Characterization and visualization of murine coagulation factor VIII-producing cells in vivo

Abstract Coagulation factors are produced from hepatocytes, whereas production of coagulation factor VIII (FVIII) from primary tissues and cell species is still controversial. Here, we tried to characterize primary FVIII-producing organ and cell species using genetically engineered mice, in which en...

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Autores principales:	Morisada Hayakawa, Asuka Sakata, Hiroko Hayakawa, Hikari Matsumoto, Takafumi Hiramoto, Yuji Kashiwakura, Nemekhbayar Baatartsogt, Noriyoshi Fukushima, Yoichi Sakata, Katsue Suzuki-Inoue, Tsukasa Ohmori
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/451a30cc356541949e0c4da28cb8db25
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spelling	oai:doaj.org-article:451a30cc356541949e0c4da28cb8db252021-12-02T16:26:29ZCharacterization and visualization of murine coagulation factor VIII-producing cells in vivo10.1038/s41598-021-94307-02045-2322https://doaj.org/article/451a30cc356541949e0c4da28cb8db252021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94307-0https://doaj.org/toc/2045-2322Abstract Coagulation factors are produced from hepatocytes, whereas production of coagulation factor VIII (FVIII) from primary tissues and cell species is still controversial. Here, we tried to characterize primary FVIII-producing organ and cell species using genetically engineered mice, in which enhanced green fluorescent protein (EGFP) was expressed instead of the F8 gene. EGFP-positive FVIII-producing cells existed only in thin sinusoidal layer of the liver and characterized as CD31high, CD146high, and lymphatic vascular endothelial hyaluronan receptor 1 (Lyve1)+. EGFP-positive cells can be clearly distinguished from lymphatic endothelial cells in the expression profile of the podoplanin− and C-type lectin-like receptor-2 (CLEC-2)+. In embryogenesis, EGFP-positive cells began to emerge at E14.5 and subsequently increased according to liver maturation. Furthermore, plasma FVIII could be abolished by crossing F8 conditional deficient mice with Lyve1-Cre mice. In conclusion, in mice, FVIII is only produced from endothelial cells exhibiting CD31high, CD146high, Lyve1+, CLEC-2+, and podoplanin− in liver sinusoidal endothelial cells.Morisada HayakawaAsuka SakataHiroko HayakawaHikari MatsumotoTakafumi HiramotoYuji KashiwakuraNemekhbayar BaatartsogtNoriyoshi FukushimaYoichi SakataKatsue Suzuki-InoueTsukasa OhmoriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Morisada Hayakawa Asuka Sakata Hiroko Hayakawa Hikari Matsumoto Takafumi Hiramoto Yuji Kashiwakura Nemekhbayar Baatartsogt Noriyoshi Fukushima Yoichi Sakata Katsue Suzuki-Inoue Tsukasa Ohmori Characterization and visualization of murine coagulation factor VIII-producing cells in vivo
description	Abstract Coagulation factors are produced from hepatocytes, whereas production of coagulation factor VIII (FVIII) from primary tissues and cell species is still controversial. Here, we tried to characterize primary FVIII-producing organ and cell species using genetically engineered mice, in which enhanced green fluorescent protein (EGFP) was expressed instead of the F8 gene. EGFP-positive FVIII-producing cells existed only in thin sinusoidal layer of the liver and characterized as CD31high, CD146high, and lymphatic vascular endothelial hyaluronan receptor 1 (Lyve1)+. EGFP-positive cells can be clearly distinguished from lymphatic endothelial cells in the expression profile of the podoplanin− and C-type lectin-like receptor-2 (CLEC-2)+. In embryogenesis, EGFP-positive cells began to emerge at E14.5 and subsequently increased according to liver maturation. Furthermore, plasma FVIII could be abolished by crossing F8 conditional deficient mice with Lyve1-Cre mice. In conclusion, in mice, FVIII is only produced from endothelial cells exhibiting CD31high, CD146high, Lyve1+, CLEC-2+, and podoplanin− in liver sinusoidal endothelial cells.
format	article
author	Morisada Hayakawa Asuka Sakata Hiroko Hayakawa Hikari Matsumoto Takafumi Hiramoto Yuji Kashiwakura Nemekhbayar Baatartsogt Noriyoshi Fukushima Yoichi Sakata Katsue Suzuki-Inoue Tsukasa Ohmori
author_facet	Morisada Hayakawa Asuka Sakata Hiroko Hayakawa Hikari Matsumoto Takafumi Hiramoto Yuji Kashiwakura Nemekhbayar Baatartsogt Noriyoshi Fukushima Yoichi Sakata Katsue Suzuki-Inoue Tsukasa Ohmori
author_sort	Morisada Hayakawa
title	Characterization and visualization of murine coagulation factor VIII-producing cells in vivo
title_short	Characterization and visualization of murine coagulation factor VIII-producing cells in vivo
title_full	Characterization and visualization of murine coagulation factor VIII-producing cells in vivo
title_fullStr	Characterization and visualization of murine coagulation factor VIII-producing cells in vivo
title_full_unstemmed	Characterization and visualization of murine coagulation factor VIII-producing cells in vivo
title_sort	characterization and visualization of murine coagulation factor viii-producing cells in vivo
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/451a30cc356541949e0c4da28cb8db25
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_version_	1718384048483598336

Characterization and visualization of murine coagulation factor VIII-producing cells in vivo

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