<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study

Currently, diagnosing type 2 diabetes (T2D) is a great challenge. Thus, there is a need to find rapid, simple, and reliable analytical methods that can detect the disease at an early stage. The aim of this work was to shed light on the importance of sample collection options, sample preparation cond...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rebeka Torok, Klaudia Horompoly, Marton Szigeti, Andras Guttman, Marta Vitai, Laszlo Koranyi, Gabor Jarvas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>N</i>-glycan type 2 diabetes capillary electrophoresis biomarker blood collecting tubes Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/4578ae237b96461ba1eca22d644918e1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4578ae237b96461ba1eca22d644918e1
record_format	dspace
spelling	oai:doaj.org-article:4578ae237b96461ba1eca22d644918e12021-11-11T18:25:19Z<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study10.3390/molecules262163991420-3049https://doaj.org/article/4578ae237b96461ba1eca22d644918e12021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6399https://doaj.org/toc/1420-3049Currently, diagnosing type 2 diabetes (T2D) is a great challenge. Thus, there is a need to find rapid, simple, and reliable analytical methods that can detect the disease at an early stage. The aim of this work was to shed light on the importance of sample collection options, sample preparation conditions, and the applied capillary electrophoresis bioanalytical technique, for a high-resolution determination of the <i>N</i>-glycan profile in human blood samples of patients with type 2 diabetes (T2D). To achieve the profile information of these complex oligosaccharides, linked by asparagine to hIgG in the blood, the glycoproteins of the samples needed to be cleaved, labelled, and purified with sufficient yield and selectivity. The resulting samples were analyzed by capillary electrophoresis, with laser-induced fluorescence detection. After separation parameter optimization, the capillary electrophoresis technique was implemented for efficient <i>N</i>-glycan profiling of whole blood samples from the diabetic patients. Our results revealed that there were subtle differences between the <i>N</i>-glycan profiles of the diabetic and control samples; in particular, two <i>N</i>-glycan structures were identified as potential glycobiomarkers that could reveal significant changes between the untreated/treated type 2 diabetic and control samples. By analyzing the resulting oligosaccharide profiles, clinically relevant information was obtained, revealing the differences between the untreated and HMG-CoA reductase-inhibitor-treated diabetic patients on changes in the <i>N</i>-glycan profile in the blood. In addition, the information from specific IgG <i>N</i>-glycosylation profiles in T2D could shed light on underlying inflammatory pathophysiological processes and lead to drug targets.Rebeka TorokKlaudia HorompolyMarton SzigetiAndras GuttmanMarta VitaiLaszlo KoranyiGabor JarvasMDPI AGarticle<i>N</i>-glycantype 2 diabetescapillary electrophoresisbiomarkerblood collecting tubesOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6399, p 6399 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>N</i>-glycan type 2 diabetes capillary electrophoresis biomarker blood collecting tubes Organic chemistry QD241-441
spellingShingle	<i>N</i>-glycan type 2 diabetes capillary electrophoresis biomarker blood collecting tubes Organic chemistry QD241-441 Rebeka Torok Klaudia Horompoly Marton Szigeti Andras Guttman Marta Vitai Laszlo Koranyi Gabor Jarvas <i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study
description	Currently, diagnosing type 2 diabetes (T2D) is a great challenge. Thus, there is a need to find rapid, simple, and reliable analytical methods that can detect the disease at an early stage. The aim of this work was to shed light on the importance of sample collection options, sample preparation conditions, and the applied capillary electrophoresis bioanalytical technique, for a high-resolution determination of the <i>N</i>-glycan profile in human blood samples of patients with type 2 diabetes (T2D). To achieve the profile information of these complex oligosaccharides, linked by asparagine to hIgG in the blood, the glycoproteins of the samples needed to be cleaved, labelled, and purified with sufficient yield and selectivity. The resulting samples were analyzed by capillary electrophoresis, with laser-induced fluorescence detection. After separation parameter optimization, the capillary electrophoresis technique was implemented for efficient <i>N</i>-glycan profiling of whole blood samples from the diabetic patients. Our results revealed that there were subtle differences between the <i>N</i>-glycan profiles of the diabetic and control samples; in particular, two <i>N</i>-glycan structures were identified as potential glycobiomarkers that could reveal significant changes between the untreated/treated type 2 diabetic and control samples. By analyzing the resulting oligosaccharide profiles, clinically relevant information was obtained, revealing the differences between the untreated and HMG-CoA reductase-inhibitor-treated diabetic patients on changes in the <i>N</i>-glycan profile in the blood. In addition, the information from specific IgG <i>N</i>-glycosylation profiles in T2D could shed light on underlying inflammatory pathophysiological processes and lead to drug targets.
format	article
author	Rebeka Torok Klaudia Horompoly Marton Szigeti Andras Guttman Marta Vitai Laszlo Koranyi Gabor Jarvas
author_facet	Rebeka Torok Klaudia Horompoly Marton Szigeti Andras Guttman Marta Vitai Laszlo Koranyi Gabor Jarvas
author_sort	Rebeka Torok
title	<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study
title_short	<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study
title_full	<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study
title_fullStr	<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study
title_full_unstemmed	<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study
title_sort	<i>n</i>-glycosylation profiling of human blood in type 2 diabetes by capillary electrophoresis: a preliminary study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4578ae237b96461ba1eca22d644918e1
work_keys_str_mv	AT rebekatorok iniglycosylationprofilingofhumanbloodintype2diabetesbycapillaryelectrophoresisapreliminarystudy AT klaudiahorompoly iniglycosylationprofilingofhumanbloodintype2diabetesbycapillaryelectrophoresisapreliminarystudy AT martonszigeti iniglycosylationprofilingofhumanbloodintype2diabetesbycapillaryelectrophoresisapreliminarystudy AT andrasguttman iniglycosylationprofilingofhumanbloodintype2diabetesbycapillaryelectrophoresisapreliminarystudy AT martavitai iniglycosylationprofilingofhumanbloodintype2diabetesbycapillaryelectrophoresisapreliminarystudy AT laszlokoranyi iniglycosylationprofilingofhumanbloodintype2diabetesbycapillaryelectrophoresisapreliminarystudy AT gaborjarvas iniglycosylationprofilingofhumanbloodintype2diabetesbycapillaryelectrophoresisapreliminarystudy
_version_	1718431820591136768

<i>N</i>-Glycosylation Profiling of Human Blood in Type 2 Diabetes by Capillary Electrophoresis: A Preliminary Study

Ejemplares similares