Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

Applicability of CRISPR-Cas9 for in vivo screening has so far been limited. Here the authors characterize tissue specific CRISPR in the Drosophila mushroom body, generating a library of gRNA-harboring plasmids and fly lines for in vivo screening.

Guardado en:

Detalles Bibliográficos
Autores principales:	Hagar Meltzer, Efrat Marom, Idan Alyagor, Oded Mayseless, Victoria Berkun, Netta Segal-Gilboa, Tamar Unger, David Luginbuhl, Oren Schuldiner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4534af8f317e409e86de3d3b977b94e0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4534af8f317e409e86de3d3b977b94e0
record_format	dspace
spelling	oai:doaj.org-article:4534af8f317e409e86de3d3b977b94e02021-12-02T14:39:35ZTissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila10.1038/s41467-019-10140-02041-1723https://doaj.org/article/4534af8f317e409e86de3d3b977b94e02019-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10140-0https://doaj.org/toc/2041-1723Applicability of CRISPR-Cas9 for in vivo screening has so far been limited. Here the authors characterize tissue specific CRISPR in the Drosophila mushroom body, generating a library of gRNA-harboring plasmids and fly lines for in vivo screening.Hagar MeltzerEfrat MaromIdan AlyagorOded MayselessVictoria BerkunNetta Segal-GilboaTamar UngerDavid LuginbuhlOren SchuldinerNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hagar Meltzer Efrat Marom Idan Alyagor Oded Mayseless Victoria Berkun Netta Segal-Gilboa Tamar Unger David Luginbuhl Oren Schuldiner Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
description	Applicability of CRISPR-Cas9 for in vivo screening has so far been limited. Here the authors characterize tissue specific CRISPR in the Drosophila mushroom body, generating a library of gRNA-harboring plasmids and fly lines for in vivo screening.
format	article
author	Hagar Meltzer Efrat Marom Idan Alyagor Oded Mayseless Victoria Berkun Netta Segal-Gilboa Tamar Unger David Luginbuhl Oren Schuldiner
author_facet	Hagar Meltzer Efrat Marom Idan Alyagor Oded Mayseless Victoria Berkun Netta Segal-Gilboa Tamar Unger David Luginbuhl Oren Schuldiner
author_sort	Hagar Meltzer
title	Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
title_short	Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
title_full	Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
title_fullStr	Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
title_full_unstemmed	Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
title_sort	tissue-specific (ts)crispr as an efficient strategy for in vivo screening in drosophila
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/4534af8f317e409e86de3d3b977b94e0
work_keys_str_mv	AT hagarmeltzer tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT efratmarom tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT idanalyagor tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT odedmayseless tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT victoriaberkun tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT nettasegalgilboa tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT tamarunger tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT davidluginbuhl tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila AT orenschuldiner tissuespecifictscrisprasanefficientstrategyforinvivoscreeningindrosophila
_version_	1718390587225276416

Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila

Ejemplares similares