Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats

Neurogenesis in the adult brain is well recognized and plays a critical role in the maintenance of brain function and homeostasis. However, whether neurogenesis also occurs in the adult peripheral nervous system remains unknown. Here, using sensory ganglia (dorsal root ganglia, DRGs) as a model, we...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Wei Ma, Jin-Wei Yang, Xian-Bin Wang, Tao Luo, Lei Zhou, Alfonso Lagares, Hongyun Li, Zhang Liang, Kuang-Pin Liu, Cheng-Hao Zang, Chun-Yan Li, Zhen Wu, Jian-Hui Guo, Xin-Fu Zhou, Li-Yan Li
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://doaj.org/article/4580e1185d4c43cdbeb8f434e5722680
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:4580e1185d4c43cdbeb8f434e5722680
record_format dspace
spelling oai:doaj.org-article:4580e1185d4c43cdbeb8f434e57226802021-11-14T04:29:07ZNegative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats0753-332210.1016/j.biopha.2021.112273https://doaj.org/article/4580e1185d4c43cdbeb8f434e57226802021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S075333222101057Xhttps://doaj.org/toc/0753-3322Neurogenesis in the adult brain is well recognized and plays a critical role in the maintenance of brain function and homeostasis. However, whether neurogenesis also occurs in the adult peripheral nervous system remains unknown. Here, using sensory ganglia (dorsal root ganglia, DRGs) as a model, we show that neurogenesis also occurs in the peripheral nervous system, but in a manner different from that in the central nervous system. Satellite glial cells (SGCs) express the neuronal precursor markers Nestin, POU domain, class 4, transcription factor 1, and p75 pan-neurotrophin receptor. Following sciatic nerve injury, the suppression of endogenous proBDNF by proBDNF antibodies resulted in the transformation of proliferating SGCs into doublecortin-positive cells in the DRGs. Using purified SGCs migrating out from the DRGs, the inhibition of endogenous proBDNF promoted the conversion of SGCs into neuronal phenotypes in vitro. Our findings suggest that SGCs are neuronal precursors, and that proBDNF maintains the SGC phenotype. Furthermore, the suppression of proBDNF signaling is necessary for neuronal phenotype acquisition by SGCs. Thus, we propose that peripheral neurogenesis may occur via the direct conversion of SGCs into neurons, and that this process is negatively regulated by proBDNF.Wei MaJin-Wei YangXian-Bin WangTao LuoLei ZhouAlfonso LagaresHongyun LiZhang LiangKuang-Pin LiuCheng-Hao ZangChun-Yan LiZhen WuJian-Hui GuoXin-Fu ZhouLi-Yan LiElsevierarticleSciatic nerve injuryProBDNFDRG-SGCsNeuronal precursor cellsTransdifferentiationTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112273- (2021)
institution DOAJ
collection DOAJ
language EN
topic Sciatic nerve injury
ProBDNF
DRG-SGCs
Neuronal precursor cells
Transdifferentiation
Therapeutics. Pharmacology
RM1-950
spellingShingle Sciatic nerve injury
ProBDNF
DRG-SGCs
Neuronal precursor cells
Transdifferentiation
Therapeutics. Pharmacology
RM1-950
Wei Ma
Jin-Wei Yang
Xian-Bin Wang
Tao Luo
Lei Zhou
Alfonso Lagares
Hongyun Li
Zhang Liang
Kuang-Pin Liu
Cheng-Hao Zang
Chun-Yan Li
Zhen Wu
Jian-Hui Guo
Xin-Fu Zhou
Li-Yan Li
Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats
description Neurogenesis in the adult brain is well recognized and plays a critical role in the maintenance of brain function and homeostasis. However, whether neurogenesis also occurs in the adult peripheral nervous system remains unknown. Here, using sensory ganglia (dorsal root ganglia, DRGs) as a model, we show that neurogenesis also occurs in the peripheral nervous system, but in a manner different from that in the central nervous system. Satellite glial cells (SGCs) express the neuronal precursor markers Nestin, POU domain, class 4, transcription factor 1, and p75 pan-neurotrophin receptor. Following sciatic nerve injury, the suppression of endogenous proBDNF by proBDNF antibodies resulted in the transformation of proliferating SGCs into doublecortin-positive cells in the DRGs. Using purified SGCs migrating out from the DRGs, the inhibition of endogenous proBDNF promoted the conversion of SGCs into neuronal phenotypes in vitro. Our findings suggest that SGCs are neuronal precursors, and that proBDNF maintains the SGC phenotype. Furthermore, the suppression of proBDNF signaling is necessary for neuronal phenotype acquisition by SGCs. Thus, we propose that peripheral neurogenesis may occur via the direct conversion of SGCs into neurons, and that this process is negatively regulated by proBDNF.
format article
author Wei Ma
Jin-Wei Yang
Xian-Bin Wang
Tao Luo
Lei Zhou
Alfonso Lagares
Hongyun Li
Zhang Liang
Kuang-Pin Liu
Cheng-Hao Zang
Chun-Yan Li
Zhen Wu
Jian-Hui Guo
Xin-Fu Zhou
Li-Yan Li
author_facet Wei Ma
Jin-Wei Yang
Xian-Bin Wang
Tao Luo
Lei Zhou
Alfonso Lagares
Hongyun Li
Zhang Liang
Kuang-Pin Liu
Cheng-Hao Zang
Chun-Yan Li
Zhen Wu
Jian-Hui Guo
Xin-Fu Zhou
Li-Yan Li
author_sort Wei Ma
title Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats
title_short Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats
title_full Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats
title_fullStr Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats
title_full_unstemmed Negative regulation by proBDNF signaling of peripheral neurogenesis in the sensory ganglia of adult rats
title_sort negative regulation by probdnf signaling of peripheral neurogenesis in the sensory ganglia of adult rats
publisher Elsevier
publishDate 2021
url https://doaj.org/article/4580e1185d4c43cdbeb8f434e5722680
work_keys_str_mv AT weima negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT jinweiyang negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT xianbinwang negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT taoluo negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT leizhou negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT alfonsolagares negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT hongyunli negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT zhangliang negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT kuangpinliu negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT chenghaozang negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT chunyanli negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT zhenwu negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT jianhuiguo negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT xinfuzhou negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
AT liyanli negativeregulationbyprobdnfsignalingofperipheralneurogenesisinthesensorygangliaofadultrats
_version_ 1718430009588187136