Comparison of six commercially available STR kits for their application to touch DNA using direct PCR

With an increase in the application of direct PCR to items of forensic relevance, as well as the array of STR kits available for amplification, the need for a comprehensive investigation into the optimum STR panel for this workflow has arisen. Here we examine the relative STR amplification success o...

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Autores principales:	Belinda Martin, Duncan Taylor, Adrian Linacre
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Direct PCR STR DNA Profiling Touch DNA Human Identification Criminal law and procedure K5000-5582
Acceso en línea:	https://doaj.org/article/4560333b49ca4d5f8b603eb055d2e151
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spelling	oai:doaj.org-article:4560333b49ca4d5f8b603eb055d2e1512021-11-12T04:47:23ZComparison of six commercially available STR kits for their application to touch DNA using direct PCR2665-910710.1016/j.fsir.2021.100243https://doaj.org/article/4560333b49ca4d5f8b603eb055d2e1512021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2665910721000748https://doaj.org/toc/2665-9107With an increase in the application of direct PCR to items of forensic relevance, as well as the array of STR kits available for amplification, the need for a comprehensive investigation into the optimum STR panel for this workflow has arisen. Here we examine the relative STR amplification success of touch DNA on a range of substrates, with surface properties typical of those found in forensic investigation, using six commercially available STR kits: GlobalFiler®, Identifiler® Plus, Identifiler® Direct, VeriFiler™ Plus, Investigator® 24Plex QS, and PowerPlex® 21. We report on the percentage of possible donor alleles amplified per profile, and the number of samples that resulted in informative genetic data (≥12 autosomal alleles). We also include comment on the ease of interpretation of the resulting electropherograms for each of these six STR kits, when applied within a direct PCR workflow. Donors of known shedder status deposited DNA by handling one of five substrates (glass slide, matchstick, insulated wire, circuit board, and a plastic ziplock bag) for 15 s, 15 min post-handwashing with water. Each item was touched in triplicate by each volunteer for amplification with each STR kit resulting in a dataset of 720 samples. The difference in the number of profiles considered to be informative was found to be statistically significant when comparing STR kits (p = 0.0011) and donors (p = 2e-07), but not when considering substrates (p = 0.15). Identifiler® Plus amplification resulted in the highest profile coverage and second highest percentage of informative profiles. VeriFiler™ Plus generated informative profiles in the largest number of samples (94%) with PowerPlex® 21 amplification resulting in the fewest (79%). There was no significant difference between Investigator® 24Plex QS and GlobalFiler® in any empirical consideration; however, baseline noise and artefact presence made Investigator 24PlexQS profiles more difficult to analyse.Belinda MartinDuncan TaylorAdrian LinacreElsevierarticleDirect PCRSTR DNA ProfilingTouch DNAHuman IdentificationCriminal law and procedureK5000-5582ENForensic Science International: Reports, Vol 4, Iss , Pp 100243- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Direct PCR STR DNA Profiling Touch DNA Human Identification Criminal law and procedure K5000-5582
spellingShingle	Direct PCR STR DNA Profiling Touch DNA Human Identification Criminal law and procedure K5000-5582 Belinda Martin Duncan Taylor Adrian Linacre Comparison of six commercially available STR kits for their application to touch DNA using direct PCR
description	With an increase in the application of direct PCR to items of forensic relevance, as well as the array of STR kits available for amplification, the need for a comprehensive investigation into the optimum STR panel for this workflow has arisen. Here we examine the relative STR amplification success of touch DNA on a range of substrates, with surface properties typical of those found in forensic investigation, using six commercially available STR kits: GlobalFiler®, Identifiler® Plus, Identifiler® Direct, VeriFiler™ Plus, Investigator® 24Plex QS, and PowerPlex® 21. We report on the percentage of possible donor alleles amplified per profile, and the number of samples that resulted in informative genetic data (≥12 autosomal alleles). We also include comment on the ease of interpretation of the resulting electropherograms for each of these six STR kits, when applied within a direct PCR workflow. Donors of known shedder status deposited DNA by handling one of five substrates (glass slide, matchstick, insulated wire, circuit board, and a plastic ziplock bag) for 15 s, 15 min post-handwashing with water. Each item was touched in triplicate by each volunteer for amplification with each STR kit resulting in a dataset of 720 samples. The difference in the number of profiles considered to be informative was found to be statistically significant when comparing STR kits (p = 0.0011) and donors (p = 2e-07), but not when considering substrates (p = 0.15). Identifiler® Plus amplification resulted in the highest profile coverage and second highest percentage of informative profiles. VeriFiler™ Plus generated informative profiles in the largest number of samples (94%) with PowerPlex® 21 amplification resulting in the fewest (79%). There was no significant difference between Investigator® 24Plex QS and GlobalFiler® in any empirical consideration; however, baseline noise and artefact presence made Investigator 24PlexQS profiles more difficult to analyse.
format	article
author	Belinda Martin Duncan Taylor Adrian Linacre
author_facet	Belinda Martin Duncan Taylor Adrian Linacre
author_sort	Belinda Martin
title	Comparison of six commercially available STR kits for their application to touch DNA using direct PCR
title_short	Comparison of six commercially available STR kits for their application to touch DNA using direct PCR
title_full	Comparison of six commercially available STR kits for their application to touch DNA using direct PCR
title_fullStr	Comparison of six commercially available STR kits for their application to touch DNA using direct PCR
title_full_unstemmed	Comparison of six commercially available STR kits for their application to touch DNA using direct PCR
title_sort	comparison of six commercially available str kits for their application to touch dna using direct pcr
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4560333b49ca4d5f8b603eb055d2e151
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Comparison of six commercially available STR kits for their application to touch DNA using direct PCR

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