EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR

Objective: To determine the effect of caffeine and vitamin D3 on the height of proliferative and hypertrophy zones of epiphyseal cartilage of developing femur of BALB/c mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: The study was conducted at anatomy d...

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Autores principales:	Maimoona Khan, Muhammad Saad Abdullah, Faiza Ambreen, Khadija Qamar
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2018
Materias:	caffeine femur hypertrophy zone proliferative zone Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4572133ed57c47eaa73cd0f918c0fda8
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spelling	oai:doaj.org-article:4572133ed57c47eaa73cd0f918c0fda82021-12-01T03:13:43ZEFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR0030-96482411-8842https://doaj.org/article/4572133ed57c47eaa73cd0f918c0fda82018-10-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/2266/1963https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To determine the effect of caffeine and vitamin D3 on the height of proliferative and hypertrophy zones of epiphyseal cartilage of developing femur of BALB/c mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: The study was conducted at anatomy department, Army Medical College (AMC), Rawalpindi, in collaboration with National Institute of Health (NIH), Islamabad from Oct 2014 to Oct 2015. Material and Methods: A total of thirty (30) BALB/c mice (15 male, 15 female), three weeks old and weighing 12-14 g, were taken and divided in to three equal groups of 10 mice each (5 male, 5 female). The control group G1 was given normal diet with water ad libitum. In addition to the same diet, animals in experimental group G2 were given 10 mg of caffeine per 100 g body weight once a day on alternate days through oral gav age for 60 days. All animals of group G3 for 60 days were given caffeine 10mg/100gm body weight on alternate day and vitamin D3 0.1μg per day by oral gav age. At completion of the experiment, effect of caffeine and vitamin D3 on the height of proliferative and hypertrophy zones of epiphyseal cartilage of mice's femur was analyzed. Results: The mean ± SD of height of proliferative and hypertrophy zones of control group G1 animals was found to be 31.5 ± 4.1μm and 43 ± 2.6μm, respectively. The mean ± SD of height of proliferative and hypertrophy zones of experimental group G2 animals was observed as 59 ± 3.2μm and 72.5 ± 7.2μm, respectively. The mean ± SD of height of proliferative and hypertrophy zones of experimental group G3 animals was measured as 46 ± 4.6μm and 54 ± 4.6μm, respectively. Conclusion: Caffeine intake altered the height of proliferative and hypertrophy zones of the epiphyseal cartilage of developing femur; however treatment with vitamin D3 ameliorated this effect.Maimoona KhanMuhammad Saad AbdullahFaiza AmbreenKhadija QamarArmy Medical College Rawalpindiarticlecaffeinefemurhypertrophy zoneproliferative zoneMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 68, Iss 5, Pp 1116-1120 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	caffeine femur hypertrophy zone proliferative zone Medicine R Medicine (General) R5-920
spellingShingle	caffeine femur hypertrophy zone proliferative zone Medicine R Medicine (General) R5-920 Maimoona Khan Muhammad Saad Abdullah Faiza Ambreen Khadija Qamar EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR
description	Objective: To determine the effect of caffeine and vitamin D3 on the height of proliferative and hypertrophy zones of epiphyseal cartilage of developing femur of BALB/c mice. Study Design: Laboratory based randomized control trial. Place and Duration of Study: The study was conducted at anatomy department, Army Medical College (AMC), Rawalpindi, in collaboration with National Institute of Health (NIH), Islamabad from Oct 2014 to Oct 2015. Material and Methods: A total of thirty (30) BALB/c mice (15 male, 15 female), three weeks old and weighing 12-14 g, were taken and divided in to three equal groups of 10 mice each (5 male, 5 female). The control group G1 was given normal diet with water ad libitum. In addition to the same diet, animals in experimental group G2 were given 10 mg of caffeine per 100 g body weight once a day on alternate days through oral gav age for 60 days. All animals of group G3 for 60 days were given caffeine 10mg/100gm body weight on alternate day and vitamin D3 0.1μg per day by oral gav age. At completion of the experiment, effect of caffeine and vitamin D3 on the height of proliferative and hypertrophy zones of epiphyseal cartilage of mice's femur was analyzed. Results: The mean ± SD of height of proliferative and hypertrophy zones of control group G1 animals was found to be 31.5 ± 4.1μm and 43 ± 2.6μm, respectively. The mean ± SD of height of proliferative and hypertrophy zones of experimental group G2 animals was observed as 59 ± 3.2μm and 72.5 ± 7.2μm, respectively. The mean ± SD of height of proliferative and hypertrophy zones of experimental group G3 animals was measured as 46 ± 4.6μm and 54 ± 4.6μm, respectively. Conclusion: Caffeine intake altered the height of proliferative and hypertrophy zones of the epiphyseal cartilage of developing femur; however treatment with vitamin D3 ameliorated this effect.
format	article
author	Maimoona Khan Muhammad Saad Abdullah Faiza Ambreen Khadija Qamar
author_facet	Maimoona Khan Muhammad Saad Abdullah Faiza Ambreen Khadija Qamar
author_sort	Maimoona Khan
title	EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR
title_short	EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR
title_full	EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR
title_fullStr	EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR
title_full_unstemmed	EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR
title_sort	effect of caffeine and vitamin d3 on proliferative and hypertrophy zones of epiphyseal cartilage of mice femur
publisher	Army Medical College Rawalpindi
publishDate	2018
url	https://doaj.org/article/4572133ed57c47eaa73cd0f918c0fda8
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EFFECT OF CAFFEINE AND VITAMIN D3 ON PROLIFERATIVE AND HYPERTROPHY ZONES OF EPIPHYSEAL CARTILAGE OF MICE FEMUR

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