Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.

In this work we analyzed the immune activation properties of different Bifidobacterium strains in order to establish their ability as inductors of specific effector (Th) or regulatory (Treg) responses. First, we determined the cytokine pattern induced by 21 Bifidobacterium strains in peripheral bloo...

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Autores principales:	Patricia López, Irene González-Rodríguez, Miguel Gueimonde, Abelardo Margolles, Ana Suárez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/455b29a2dca94b08a47fb3e8ac79106f
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spelling	oai:doaj.org-article:455b29a2dca94b08a47fb3e8ac79106f2021-11-04T06:08:03ZImmune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.1932-620310.1371/journal.pone.0024776https://doaj.org/article/455b29a2dca94b08a47fb3e8ac79106f2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21966367/?tool=EBIhttps://doaj.org/toc/1932-6203In this work we analyzed the immune activation properties of different Bifidobacterium strains in order to establish their ability as inductors of specific effector (Th) or regulatory (Treg) responses. First, we determined the cytokine pattern induced by 21 Bifidobacterium strains in peripheral blood mononuclear cells (PBMCs). Results showed that four Bifidobacterium bifidum strains showed the highest production of IL-17 as well as a poor secretion of IFNγ and TNFα, suggesting a Th17 profile whereas other Bifidobacterium strains exhibited a Th1-suggestive profile. Given the key role of Th17 subsets in mucosal defence, strains suggestive of Th17 responses and the putative Th1 Bifidobacterium breve BM12/11 were selected to stimulate dendritic cells (DC) to further determine their capability to induce the differentiation of naïve CD4(+) lymphocytes toward different Th or Treg cells. All selected strains were able to induce phenotypic DC maturation, but showed differences in cytokine stimulation, DC treated with the putative Th17 strains displaying high IL-1β/IL-12 and low IL-12/IL-10 index, whereas BM12/11-DC exhibited the highest IL-12/IL-10 ratio. Differentiation of naïve lymphocytes confirmed Th1 polarization by BM12/11. Unexpectedly, any B. bifidum strain showed significant capability for Th17 generation, and they were able to generate functional Treg, thus suggesting differences between in vivo and vitro responses. In fact, activation of memory lymphocytes present in PBMCS with these bacteria, point out the presence in vivo of specific Th17 cells, supporting the plasticity of Treg/Th17 populations and the key role of commensal bacteria in mucosal tolerance and T cell reprogramming when needed.Patricia LópezIrene González-RodríguezMiguel GueimondeAbelardo MargollesAna SuárezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24776 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patricia López Irene González-Rodríguez Miguel Gueimonde Abelardo Margolles Ana Suárez Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.
description	In this work we analyzed the immune activation properties of different Bifidobacterium strains in order to establish their ability as inductors of specific effector (Th) or regulatory (Treg) responses. First, we determined the cytokine pattern induced by 21 Bifidobacterium strains in peripheral blood mononuclear cells (PBMCs). Results showed that four Bifidobacterium bifidum strains showed the highest production of IL-17 as well as a poor secretion of IFNγ and TNFα, suggesting a Th17 profile whereas other Bifidobacterium strains exhibited a Th1-suggestive profile. Given the key role of Th17 subsets in mucosal defence, strains suggestive of Th17 responses and the putative Th1 Bifidobacterium breve BM12/11 were selected to stimulate dendritic cells (DC) to further determine their capability to induce the differentiation of naïve CD4(+) lymphocytes toward different Th or Treg cells. All selected strains were able to induce phenotypic DC maturation, but showed differences in cytokine stimulation, DC treated with the putative Th17 strains displaying high IL-1β/IL-12 and low IL-12/IL-10 index, whereas BM12/11-DC exhibited the highest IL-12/IL-10 ratio. Differentiation of naïve lymphocytes confirmed Th1 polarization by BM12/11. Unexpectedly, any B. bifidum strain showed significant capability for Th17 generation, and they were able to generate functional Treg, thus suggesting differences between in vivo and vitro responses. In fact, activation of memory lymphocytes present in PBMCS with these bacteria, point out the presence in vivo of specific Th17 cells, supporting the plasticity of Treg/Th17 populations and the key role of commensal bacteria in mucosal tolerance and T cell reprogramming when needed.
format	article
author	Patricia López Irene González-Rodríguez Miguel Gueimonde Abelardo Margolles Ana Suárez
author_facet	Patricia López Irene González-Rodríguez Miguel Gueimonde Abelardo Margolles Ana Suárez
author_sort	Patricia López
title	Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.
title_short	Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.
title_full	Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.
title_fullStr	Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.
title_full_unstemmed	Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.
title_sort	immune response to bifidobacterium bifidum strains support treg/th17 plasticity.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/455b29a2dca94b08a47fb3e8ac79106f
work_keys_str_mv	AT patricialopez immuneresponsetobifidobacteriumbifidumstrainssupporttregth17plasticity AT irenegonzalezrodriguez immuneresponsetobifidobacteriumbifidumstrainssupporttregth17plasticity AT miguelgueimonde immuneresponsetobifidobacteriumbifidumstrainssupporttregth17plasticity AT abelardomargolles immuneresponsetobifidobacteriumbifidumstrainssupporttregth17plasticity AT anasuarez immuneresponsetobifidobacteriumbifidumstrainssupporttregth17plasticity
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Immune response to Bifidobacterium bifidum strains support Treg/Th17 plasticity.

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