Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan

The aim of the research is to determine of the efficiency of developed uniplex, duplex, and multiplex variants of PCR test systems for the epizootic research of the plague natural foci. The research was carried out on the model of plague and its relative strains, and with the suspension of fleas and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	A. A. Abdirasilova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2013
Materias:	pla yope plague microbe gene cafl fleas epizooty Science Q
Acceso en línea:	https://doaj.org/article/4565a528d3f44f5dbfb788ee0fb5a550
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4565a528d3f44f5dbfb788ee0fb5a550
record_format	dspace
spelling	oai:doaj.org-article:4565a528d3f44f5dbfb788ee0fb5a5502021-11-23T06:14:29ZDiagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan2541-94202587-9596https://doaj.org/article/4565a528d3f44f5dbfb788ee0fb5a5502013-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1684https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The aim of the research is to determine of the efficiency of developed uniplex, duplex, and multiplex variants of PCR test systems for the epizootic research of the plague natural foci. The research was carried out on the model of plague and its relative strains, and with the suspension of fleas and ticks. Selection of the primers used for detection of the genes cafl, pla and yopE is carried out by the program software Primer 3 and the complex of online program BLAST® (Basic Local Alignment Search Tool. Synthesis of primers is carried out by the traditional phosphoramidite method on the automatic synthesizer DNA/RNA H6 (K&A, Germany). Purification of the received solutions of primers is carried out by the gel filtration method on Centri-Pure N10 columns (emp Biotech GmbH, Germany). Extraction of genomic DNA of Y. pestis, Y. pseudotuberculosis and Y. enterocolitica is done by QIAamp DNA Mini Kit (QIAGEN, Germany). Testing of specific primers and test system was carried out by the standard PCR. Analysis of amplification's results is conducted by standard method of horizontal agarose gel electrophoresis. In the research, Y.pestis strains from the Alive cultures museum of M. Aikimbayev's Kazakh Scientific Center for Quarantine and Zoonotic Diseases, and suspensions of ticks and fleas collected from the Central Asian Desert Plague Focus were used. For the first time in Kazakhstan, for production, PCR test systems have been developed for detection of the plague microbe in the field and laboratory conditions. The PCR test systems developed on the base of specific primers to plague microbe's genes yopE, cafl and pla helped to detect the circulation of plague microbe without Fl (without fraction). Research of suspensions of fleas and ticks collected during the plague epizooty confirmed the specificity and high sensitivity of the developed test systems.A. A. AbdirasilovaScientific Сentre for Family Health and Human Reproduction Problemsarticleplayopeplague microbegenecaflplayopefleasepizootyScienceQRUActa Biomedica Scientifica, Vol 0, Iss 6, Pp 74-81 (2013)
institution	DOAJ
collection	DOAJ
language	RU
topic	pla yope plague microbe gene cafl pla yope fleas epizooty Science Q
spellingShingle	pla yope plague microbe gene cafl pla yope fleas epizooty Science Q A. A. Abdirasilova Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan
description	The aim of the research is to determine of the efficiency of developed uniplex, duplex, and multiplex variants of PCR test systems for the epizootic research of the plague natural foci. The research was carried out on the model of plague and its relative strains, and with the suspension of fleas and ticks. Selection of the primers used for detection of the genes cafl, pla and yopE is carried out by the program software Primer 3 and the complex of online program BLAST® (Basic Local Alignment Search Tool. Synthesis of primers is carried out by the traditional phosphoramidite method on the automatic synthesizer DNA/RNA H6 (K&A, Germany). Purification of the received solutions of primers is carried out by the gel filtration method on Centri-Pure N10 columns (emp Biotech GmbH, Germany). Extraction of genomic DNA of Y. pestis, Y. pseudotuberculosis and Y. enterocolitica is done by QIAamp DNA Mini Kit (QIAGEN, Germany). Testing of specific primers and test system was carried out by the standard PCR. Analysis of amplification's results is conducted by standard method of horizontal agarose gel electrophoresis. In the research, Y.pestis strains from the Alive cultures museum of M. Aikimbayev's Kazakh Scientific Center for Quarantine and Zoonotic Diseases, and suspensions of ticks and fleas collected from the Central Asian Desert Plague Focus were used. For the first time in Kazakhstan, for production, PCR test systems have been developed for detection of the plague microbe in the field and laboratory conditions. The PCR test systems developed on the base of specific primers to plague microbe's genes yopE, cafl and pla helped to detect the circulation of plague microbe without Fl (without fraction). Research of suspensions of fleas and ticks collected during the plague epizooty confirmed the specificity and high sensitivity of the developed test systems.
format	article
author	A. A. Abdirasilova
author_facet	A. A. Abdirasilova
author_sort	A. A. Abdirasilova
title	Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan
title_short	Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan
title_full	Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan
title_fullStr	Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan
title_full_unstemmed	Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan
title_sort	diagnostic capacity of pcr test systems for epizootic study of the plague natural foci of kazakhstan
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2013
url	https://doaj.org/article/4565a528d3f44f5dbfb788ee0fb5a550
work_keys_str_mv	AT aaabdirasilova diagnosticcapacityofpcrtestsystemsforepizooticstudyoftheplaguenaturalfociofkazakhstan
_version_	1718417128078442496

Diagnostic capacity of PCR test systems for epizootic study of the plague natural foci of Kazakhstan

Ejemplares similares