Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats
Neurogenesis in the adult brain is well recognized and plays a critical role in the maintenance of brain function and homeostasis. However, whether neurogenesis also occurs in the adult peripheral nervous system remains unknown. Here, using sensory ganglia (dorsal root ganglia, DRGs) as a model, we...
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2021
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oai:doaj.org-article:4580e1185d4c43cdbeb8f434e57226802021-11-14T04:29:07ZNegative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats0753-332210.1016/j.biopha.2021.112273https://doaj.org/article/4580e1185d4c43cdbeb8f434e57226802021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S075333222101057Xhttps://doaj.org/toc/0753-3322Neurogenesis in the adult brain is well recognized and plays a critical role in the maintenance of brain function and homeostasis. However, whether neurogenesis also occurs in the adult peripheral nervous system remains unknown. Here, using sensory ganglia (dorsal root ganglia, DRGs) as a model, we show that neurogenesis also occurs in the peripheral nervous system, but in a manner different from that in the central nervous system. Satellite glial cells (SGCs) express the neuronal precursor markers Nestin, POU domain, class 4, transcription factor 1, and p75 pan-neurotrophin receptor. Following sciatic nerve injury, the suppression of endogenous proBDNF by proBDNF antibodies resulted in the transformation of proliferating SGCs into doublecortin-positive cells in the DRGs. Using purified SGCs migrating out from the DRGs, the inhibition of endogenous proBDNF promoted the conversion of SGCs into neuronal phenotypes in vitro. Our findings suggest that SGCs are neuronal precursors, and that proBDNF maintains the SGC phenotype. Furthermore, the suppression of proBDNF signaling is necessary for neuronal phenotype acquisition by SGCs. Thus, we propose that peripheral neurogenesis may occur via the direct conversion of SGCs into neurons, and that this process is negatively regulated by proBDNF.Wei MaJin-Wei YangXian-Bin WangTao LuoLei ZhouAlfonso LagaresHongyun LiZhang LiangKuang-Pin LiuCheng-Hao ZangChun-Yan LiZhen WuJian-Hui GuoXin-Fu ZhouLi-Yan LiElsevierarticleSciatic nerve injuryProBDNFDRG-SGCsNeuronal precursor cellsTransdifferentiationTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112273- (2021) |
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DOAJ |
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Sciatic nerve injury ProBDNF DRG-SGCs Neuronal precursor cells Transdifferentiation Therapeutics. Pharmacology RM1-950 |
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Sciatic nerve injury ProBDNF DRG-SGCs Neuronal precursor cells Transdifferentiation Therapeutics. Pharmacology RM1-950 Wei Ma Jin-Wei Yang Xian-Bin Wang Tao Luo Lei Zhou Alfonso Lagares Hongyun Li Zhang Liang Kuang-Pin Liu Cheng-Hao Zang Chun-Yan Li Zhen Wu Jian-Hui Guo Xin-Fu Zhou Li-Yan Li Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats |
description |
Neurogenesis in the adult brain is well recognized and plays a critical role in the maintenance of brain function and homeostasis. However, whether neurogenesis also occurs in the adult peripheral nervous system remains unknown. Here, using sensory ganglia (dorsal root ganglia, DRGs) as a model, we show that neurogenesis also occurs in the peripheral nervous system, but in a manner different from that in the central nervous system. Satellite glial cells (SGCs) express the neuronal precursor markers Nestin, POU domain, class 4, transcription factor 1, and p75 pan-neurotrophin receptor. Following sciatic nerve injury, the suppression of endogenous proBDNF by proBDNF antibodies resulted in the transformation of proliferating SGCs into doublecortin-positive cells in the DRGs. Using purified SGCs migrating out from the DRGs, the inhibition of endogenous proBDNF promoted the conversion of SGCs into neuronal phenotypes in vitro. Our findings suggest that SGCs are neuronal precursors, and that proBDNF maintains the SGC phenotype. Furthermore, the suppression of proBDNF signaling is necessary for neuronal phenotype acquisition by SGCs. Thus, we propose that peripheral neurogenesis may occur via the direct conversion of SGCs into neurons, and that this process is negatively regulated by proBDNF. |
format |
article |
author |
Wei Ma Jin-Wei Yang Xian-Bin Wang Tao Luo Lei Zhou Alfonso Lagares Hongyun Li Zhang Liang Kuang-Pin Liu Cheng-Hao Zang Chun-Yan Li Zhen Wu Jian-Hui Guo Xin-Fu Zhou Li-Yan Li |
author_facet |
Wei Ma Jin-Wei Yang Xian-Bin Wang Tao Luo Lei Zhou Alfonso Lagares Hongyun Li Zhang Liang Kuang-Pin Liu Cheng-Hao Zang Chun-Yan Li Zhen Wu Jian-Hui Guo Xin-Fu Zhou Li-Yan Li |
author_sort |
Wei Ma |
title |
Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats |
title_short |
Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats |
title_full |
Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats |
title_fullStr |
Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats |
title_full_unstemmed |
Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats |
title_sort |
negative regulation by probdnf signaling of peripheral neurogenesis in the sensory ganglia of adult rats |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doaj.org/article/4580e1185d4c43cdbeb8f434e5722680 |
work_keys_str_mv |
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