Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
Abstract This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology...
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oai:doaj.org-article:451532e2744f4f8c98c8ee29c70cf82b2021-12-02T11:40:46ZMethodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads10.1038/s41598-018-27819-x2045-2322https://doaj.org/article/451532e2744f4f8c98c8ee29c70cf82b2018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27819-xhttps://doaj.org/toc/2045-2322Abstract This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences.M. J. Alvarez-CuberoO. SantiagoC. Martínez-LabargaB. Martínez-GarcíaR. Marrero- DíazA. Rubio-RoldanA. M. Pérez-GutiérrezP. Carmona-SaezJ. A. LorenteL. J. Martinez-GonzalezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) |
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Medicine R Science Q M. J. Alvarez-Cubero O. Santiago C. Martínez-Labarga B. Martínez-García R. Marrero- Díaz A. Rubio-Roldan A. M. Pérez-Gutiérrez P. Carmona-Saez J. A. Lorente L. J. Martinez-Gonzalez Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads |
description |
Abstract This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences. |
format |
article |
author |
M. J. Alvarez-Cubero O. Santiago C. Martínez-Labarga B. Martínez-García R. Marrero- Díaz A. Rubio-Roldan A. M. Pérez-Gutiérrez P. Carmona-Saez J. A. Lorente L. J. Martinez-Gonzalez |
author_facet |
M. J. Alvarez-Cubero O. Santiago C. Martínez-Labarga B. Martínez-García R. Marrero- Díaz A. Rubio-Roldan A. M. Pérez-Gutiérrez P. Carmona-Saez J. A. Lorente L. J. Martinez-Gonzalez |
author_sort |
M. J. Alvarez-Cubero |
title |
Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads |
title_short |
Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads |
title_full |
Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads |
title_fullStr |
Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads |
title_full_unstemmed |
Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads |
title_sort |
methodology for y chromosome capture: a complete genome sequence of y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doaj.org/article/451532e2744f4f8c98c8ee29c70cf82b |
work_keys_str_mv |
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