Identification of genes involved in breast cancer and breast cancer stem cells

Panagiotis Apostolou, Maria Toloudi, Ioannis Papasotiriou Research and Development Department, Research Genetic Cancer Centre Ltd, Florina, Greece Abstract: Breast cancer is the most frequent type of cancer in women. Great progress has been made in its treatment but relapse is common. One hypothesi...

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Auteurs principaux:	Apostolou P, Toloudi M, Papasotiriou I
Format:	article
Langue:	EN
Publié:	Dove Medical Press 2015
Sujets:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Accès en ligne:	https://doaj.org/article/4574feded8734834810c5439db048a23
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spelling	oai:doaj.org-article:4574feded8734834810c5439db048a232021-12-02T02:19:12ZIdentification of genes involved in breast cancer and breast cancer stem cells1179-1314https://doaj.org/article/4574feded8734834810c5439db048a232015-07-01T00:00:00Zhttp://www.dovepress.com/identification-of-genes-involved-in-breast-cancer-and-breast-cancer-st-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314Panagiotis Apostolou, Maria Toloudi, Ioannis Papasotiriou Research and Development Department, Research Genetic Cancer Centre Ltd, Florina, Greece Abstract: Breast cancer is the most frequent type of cancer in women. Great progress has been made in its treatment but relapse is common. One hypothesis to account for the high recurrence rates is the presence of cancer stem cells (CSCs), which have the ability to self-renew and differentiate into multiple malignant cell types. This study aimed to determine genes that are expressed in breast cancer and breast CSCs and to investigate their correlation with stemness. RNA was extracted from established breast cancer cell lines and from CSCs derived from five different breast cancer patients. DNA microarray analysis was performed and any upregulated genes were also studied in other cancer types, including colorectal and lung cancer. For genes that were expressed only in breast cancer, knockdown-based experiments were performed. Finally, the gene expression levels of stemness transcription factors were measured. The outcome of the analysis indicated a group of genes that were aberrantly expressed mainly in breast cancer cells with stemness properties. Knockdown experiments confirmed the impact of several of these on NANOG, OCT3/4, and SOX2 transcription factors. It seems that several genes that are not directly related with hormone metabolism and basic signal transduction pathways might have an important role in relapse and disease progression and, thus, can be targeted for new treatment approaches for breast cancer. Keywords: breast cancer, cancer stem cells, stemness, DNA microarrayApostolou PToloudi MPapasotiriou IDove Medical PressarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol 2015, Iss default, Pp 183-191 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Apostolou P Toloudi M Papasotiriou I Identification of genes involved in breast cancer and breast cancer stem cells
description	Panagiotis Apostolou, Maria Toloudi, Ioannis Papasotiriou Research and Development Department, Research Genetic Cancer Centre Ltd, Florina, Greece Abstract: Breast cancer is the most frequent type of cancer in women. Great progress has been made in its treatment but relapse is common. One hypothesis to account for the high recurrence rates is the presence of cancer stem cells (CSCs), which have the ability to self-renew and differentiate into multiple malignant cell types. This study aimed to determine genes that are expressed in breast cancer and breast CSCs and to investigate their correlation with stemness. RNA was extracted from established breast cancer cell lines and from CSCs derived from five different breast cancer patients. DNA microarray analysis was performed and any upregulated genes were also studied in other cancer types, including colorectal and lung cancer. For genes that were expressed only in breast cancer, knockdown-based experiments were performed. Finally, the gene expression levels of stemness transcription factors were measured. The outcome of the analysis indicated a group of genes that were aberrantly expressed mainly in breast cancer cells with stemness properties. Knockdown experiments confirmed the impact of several of these on NANOG, OCT3/4, and SOX2 transcription factors. It seems that several genes that are not directly related with hormone metabolism and basic signal transduction pathways might have an important role in relapse and disease progression and, thus, can be targeted for new treatment approaches for breast cancer. Keywords: breast cancer, cancer stem cells, stemness, DNA microarray
format	article
author	Apostolou P Toloudi M Papasotiriou I
author_facet	Apostolou P Toloudi M Papasotiriou I
author_sort	Apostolou P
title	Identification of genes involved in breast cancer and breast cancer stem cells
title_short	Identification of genes involved in breast cancer and breast cancer stem cells
title_full	Identification of genes involved in breast cancer and breast cancer stem cells
title_fullStr	Identification of genes involved in breast cancer and breast cancer stem cells
title_full_unstemmed	Identification of genes involved in breast cancer and breast cancer stem cells
title_sort	identification of genes involved in breast cancer and breast cancer stem cells
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/4574feded8734834810c5439db048a23
work_keys_str_mv	AT apostoloup identificationofgenesinvolvedinbreastcancerandbreastcancerstemcells AT toloudim identificationofgenesinvolvedinbreastcancerandbreastcancerstemcells AT papasotirioui identificationofgenesinvolvedinbreastcancerandbreastcancerstemcells
_version_	1718402523216216064

Identification of genes involved in breast cancer and breast cancer stem cells

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