Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.

The yeast γ-tubulin Tub4 is assembled with Spc97 and Spc98 into the small Tub4 complex. The Tub4 complex binds via the receptor proteins Spc72 and Spc110 to the spindle pole body (SPB), the functional equivalent of the mammalian centrosome, where the Tub4 complex organizes cytoplasmic and nuclear mi...

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Autores principales:	Tien-chen Lin, Linda Gombos, Annett Neuner, Dominik Sebastian, Jesper V Olsen, Ajla Hrle, Christian Benda, Elmar Schiebel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45788b7e5f394afd92bd54afdcf3f7ca
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spelling	oai:doaj.org-article:45788b7e5f394afd92bd54afdcf3f7ca2021-11-18T06:54:24ZPhosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.1932-620310.1371/journal.pone.0019700https://doaj.org/article/45788b7e5f394afd92bd54afdcf3f7ca2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21573187/?tool=EBIhttps://doaj.org/toc/1932-6203The yeast γ-tubulin Tub4 is assembled with Spc97 and Spc98 into the small Tub4 complex. The Tub4 complex binds via the receptor proteins Spc72 and Spc110 to the spindle pole body (SPB), the functional equivalent of the mammalian centrosome, where the Tub4 complex organizes cytoplasmic and nuclear microtubules. Little is known about the regulation of the Tub4 complex. Here, we isolated the Tub4 complex with the bound receptors from yeast cells. Analysis of the purified Tub4 complex by mass spectrometry identified more than 50 phosphorylation sites in Spc72, Spc97, Spc98, Spc110 and Tub4. To examine the functional relevance of the phosphorylation sites, phospho-mimicking and non-phosphorylatable mutations in Tub4, Spc97 and Spc98 were analyzed. Three phosphorylation sites in Tub4 were found to be critical for Tub4 stability and microtubule organization. One of the sites is highly conserved in γ-tubulins from yeast to human.Tien-chen LinLinda GombosAnnett NeunerDominik SebastianJesper V OlsenAjla HrleChristian BendaElmar SchiebelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19700 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tien-chen Lin Linda Gombos Annett Neuner Dominik Sebastian Jesper V Olsen Ajla Hrle Christian Benda Elmar Schiebel Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.
description	The yeast γ-tubulin Tub4 is assembled with Spc97 and Spc98 into the small Tub4 complex. The Tub4 complex binds via the receptor proteins Spc72 and Spc110 to the spindle pole body (SPB), the functional equivalent of the mammalian centrosome, where the Tub4 complex organizes cytoplasmic and nuclear microtubules. Little is known about the regulation of the Tub4 complex. Here, we isolated the Tub4 complex with the bound receptors from yeast cells. Analysis of the purified Tub4 complex by mass spectrometry identified more than 50 phosphorylation sites in Spc72, Spc97, Spc98, Spc110 and Tub4. To examine the functional relevance of the phosphorylation sites, phospho-mimicking and non-phosphorylatable mutations in Tub4, Spc97 and Spc98 were analyzed. Three phosphorylation sites in Tub4 were found to be critical for Tub4 stability and microtubule organization. One of the sites is highly conserved in γ-tubulins from yeast to human.
format	article
author	Tien-chen Lin Linda Gombos Annett Neuner Dominik Sebastian Jesper V Olsen Ajla Hrle Christian Benda Elmar Schiebel
author_facet	Tien-chen Lin Linda Gombos Annett Neuner Dominik Sebastian Jesper V Olsen Ajla Hrle Christian Benda Elmar Schiebel
author_sort	Tien-chen Lin
title	Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.
title_short	Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.
title_full	Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.
title_fullStr	Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.
title_full_unstemmed	Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.
title_sort	phosphorylation of the yeast γ-tubulin tub4 regulates microtubule function.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/45788b7e5f394afd92bd54afdcf3f7ca
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Phosphorylation of the yeast γ-tubulin Tub4 regulates microtubule function.

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