Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene

The von Willebrand factor binding protein in Staphylococcus lugdunensis (vWbl) comprises four major regions: the signal peptide (S), the non-repetitive (A) region, the repeat (R) region, and the wall-associated (W) region. Previous studies have demonstrated that the R region contains 10 copies of re...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lee-Chung Lin, Chun-Wen Cheng, Shih-Cheng Chang, Jang-Jih Lu
Formato: article
Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://doaj.org/article/451fe9e8c5ea4228b421627cb4dcc6d4
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:451fe9e8c5ea4228b421627cb4dcc6d4
record_format dspace
spelling oai:doaj.org-article:451fe9e8c5ea4228b421627cb4dcc6d42021-11-11T07:33:11ZMolecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene2235-298810.3389/fcimb.2021.748640https://doaj.org/article/451fe9e8c5ea4228b421627cb4dcc6d42021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.748640/fullhttps://doaj.org/toc/2235-2988The von Willebrand factor binding protein in Staphylococcus lugdunensis (vWbl) comprises four major regions: the signal peptide (S), the non-repetitive (A) region, the repeat (R) region, and the wall-associated (W) region. Previous studies have demonstrated that the R region contains 10 copies of repeating sequences; however, we reveal that the copy number of repeats in the vWbl gene varies among different S. lugdunensis isolates. In this study, an epidemiological surveillance was conducted to determine whether the copy number of repeats in vWbl in different isolates of S. lugdunensis correlates with their infectivity. The number of repeats was estimated in a total of 212 isolates, consisting of 162 isolates of oxacillin-sensitive S. lugdunensis (OSSL) and 50 isolates of oxacillin-resistant S. lugdunensis (ORSL). Our data showed that 72.5% (116/162) of OSSL isolates contained 9 (25, 15.4%), 12 (43, 26.5%), or 13 (48, 29.6%) repeats, and 90% (45/50) of ORSL isolates had 9 (32, 64%) or 13 (13, 26%) repeats. In addition, 89.6% (26 of 29) of the sequence type (ST)27 strain had 12 repeats, and 86.8% (13 of 15) of the ST4 strain had 14 repeats. Twenty-seven of the 28 isolates with nine repeats were of the staphylococcal cassette chromosome mec (SCCmec) V or Vt type and belonged to ST3, and all isolates with 13 repeats were of SCCmec II type and belonged to ST6. All isolates with nine repeats had a stop codon at the 18th codon of the third repeat, suggesting that these isolates coded for nonfunctional vWbl. Further, western blot analysis confirmed that all strains translated vWbl, and only vWbl proteins coded by genes with nine repeats were exported outside the cell. These results suggest that number of vWbl repeats in S. lugdunensis have clonal specificities and may correlate with potential pathogenicity.Lee-Chung LinChun-Wen ChengShih-Cheng ChangShih-Cheng ChangJang-Jih LuJang-Jih LuJang-Jih LuFrontiers Media S.A.articleS. lugdunensisvon Willebrand factor binding proteinpathogenesisSCCmecMLSTMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution DOAJ
collection DOAJ
language EN
topic S. lugdunensis
von Willebrand factor binding protein
pathogenesis
SCCmec
MLST
Microbiology
QR1-502
spellingShingle S. lugdunensis
von Willebrand factor binding protein
pathogenesis
SCCmec
MLST
Microbiology
QR1-502
Lee-Chung Lin
Chun-Wen Cheng
Shih-Cheng Chang
Shih-Cheng Chang
Jang-Jih Lu
Jang-Jih Lu
Jang-Jih Lu
Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene
description The von Willebrand factor binding protein in Staphylococcus lugdunensis (vWbl) comprises four major regions: the signal peptide (S), the non-repetitive (A) region, the repeat (R) region, and the wall-associated (W) region. Previous studies have demonstrated that the R region contains 10 copies of repeating sequences; however, we reveal that the copy number of repeats in the vWbl gene varies among different S. lugdunensis isolates. In this study, an epidemiological surveillance was conducted to determine whether the copy number of repeats in vWbl in different isolates of S. lugdunensis correlates with their infectivity. The number of repeats was estimated in a total of 212 isolates, consisting of 162 isolates of oxacillin-sensitive S. lugdunensis (OSSL) and 50 isolates of oxacillin-resistant S. lugdunensis (ORSL). Our data showed that 72.5% (116/162) of OSSL isolates contained 9 (25, 15.4%), 12 (43, 26.5%), or 13 (48, 29.6%) repeats, and 90% (45/50) of ORSL isolates had 9 (32, 64%) or 13 (13, 26%) repeats. In addition, 89.6% (26 of 29) of the sequence type (ST)27 strain had 12 repeats, and 86.8% (13 of 15) of the ST4 strain had 14 repeats. Twenty-seven of the 28 isolates with nine repeats were of the staphylococcal cassette chromosome mec (SCCmec) V or Vt type and belonged to ST3, and all isolates with 13 repeats were of SCCmec II type and belonged to ST6. All isolates with nine repeats had a stop codon at the 18th codon of the third repeat, suggesting that these isolates coded for nonfunctional vWbl. Further, western blot analysis confirmed that all strains translated vWbl, and only vWbl proteins coded by genes with nine repeats were exported outside the cell. These results suggest that number of vWbl repeats in S. lugdunensis have clonal specificities and may correlate with potential pathogenicity.
format article
author Lee-Chung Lin
Chun-Wen Cheng
Shih-Cheng Chang
Shih-Cheng Chang
Jang-Jih Lu
Jang-Jih Lu
Jang-Jih Lu
author_facet Lee-Chung Lin
Chun-Wen Cheng
Shih-Cheng Chang
Shih-Cheng Chang
Jang-Jih Lu
Jang-Jih Lu
Jang-Jih Lu
author_sort Lee-Chung Lin
title Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene
title_short Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene
title_full Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene
title_fullStr Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene
title_full_unstemmed Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene
title_sort molecular epidemiological survey of staphylococcus lugdunensis isolates with variable number of repeats in the von willebrand factor-binding protein gene
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/451fe9e8c5ea4228b421627cb4dcc6d4
work_keys_str_mv AT leechunglin molecularepidemiologicalsurveyofstaphylococcuslugdunensisisolateswithvariablenumberofrepeatsinthevonwillebrandfactorbindingproteingene
AT chunwencheng molecularepidemiologicalsurveyofstaphylococcuslugdunensisisolateswithvariablenumberofrepeatsinthevonwillebrandfactorbindingproteingene
AT shihchengchang molecularepidemiologicalsurveyofstaphylococcuslugdunensisisolateswithvariablenumberofrepeatsinthevonwillebrandfactorbindingproteingene
AT shihchengchang molecularepidemiologicalsurveyofstaphylococcuslugdunensisisolateswithvariablenumberofrepeatsinthevonwillebrandfactorbindingproteingene
AT jangjihlu molecularepidemiologicalsurveyofstaphylococcuslugdunensisisolateswithvariablenumberofrepeatsinthevonwillebrandfactorbindingproteingene
AT jangjihlu molecularepidemiologicalsurveyofstaphylococcuslugdunensisisolateswithvariablenumberofrepeatsinthevonwillebrandfactorbindingproteingene
AT jangjihlu molecularepidemiologicalsurveyofstaphylococcuslugdunensisisolateswithvariablenumberofrepeatsinthevonwillebrandfactorbindingproteingene
_version_ 1718439394837266432