Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens

This study assessed the antimicrobial, toxicity, and phytochemical profiles of Myristica fragrans extracts. Different solvent extracts were tested for antimicrobial activity against clinical and reference microbial strains, using disc and well diffusion assays and microdilution techniques. Antioxida...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abutaha Nael, Al-Keridis Lamya Ahmed, Mohamed Rania Ali El Hadi, AL-mekhlafi Fahd A.
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	antioxidant antimicrobial gas chromatography-mass spectrometry Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/45482e52b1894eee9b4f89e5aeaed6e6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:45482e52b1894eee9b4f89e5aeaed6e6
record_format	dspace
spelling	oai:doaj.org-article:45482e52b1894eee9b4f89e5aeaed6e62021-12-05T14:10:44ZPotency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens2391-542010.1515/chem-2021-0097https://doaj.org/article/45482e52b1894eee9b4f89e5aeaed6e62021-11-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0097https://doaj.org/toc/2391-5420This study assessed the antimicrobial, toxicity, and phytochemical profiles of Myristica fragrans extracts. Different solvent extracts were tested for antimicrobial activity against clinical and reference microbial strains, using disc and well diffusion assays and microdilution techniques. Antioxidant potential was investigated using 2,2-diphenyl-1-picryhydrazyl (DPPH) assays. Cytotoxicity assay was conducted against human umbilical vein endothelial cells (HUVECs) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Acute toxicity was assessed in laboratory Swiss albino mice at a single dose of 2,000 mg/kg body weight for 14 days. To assess the phytochemical constituents, spectrophotometric and gas chromatography-mass spectrometry (GC-MS) methods were used. The chloroform extract revealed antimicrobial potencies against the Gram-positive bacteria and C. albicans with minimum inhibitory concentrations. In the DPPH assay, the IC50 value of the chloroform extract was determined to be 1.49 mg/mL. The phenolic and flavonoid contents were 26.64 ± 0.1 mg of gallic acid equivalents/g and 8.28 ± 0.1 mg quercetin equivalents/g, respectively. The IC50 value was determined to be 49 µg/mL against the HUVEC line. No mortality or morbidity was observed. GC-MS analysis indicated the presence of 2-cyclopenten-1-one (44.72%) as a major compound. The current results provide scientific support for the use of M. fragrans in folk medicine.Abutaha NaelAl-Keridis Lamya AhmedMohamed Rania Ali El HadiAL-mekhlafi Fahd A.De Gruyterarticleantioxidantantimicrobialgas chromatography-mass spectrometryChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 1096-1107 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant antimicrobial gas chromatography-mass spectrometry Chemistry QD1-999
spellingShingle	antioxidant antimicrobial gas chromatography-mass spectrometry Chemistry QD1-999 Abutaha Nael Al-Keridis Lamya Ahmed Mohamed Rania Ali El Hadi AL-mekhlafi Fahd A. Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens
description	This study assessed the antimicrobial, toxicity, and phytochemical profiles of Myristica fragrans extracts. Different solvent extracts were tested for antimicrobial activity against clinical and reference microbial strains, using disc and well diffusion assays and microdilution techniques. Antioxidant potential was investigated using 2,2-diphenyl-1-picryhydrazyl (DPPH) assays. Cytotoxicity assay was conducted against human umbilical vein endothelial cells (HUVECs) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Acute toxicity was assessed in laboratory Swiss albino mice at a single dose of 2,000 mg/kg body weight for 14 days. To assess the phytochemical constituents, spectrophotometric and gas chromatography-mass spectrometry (GC-MS) methods were used. The chloroform extract revealed antimicrobial potencies against the Gram-positive bacteria and C. albicans with minimum inhibitory concentrations. In the DPPH assay, the IC50 value of the chloroform extract was determined to be 1.49 mg/mL. The phenolic and flavonoid contents were 26.64 ± 0.1 mg of gallic acid equivalents/g and 8.28 ± 0.1 mg quercetin equivalents/g, respectively. The IC50 value was determined to be 49 µg/mL against the HUVEC line. No mortality or morbidity was observed. GC-MS analysis indicated the presence of 2-cyclopenten-1-one (44.72%) as a major compound. The current results provide scientific support for the use of M. fragrans in folk medicine.
format	article
author	Abutaha Nael Al-Keridis Lamya Ahmed Mohamed Rania Ali El Hadi AL-mekhlafi Fahd A.
author_facet	Abutaha Nael Al-Keridis Lamya Ahmed Mohamed Rania Ali El Hadi AL-mekhlafi Fahd A.
author_sort	Abutaha Nael
title	Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens
title_short	Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens
title_full	Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens
title_fullStr	Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens
title_full_unstemmed	Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens
title_sort	potency and selectivity indices of myristica fragrans houtt. mace chloroform extract against non-clinical and clinical human pathogens
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/45482e52b1894eee9b4f89e5aeaed6e6
work_keys_str_mv	AT abutahanael potencyandselectivityindicesofmyristicafragranshouttmacechloroformextractagainstnonclinicalandclinicalhumanpathogens AT alkeridislamyaahmed potencyandselectivityindicesofmyristicafragranshouttmacechloroformextractagainstnonclinicalandclinicalhumanpathogens AT mohamedraniaalielhadi potencyandselectivityindicesofmyristicafragranshouttmacechloroformextractagainstnonclinicalandclinicalhumanpathogens AT almekhlafifahda potencyandselectivityindicesofmyristicafragranshouttmacechloroformextractagainstnonclinicalandclinicalhumanpathogens
_version_	1718371784072364032

Potency and selectivity indices of Myristica fragrans Houtt. mace chloroform extract against non-clinical and clinical human pathogens

Ejemplares similares