Droplet barcoding for massively parallel single-molecule deep sequencing

The ability to accurately sequence long DNA molecules is important across biology. Here, Lan et al. report a droplet-based method that barcodes single DNA molecules, allowing the full-length molecules to be sequenced with multi-fold coverage using short-read next-generation sequencing.

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Autores principales:	Freeman Lan, John R. Haliburton, Aaron Yuan, Adam R. Abate
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4515e37d471a4e2ab6daee8b7d910e97
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spelling	oai:doaj.org-article:4515e37d471a4e2ab6daee8b7d910e972021-12-02T15:35:22ZDroplet barcoding for massively parallel single-molecule deep sequencing10.1038/ncomms117842041-1723https://doaj.org/article/4515e37d471a4e2ab6daee8b7d910e972016-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms11784https://doaj.org/toc/2041-1723The ability to accurately sequence long DNA molecules is important across biology. Here, Lan et al. report a droplet-based method that barcodes single DNA molecules, allowing the full-length molecules to be sequenced with multi-fold coverage using short-read next-generation sequencing.Freeman LanJohn R. HaliburtonAaron YuanAdam R. AbateNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Freeman Lan John R. Haliburton Aaron Yuan Adam R. Abate Droplet barcoding for massively parallel single-molecule deep sequencing
description	The ability to accurately sequence long DNA molecules is important across biology. Here, Lan et al. report a droplet-based method that barcodes single DNA molecules, allowing the full-length molecules to be sequenced with multi-fold coverage using short-read next-generation sequencing.
format	article
author	Freeman Lan John R. Haliburton Aaron Yuan Adam R. Abate
author_facet	Freeman Lan John R. Haliburton Aaron Yuan Adam R. Abate
author_sort	Freeman Lan
title	Droplet barcoding for massively parallel single-molecule deep sequencing
title_short	Droplet barcoding for massively parallel single-molecule deep sequencing
title_full	Droplet barcoding for massively parallel single-molecule deep sequencing
title_fullStr	Droplet barcoding for massively parallel single-molecule deep sequencing
title_full_unstemmed	Droplet barcoding for massively parallel single-molecule deep sequencing
title_sort	droplet barcoding for massively parallel single-molecule deep sequencing
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/4515e37d471a4e2ab6daee8b7d910e97
work_keys_str_mv	AT freemanlan dropletbarcodingformassivelyparallelsinglemoleculedeepsequencing AT johnrhaliburton dropletbarcodingformassivelyparallelsinglemoleculedeepsequencing AT aaronyuan dropletbarcodingformassivelyparallelsinglemoleculedeepsequencing AT adamrabate dropletbarcodingformassivelyparallelsinglemoleculedeepsequencing
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Droplet barcoding for massively parallel single-molecule deep sequencing

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