Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates

Background and objective: Pseudomonas aeruginosa is considered as a notorious pathogen due to its multidrug resistance and life threatening infections. We investigated the relationship between type III secretion toxins, biofilm formation, and antibiotic resistance among clinical P. aeruginosa isolat...

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Autores principales:	Safoura Derakhshan, Shadieh Mohammadi, Ali Rezaee
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	keywords: pseudomonas aeruginosa, biofilm, type iii secretion system, drug resistance,virulence, exoenzyme Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/45750e1780e144d6aa30855c5b323f15
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spelling	oai:doaj.org-article:45750e1780e144d6aa30855c5b323f152021-11-22T07:09:56ZRelationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates2220-76192313-7398https://doaj.org/article/45750e1780e144d6aa30855c5b323f152019-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1761https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Background and objective: Pseudomonas aeruginosa is considered as a notorious pathogen due to its multidrug resistance and life threatening infections. We investigated the relationship between type III secretion toxins, biofilm formation, and antibiotic resistance among clinical P. aeruginosa isolates.Methods: A total of 70 genetically distinct clinical P. aeruginosa isolates were characterized for antibiotic resistance by disk diffusion assay. Biofilm formation was evaluated by microtiter plate method and presence of four exo genes (exoS, exoU, exoT, and exoY) was investigated by polymerase chain reaction. A P value <0.05 was regarded statistically significant.Results: The most effective antibiotics were meropenem and piperacillin. Multidrug resistance was more prevalent in the ciprofloxacin -resistant isolates than in the - susceptible isolates. The most frequently identified exo was exoS (37.1%). Genotype exoS/exoT was found in 4 isolates, while genotype exoU/exoT was not found. Prevalence of exoS was generally higher in the susceptible isolates than in the resistant isolates. A significant association was found between the formation of strong biofilm and resistance to antibiotics (P<0.05). Prevalence of exoY and exoU was higher in the non-strong biofilm producers compared to the strong biofilm producers.Conclusion: Our study revealed formation of strong biofilm along with antibiotic resistance and the presence of exo genes in P. aeruginosa isolates. Knowledge of virulence gene profiles and biofilm formation may be useful in deciding appropriate treatment.Safoura DerakhshanShadieh MohammadiAli RezaeeSankt-Peterburg : NIIÈM imeni Pasteraarticlekeywords: pseudomonas aeruginosa, biofilm, type iii secretion system, drug resistance,virulence, exoenzymeInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 0, Iss 0 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	keywords: pseudomonas aeruginosa, biofilm, type iii secretion system, drug resistance,virulence, exoenzyme Infectious and parasitic diseases RC109-216
spellingShingle	keywords: pseudomonas aeruginosa, biofilm, type iii secretion system, drug resistance,virulence, exoenzyme Infectious and parasitic diseases RC109-216 Safoura Derakhshan Shadieh Mohammadi Ali Rezaee Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates
description	Background and objective: Pseudomonas aeruginosa is considered as a notorious pathogen due to its multidrug resistance and life threatening infections. We investigated the relationship between type III secretion toxins, biofilm formation, and antibiotic resistance among clinical P. aeruginosa isolates.Methods: A total of 70 genetically distinct clinical P. aeruginosa isolates were characterized for antibiotic resistance by disk diffusion assay. Biofilm formation was evaluated by microtiter plate method and presence of four exo genes (exoS, exoU, exoT, and exoY) was investigated by polymerase chain reaction. A P value <0.05 was regarded statistically significant.Results: The most effective antibiotics were meropenem and piperacillin. Multidrug resistance was more prevalent in the ciprofloxacin -resistant isolates than in the - susceptible isolates. The most frequently identified exo was exoS (37.1%). Genotype exoS/exoT was found in 4 isolates, while genotype exoU/exoT was not found. Prevalence of exoS was generally higher in the susceptible isolates than in the resistant isolates. A significant association was found between the formation of strong biofilm and resistance to antibiotics (P<0.05). Prevalence of exoY and exoU was higher in the non-strong biofilm producers compared to the strong biofilm producers.Conclusion: Our study revealed formation of strong biofilm along with antibiotic resistance and the presence of exo genes in P. aeruginosa isolates. Knowledge of virulence gene profiles and biofilm formation may be useful in deciding appropriate treatment.
format	article
author	Safoura Derakhshan Shadieh Mohammadi Ali Rezaee
author_facet	Safoura Derakhshan Shadieh Mohammadi Ali Rezaee
author_sort	Safoura Derakhshan
title	Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates
title_short	Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates
title_full	Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates
title_fullStr	Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates
title_full_unstemmed	Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates
title_sort	relationship between type iii secretion toxins, biofilm formation, and antibiotic resistance in clinical pseudomonas aeruginosa isolates
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/45750e1780e144d6aa30855c5b323f15
work_keys_str_mv	AT safouraderakhshan relationshipbetweentypeiiisecretiontoxinsbiofilmformationandantibioticresistanceinclinicalpseudomonasaeruginosaisolates AT shadiehmohammadi relationshipbetweentypeiiisecretiontoxinsbiofilmformationandantibioticresistanceinclinicalpseudomonasaeruginosaisolates AT alirezaee relationshipbetweentypeiiisecretiontoxinsbiofilmformationandantibioticresistanceinclinicalpseudomonasaeruginosaisolates
_version_	1718417851681865728

Relationship between type III secretion toxins, biofilm formation, and antibiotic resistance in clinical Pseudomonas aeruginosa isolates

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