CD73 is a major regulator of adenosinergic signalling in mouse brain.

CD73 (ecto-5'-nucleotidase) is a cell surface enzyme that regulates purinergic signalling by desphosphorylating extracellular AMP to adenosine. 5'-nucleotidases are known to be expressed in brain, but the expression of CD73 and its putative physiological functions at this location remain e...

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Autores principales:	Natalia Kulesskaya, Vootele Võikar, Marjaana Peltola, Gennady G Yegutkin, Marko Salmi, Sirpa Jalkanen, Heikki Rauvala
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4527b64461ec4b1aa2c8689470282c0f
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spelling	oai:doaj.org-article:4527b64461ec4b1aa2c8689470282c0f2021-11-18T07:42:00ZCD73 is a major regulator of adenosinergic signalling in mouse brain.1932-620310.1371/journal.pone.0066896https://doaj.org/article/4527b64461ec4b1aa2c8689470282c0f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23776700/?tool=EBIhttps://doaj.org/toc/1932-6203CD73 (ecto-5'-nucleotidase) is a cell surface enzyme that regulates purinergic signalling by desphosphorylating extracellular AMP to adenosine. 5'-nucleotidases are known to be expressed in brain, but the expression of CD73 and its putative physiological functions at this location remain elusive. Here we found, using immunohistochemistry of wild-type and CD73 deficient mice, that CD73 is prominently expressed in the basal ganglia core comprised of striatum (caudate nucleus and putamen) and globus pallidus. Furthermore, meninges and the olfactory tubercle were found to specifically express CD73. Analysis of wild type (wt) and CD73 deficient mice revealed that CD73 confers the majority of 5'-nucleotidase activity in several areas of the brain. In a battery of behavioural tests and in IntelliCage studies, the CD73 deficient mice demonstrated significantly enhanced exploratory locomotor activity, which probably reflects the prominent expression of CD73 in striatum and globus pallidus that are known to control locomotion. Furthermore, the CD73 deficient mice displayed altered social behaviour. Overall, our data provide a novel mechanistic insight into adenosinergic signalling in brain, which is implicated in the regulation of normal and pathological behaviour.Natalia KulesskayaVootele VõikarMarjaana PeltolaGennady G YegutkinMarko SalmiSirpa JalkanenHeikki RauvalaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66896 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natalia Kulesskaya Vootele Võikar Marjaana Peltola Gennady G Yegutkin Marko Salmi Sirpa Jalkanen Heikki Rauvala CD73 is a major regulator of adenosinergic signalling in mouse brain.
description	CD73 (ecto-5'-nucleotidase) is a cell surface enzyme that regulates purinergic signalling by desphosphorylating extracellular AMP to adenosine. 5'-nucleotidases are known to be expressed in brain, but the expression of CD73 and its putative physiological functions at this location remain elusive. Here we found, using immunohistochemistry of wild-type and CD73 deficient mice, that CD73 is prominently expressed in the basal ganglia core comprised of striatum (caudate nucleus and putamen) and globus pallidus. Furthermore, meninges and the olfactory tubercle were found to specifically express CD73. Analysis of wild type (wt) and CD73 deficient mice revealed that CD73 confers the majority of 5'-nucleotidase activity in several areas of the brain. In a battery of behavioural tests and in IntelliCage studies, the CD73 deficient mice demonstrated significantly enhanced exploratory locomotor activity, which probably reflects the prominent expression of CD73 in striatum and globus pallidus that are known to control locomotion. Furthermore, the CD73 deficient mice displayed altered social behaviour. Overall, our data provide a novel mechanistic insight into adenosinergic signalling in brain, which is implicated in the regulation of normal and pathological behaviour.
format	article
author	Natalia Kulesskaya Vootele Võikar Marjaana Peltola Gennady G Yegutkin Marko Salmi Sirpa Jalkanen Heikki Rauvala
author_facet	Natalia Kulesskaya Vootele Võikar Marjaana Peltola Gennady G Yegutkin Marko Salmi Sirpa Jalkanen Heikki Rauvala
author_sort	Natalia Kulesskaya
title	CD73 is a major regulator of adenosinergic signalling in mouse brain.
title_short	CD73 is a major regulator of adenosinergic signalling in mouse brain.
title_full	CD73 is a major regulator of adenosinergic signalling in mouse brain.
title_fullStr	CD73 is a major regulator of adenosinergic signalling in mouse brain.
title_full_unstemmed	CD73 is a major regulator of adenosinergic signalling in mouse brain.
title_sort	cd73 is a major regulator of adenosinergic signalling in mouse brain.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4527b64461ec4b1aa2c8689470282c0f
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CD73 is a major regulator of adenosinergic signalling in mouse brain.

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