Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach

Abstract Background Meloidae (blister beetles) are known to synthetize cantharidin (CA), a toxic and defensive terpene mainly stored in male accessory glands (MAG) and emitted outward through reflex-bleeding. Recent progresses in understanding CA biosynthesis and production organ(s) in Meloidae have...

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Autores principales:	Emiliano Fratini, Marco Salvemini, Fabrizio Lombardo, Maurizio Muzzi, Marco Molfini, Silvia Gisondi, Elia Roma, Veronica D’Ezio, Tiziana Persichini, Tecla Gasperi, Paolo Mariottini, Andrea Di Giulio, Marco Alberto Bologna, Manuela Cervelli, Emiliano Mancini
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Cantharidin Blister beetle Toxic terpene Reflex-bleeding Autohaemorrhaging Clotting Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/45494e0c09fb41a3b44e684c4063333b
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spelling	oai:doaj.org-article:45494e0c09fb41a3b44e684c4063333b2021-11-14T12:26:55ZUnraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach10.1186/s12864-021-08118-81471-2164https://doaj.org/article/45494e0c09fb41a3b44e684c4063333b2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08118-8https://doaj.org/toc/1471-2164Abstract Background Meloidae (blister beetles) are known to synthetize cantharidin (CA), a toxic and defensive terpene mainly stored in male accessory glands (MAG) and emitted outward through reflex-bleeding. Recent progresses in understanding CA biosynthesis and production organ(s) in Meloidae have been made, but the way in which self-protection is achieved from the hazardous accumulation and release of CA in blister beetles has been experimentally neglected. To provide hints on this pending question, a comparative de novo assembly transcriptomic approach was performed by targeting two tissues where CA is largely accumulated and regularly circulates in Meloidae: the male reproductive tract (MRT) and the haemolymph. Differential gene expression profiles in these tissues were examined in two blister beetle species, Lydus trimaculatus (Fabricius, 1775) (tribe Lyttini) and Mylabris variabilis (Pallas, 1781) (tribe Mylabrini). Upregulated transcripts were compared between the two species to identify conserved genes possibly involved in CA detoxification and transport. Results Based on our results, we hypothesize that, to avoid auto-intoxication, ABC, MFS or other solute transporters might sequester purported glycosylated CA precursors into MAG, and lipocalins could bind CA and mitigate its reactivity when released into the haemolymph during the autohaemorrhaging response. We also found an over-representation in haemolymph of protein-domains related to coagulation and integument repairing mechanisms that likely reflects the need to limit fluid loss during reflex-bleeding. Conclusions The de novo assembled transcriptomes of L. trimaculatus and M. variabilis here provided represent valuable genetic resources to further explore the mechanisms employed to cope with toxicity of CA in blister beetle tissues. These, if revealed, might help conceiving safe and effective drug-delivery approaches to enhance the use of CA in medicine.Emiliano FratiniMarco SalveminiFabrizio LombardoMaurizio MuzziMarco MolfiniSilvia GisondiElia RomaVeronica D’EzioTiziana PersichiniTecla GasperiPaolo MariottiniAndrea Di GiulioMarco Alberto BolognaManuela CervelliEmiliano ManciniBMCarticleCantharidinBlister beetleToxic terpeneReflex-bleedingAutohaemorrhagingClottingBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-24 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cantharidin Blister beetle Toxic terpene Reflex-bleeding Autohaemorrhaging Clotting Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Cantharidin Blister beetle Toxic terpene Reflex-bleeding Autohaemorrhaging Clotting Biotechnology TP248.13-248.65 Genetics QH426-470 Emiliano Fratini Marco Salvemini Fabrizio Lombardo Maurizio Muzzi Marco Molfini Silvia Gisondi Elia Roma Veronica D’Ezio Tiziana Persichini Tecla Gasperi Paolo Mariottini Andrea Di Giulio Marco Alberto Bologna Manuela Cervelli Emiliano Mancini Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach
description	Abstract Background Meloidae (blister beetles) are known to synthetize cantharidin (CA), a toxic and defensive terpene mainly stored in male accessory glands (MAG) and emitted outward through reflex-bleeding. Recent progresses in understanding CA biosynthesis and production organ(s) in Meloidae have been made, but the way in which self-protection is achieved from the hazardous accumulation and release of CA in blister beetles has been experimentally neglected. To provide hints on this pending question, a comparative de novo assembly transcriptomic approach was performed by targeting two tissues where CA is largely accumulated and regularly circulates in Meloidae: the male reproductive tract (MRT) and the haemolymph. Differential gene expression profiles in these tissues were examined in two blister beetle species, Lydus trimaculatus (Fabricius, 1775) (tribe Lyttini) and Mylabris variabilis (Pallas, 1781) (tribe Mylabrini). Upregulated transcripts were compared between the two species to identify conserved genes possibly involved in CA detoxification and transport. Results Based on our results, we hypothesize that, to avoid auto-intoxication, ABC, MFS or other solute transporters might sequester purported glycosylated CA precursors into MAG, and lipocalins could bind CA and mitigate its reactivity when released into the haemolymph during the autohaemorrhaging response. We also found an over-representation in haemolymph of protein-domains related to coagulation and integument repairing mechanisms that likely reflects the need to limit fluid loss during reflex-bleeding. Conclusions The de novo assembled transcriptomes of L. trimaculatus and M. variabilis here provided represent valuable genetic resources to further explore the mechanisms employed to cope with toxicity of CA in blister beetle tissues. These, if revealed, might help conceiving safe and effective drug-delivery approaches to enhance the use of CA in medicine.
format	article
author	Emiliano Fratini Marco Salvemini Fabrizio Lombardo Maurizio Muzzi Marco Molfini Silvia Gisondi Elia Roma Veronica D’Ezio Tiziana Persichini Tecla Gasperi Paolo Mariottini Andrea Di Giulio Marco Alberto Bologna Manuela Cervelli Emiliano Mancini
author_facet	Emiliano Fratini Marco Salvemini Fabrizio Lombardo Maurizio Muzzi Marco Molfini Silvia Gisondi Elia Roma Veronica D’Ezio Tiziana Persichini Tecla Gasperi Paolo Mariottini Andrea Di Giulio Marco Alberto Bologna Manuela Cervelli Emiliano Mancini
author_sort	Emiliano Fratini
title	Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach
title_short	Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach
title_full	Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach
title_fullStr	Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach
title_full_unstemmed	Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach
title_sort	unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding lydus trimaculatus and mylabris variabilis (coleoptera: meloidae): a comparative transcriptomics approach
publisher	BMC
publishDate	2021
url	https://doaj.org/article/45494e0c09fb41a3b44e684c4063333b
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Unraveling the role of male reproductive tract and haemolymph in cantharidin-exuding Lydus trimaculatus and Mylabris variabilis (Coleoptera: Meloidae): a comparative transcriptomics approach

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