spelling	oai:doaj.org-article:451532e2744f4f8c98c8ee29c70cf82b2021-12-02T11:40:46ZMethodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads10.1038/s41598-018-27819-x2045-2322https://doaj.org/article/451532e2744f4f8c98c8ee29c70cf82b2018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27819-xhttps://doaj.org/toc/2045-2322Abstract This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.M. J. Alvarez-CuberoO. SantiagoC. Martínez-LabargaB. Martínez-GarcíaR. Marrero- DíazA. Rubio-RoldanA. M. Pérez-GutiérrezP. Carmona-SaezJ. A. LorenteL. J. Martinez-GonzalezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. J. Alvarez-Cubero O. Santiago C. Martínez-Labarga B. Martínez-García R. Marrero- Díaz A. Rubio-Roldan A. M. Pérez-Gutiérrez P. Carmona-Saez J. A. Lorente L. J. Martinez-Gonzalez Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
description	Abstract This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.
format	article
author	M. J. Alvarez-Cubero O. Santiago C. Martínez-Labarga B. Martínez-García R. Marrero- Díaz A. Rubio-Roldan A. M. Pérez-Gutiérrez P. Carmona-Saez J. A. Lorente L. J. Martinez-Gonzalez
author_facet	M. J. Alvarez-Cubero O. Santiago C. Martínez-Labarga B. Martínez-García R. Marrero- Díaz A. Rubio-Roldan A. M. Pérez-Gutiérrez P. Carmona-Saez J. A. Lorente L. J. Martinez-Gonzalez
author_sort	M. J. Alvarez-Cubero
title	Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
title_short	Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
title_full	Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
title_fullStr	Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
title_full_unstemmed	Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
title_sort	methodology for y chromosome capture: a complete genome sequence of y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/451532e2744f4f8c98c8ee29c70cf82b
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Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads

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