Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies

This study evaluates in vitro cytotoxic and antiproliferative activity on human colon tumor cell line Caco-2 (ATCC-HTB-37) of a standardized (5 mg GAE/mL) ethanolic extract from <i>Stokesia laevis</i> (Slae26), of five polyphenols compounds (reference substances, ref.), namely luteolin-7...

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Autores principales:	Georgeta Neagu, Amalia Stefaniu, Adrian Albulescu, Lucia Pintilie, Lucia Camelia Pirvu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	food bioactive compounds stokes aster ethanolic extracts health effects antiproliferative activity Caco-2 cells docking Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/455bcc5ab3df4c2fad9ae6b7ccccf541
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spelling	oai:doaj.org-article:455bcc5ab3df4c2fad9ae6b7ccccf5412021-11-11T15:02:44ZAntiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies10.3390/app112199442076-3417https://doaj.org/article/455bcc5ab3df4c2fad9ae6b7ccccf5412021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9944https://doaj.org/toc/2076-3417This study evaluates in vitro cytotoxic and antiproliferative activity on human colon tumor cell line Caco-2 (ATCC-HTB-37) of a standardized (5 mg GAE/mL) ethanolic extract from <i>Stokesia laevis</i> (Slae26), of five polyphenols compounds (reference substances, ref.), namely luteolin-7-O-glucoside, luteolin-8-C-glucoside, caffeic acid, gentisic acid, and p-aminobenzoic acid (PABA), as well as of Slae26 combinations with the five reference substances, 1:1 mass rate (GAE, ref.). Cell viability studies (MTS test) have revealed IC<sub>50</sub> values of 36 μg GAE/mL in the case of Slae26 ethanolic extract, while Slae26 combinations with the five phenolics indicated IC<sub>50</sub> values around 5 μg GAE/mL. In silico docking studies on the molecular targets human tankyrase 1 (TNKS1) and human tankyrase 2 (TNKS2) in complex with their native ligands, Co-crystallized 3J5A and Co-crystallized FLN, indicated score values of −104.15 and −76.97, respectively; in the series of the reference compounds studied, luteolin-7-O-glucoside was revealed with the best score values on both molecular targets (−80.49 and −85.17), together signifying real antiproliferative potential against human colon cancer of Slae26, of luteolin-7-O-glucoside, and of Slae26 combinations with all food-related bioactive compounds tested.Georgeta NeaguAmalia StefaniuAdrian AlbulescuLucia PintilieLucia Camelia PirvuMDPI AGarticlefood bioactive compoundsstokes aster ethanolic extractshealth effectsantiproliferative activityCaco-2 cellsdockingTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9944, p 9944 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	food bioactive compounds stokes aster ethanolic extracts health effects antiproliferative activity Caco-2 cells docking Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	food bioactive compounds stokes aster ethanolic extracts health effects antiproliferative activity Caco-2 cells docking Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Georgeta Neagu Amalia Stefaniu Adrian Albulescu Lucia Pintilie Lucia Camelia Pirvu Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies
description	This study evaluates in vitro cytotoxic and antiproliferative activity on human colon tumor cell line Caco-2 (ATCC-HTB-37) of a standardized (5 mg GAE/mL) ethanolic extract from <i>Stokesia laevis</i> (Slae26), of five polyphenols compounds (reference substances, ref.), namely luteolin-7-O-glucoside, luteolin-8-C-glucoside, caffeic acid, gentisic acid, and p-aminobenzoic acid (PABA), as well as of Slae26 combinations with the five reference substances, 1:1 mass rate (GAE, ref.). Cell viability studies (MTS test) have revealed IC<sub>50</sub> values of 36 μg GAE/mL in the case of Slae26 ethanolic extract, while Slae26 combinations with the five phenolics indicated IC<sub>50</sub> values around 5 μg GAE/mL. In silico docking studies on the molecular targets human tankyrase 1 (TNKS1) and human tankyrase 2 (TNKS2) in complex with their native ligands, Co-crystallized 3J5A and Co-crystallized FLN, indicated score values of −104.15 and −76.97, respectively; in the series of the reference compounds studied, luteolin-7-O-glucoside was revealed with the best score values on both molecular targets (−80.49 and −85.17), together signifying real antiproliferative potential against human colon cancer of Slae26, of luteolin-7-O-glucoside, and of Slae26 combinations with all food-related bioactive compounds tested.
format	article
author	Georgeta Neagu Amalia Stefaniu Adrian Albulescu Lucia Pintilie Lucia Camelia Pirvu
author_facet	Georgeta Neagu Amalia Stefaniu Adrian Albulescu Lucia Pintilie Lucia Camelia Pirvu
author_sort	Georgeta Neagu
title	Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies
title_short	Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies
title_full	Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies
title_fullStr	Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies
title_full_unstemmed	Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies
title_sort	antiproliferative activity of <i>stokesia laevis</i> ethanolic extract in combination with several food-related bioactive compounds; in vitro (caco-2) and in silico docking (tnks1 and tnks2) studies
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/455bcc5ab3df4c2fad9ae6b7ccccf541
work_keys_str_mv	AT georgetaneagu antiproliferativeactivityofistokesialaevisiethanolicextractincombinationwithseveralfoodrelatedbioactivecompoundsinvitrocaco2andinsilicodockingtnks1andtnks2studies AT amaliastefaniu antiproliferativeactivityofistokesialaevisiethanolicextractincombinationwithseveralfoodrelatedbioactivecompoundsinvitrocaco2andinsilicodockingtnks1andtnks2studies AT adrianalbulescu antiproliferativeactivityofistokesialaevisiethanolicextractincombinationwithseveralfoodrelatedbioactivecompoundsinvitrocaco2andinsilicodockingtnks1andtnks2studies AT luciapintilie antiproliferativeactivityofistokesialaevisiethanolicextractincombinationwithseveralfoodrelatedbioactivecompoundsinvitrocaco2andinsilicodockingtnks1andtnks2studies AT luciacameliapirvu antiproliferativeactivityofistokesialaevisiethanolicextractincombinationwithseveralfoodrelatedbioactivecompoundsinvitrocaco2andinsilicodockingtnks1andtnks2studies
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Antiproliferative Activity of <i>Stokesia laevis</i> Ethanolic Extract in Combination with Several Food-Related Bioactive Compounds; In Vitro (Caco-2) and In Silico Docking (TNKS1 and TNKS2) Studies

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