Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

Applicability of CRISPR-Cas9 for in vivo screening has so far been limited. Here the authors characterize tissue specific CRISPR in the Drosophila mushroom body, generating a library of gRNA-harboring plasmids and fly lines for in vivo screening.

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Auteurs principaux: Hagar Meltzer, Efrat Marom, Idan Alyagor, Oded Mayseless, Victoria Berkun, Netta Segal-Gilboa, Tamar Unger, David Luginbuhl, Oren Schuldiner
Format: article
Langue:EN
Publié: Nature Portfolio 2019
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Accès en ligne:https://doaj.org/article/4534af8f317e409e86de3d3b977b94e0
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Résumé:Applicability of CRISPR-Cas9 for in vivo screening has so far been limited. Here the authors characterize tissue specific CRISPR in the Drosophila mushroom body, generating a library of gRNA-harboring plasmids and fly lines for in vivo screening.