Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC

Abstract To prepare and evaluate a new radiotracer 18F-IRS for molecular imaging mutant EGF Receptors in vitro and vivo. Uptake and efflux of 18F-IRS were performed with four NSCLC cell lines including HCC827, H1975, H358 and H520. In vivo tumor targeting and pharmacokinetics of the radiotracers wer...

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Autores principales:	Yan Song, Zunyu Xiao, Kai Wang, Xiance Wang, Chongqing Zhang, Fang Fang, Xilin Sun, Baozhong Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/452db1280f254c18bf3d8e4ea86f72c1
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spelling	oai:doaj.org-article:452db1280f254c18bf3d8e4ea86f72c12021-12-02T16:08:09ZDevelopment and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC10.1038/s41598-017-01443-72045-2322https://doaj.org/article/452db1280f254c18bf3d8e4ea86f72c12017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01443-7https://doaj.org/toc/2045-2322Abstract To prepare and evaluate a new radiotracer 18F-IRS for molecular imaging mutant EGF Receptors in vitro and vivo. Uptake and efflux of 18F-IRS were performed with four NSCLC cell lines including HCC827, H1975, H358 and H520. In vivo tumor targeting and pharmacokinetics of the radiotracers were also evaluated in HCC827, H1975, H358 and H520 tumor-bearing nude mice by PET/CT imaging. Ex vivo biodistribution assays were performed to quantify the accumulation of 18F-IRS in vivo. We also performed 18F-IRS PET/CT imaging of three patients with NSCLC. We labeled this small molecule with QD620 for flow cytometry and confocal imaging analyses. The uptakes of 18F-IRS by HCC827 and HCC827 tumors were significantly higher than those of H358, H1975 and H520, and they were reduced by the addition of 100 μM of gefitinib. Biodistribution experiments showed an accumulation of 18F-IRS in tumors of HCC827 xenografts. Flow cytometry and confocal imaging with QD620-IRS further demonstrated that binding specifically to HCC827 cells. 18F-IRS accumulation was preferential in the tumor, which was NSCLC with responsive EGFR exon 19 deleted. 18F-IRS showed high binding stability and specificity to 19 exon deleted EGFR mutation in vitro and vivo.Yan SongZunyu XiaoKai WangXiance WangChongqing ZhangFang FangXilin SunBaozhong ShenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Song Zunyu Xiao Kai Wang Xiance Wang Chongqing Zhang Fang Fang Xilin Sun Baozhong Shen Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC
description	Abstract To prepare and evaluate a new radiotracer 18F-IRS for molecular imaging mutant EGF Receptors in vitro and vivo. Uptake and efflux of 18F-IRS were performed with four NSCLC cell lines including HCC827, H1975, H358 and H520. In vivo tumor targeting and pharmacokinetics of the radiotracers were also evaluated in HCC827, H1975, H358 and H520 tumor-bearing nude mice by PET/CT imaging. Ex vivo biodistribution assays were performed to quantify the accumulation of 18F-IRS in vivo. We also performed 18F-IRS PET/CT imaging of three patients with NSCLC. We labeled this small molecule with QD620 for flow cytometry and confocal imaging analyses. The uptakes of 18F-IRS by HCC827 and HCC827 tumors were significantly higher than those of H358, H1975 and H520, and they were reduced by the addition of 100 μM of gefitinib. Biodistribution experiments showed an accumulation of 18F-IRS in tumors of HCC827 xenografts. Flow cytometry and confocal imaging with QD620-IRS further demonstrated that binding specifically to HCC827 cells. 18F-IRS accumulation was preferential in the tumor, which was NSCLC with responsive EGFR exon 19 deleted. 18F-IRS showed high binding stability and specificity to 19 exon deleted EGFR mutation in vitro and vivo.
format	article
author	Yan Song Zunyu Xiao Kai Wang Xiance Wang Chongqing Zhang Fang Fang Xilin Sun Baozhong Shen
author_facet	Yan Song Zunyu Xiao Kai Wang Xiance Wang Chongqing Zhang Fang Fang Xilin Sun Baozhong Shen
author_sort	Yan Song
title	Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC
title_short	Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC
title_full	Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC
title_fullStr	Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC
title_full_unstemmed	Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC
title_sort	development and evaluation of 18f-irs for molecular imaging mutant egf receptors in nsclc
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/452db1280f254c18bf3d8e4ea86f72c1
work_keys_str_mv	AT yansong developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc AT zunyuxiao developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc AT kaiwang developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc AT xiancewang developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc AT chongqingzhang developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc AT fangfang developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc AT xilinsun developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc AT baozhongshen developmentandevaluationof18firsformolecularimagingmutantegfreceptorsinnsclc
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Development and Evaluation of 18F-IRS for Molecular Imaging Mutant EGF Receptors in NSCLC

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