Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila
Applicability of CRISPR-Cas9 for in vivo screening has so far been limited. Here the authors characterize tissue specific CRISPR in the Drosophila mushroom body, generating a library of gRNA-harboring plasmids and fly lines for in vivo screening.
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Main Authors: | Hagar Meltzer, Efrat Marom, Idan Alyagor, Oded Mayseless, Victoria Berkun, Netta Segal-Gilboa, Tamar Unger, David Luginbuhl, Oren Schuldiner |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2019
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Subjects: | |
Online Access: | https://doaj.org/article/4534af8f317e409e86de3d3b977b94e0 |
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