Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis

Abstract Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sun...

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Autores principales:	Mónica I. Fass, Máximo Rivarola, Guillermo F. Ehrenbolger, Carla A. Maringolo, Juan F. Montecchia, Facundo Quiroz, Francisco García-García, Joaquín Dopazo Blázquez, H. Esteban Hopp, Ruth A. Heinz, Norma B. Paniego, Verónica V. Lia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/455efaee4e944bdf8953449cd1edc5b0
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spelling	oai:doaj.org-article:455efaee4e944bdf8953449cd1edc5b02021-12-02T16:35:41ZExploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis10.1038/s41598-020-70315-42045-2322https://doaj.org/article/455efaee4e944bdf8953449cd1edc5b02020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-70315-4https://doaj.org/toc/2045-2322Abstract Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense.Mónica I. FassMáximo RivarolaGuillermo F. EhrenbolgerCarla A. MaringoloJuan F. MontecchiaFacundo QuirozFrancisco García-GarcíaJoaquín Dopazo BlázquezH. Esteban HoppRuth A. HeinzNorma B. PaniegoVerónica V. LiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mónica I. Fass Máximo Rivarola Guillermo F. Ehrenbolger Carla A. Maringolo Juan F. Montecchia Facundo Quiroz Francisco García-García Joaquín Dopazo Blázquez H. Esteban Hopp Ruth A. Heinz Norma B. Paniego Verónica V. Lia Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
description	Abstract Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense.
format	article
author	Mónica I. Fass Máximo Rivarola Guillermo F. Ehrenbolger Carla A. Maringolo Juan F. Montecchia Facundo Quiroz Francisco García-García Joaquín Dopazo Blázquez H. Esteban Hopp Ruth A. Heinz Norma B. Paniego Verónica V. Lia
author_facet	Mónica I. Fass Máximo Rivarola Guillermo F. Ehrenbolger Carla A. Maringolo Juan F. Montecchia Facundo Quiroz Francisco García-García Joaquín Dopazo Blázquez H. Esteban Hopp Ruth A. Heinz Norma B. Paniego Verónica V. Lia
author_sort	Mónica I. Fass
title	Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
title_short	Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
title_full	Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
title_fullStr	Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
title_full_unstemmed	Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
title_sort	exploring sunflower responses to sclerotinia head rot at early stages of infection using rna-seq analysis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/455efaee4e944bdf8953449cd1edc5b0
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Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis

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