Production of genome-edited Daphnia for heavy metal detection by fluorescence

Abstract Aquatic heavy metal pollution is a growing concern. To facilitate heavy metal monitoring in water, we developed transgenic Daphnia that are highly sensitive to heavy metals and respond to them rapidly. Metallothionein A, which was a metal response gene, and its promoter region was obtained...

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Autores principales:	Takuto Arao, Yasuhiko Kato, Quang Dang Nong, Hiroshi Yamamoto, Haruna Watanabe, Tomoaki Matsuura, Norihisa Tatarazako, Kazune Tani, Akira Okamoto, Takeru Matsumoto, Hajime Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4582b086e72940e087dff3c8bafedcea
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spelling	oai:doaj.org-article:4582b086e72940e087dff3c8bafedcea2021-12-02T15:11:50ZProduction of genome-edited Daphnia for heavy metal detection by fluorescence10.1038/s41598-020-78572-z2045-2322https://doaj.org/article/4582b086e72940e087dff3c8bafedcea2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78572-zhttps://doaj.org/toc/2045-2322Abstract Aquatic heavy metal pollution is a growing concern. To facilitate heavy metal monitoring in water, we developed transgenic Daphnia that are highly sensitive to heavy metals and respond to them rapidly. Metallothionein A, which was a metal response gene, and its promoter region was obtained from Daphnia magna. A chimeric gene fusing the promoter region with a green fluorescent protein (GFP) gene was integrated into D. magna using the TALEN technique and transgenic Daphnia named D. magna MetalloG were produced. When D. magna MetalloG was exposed to heavy metal solutions for 1 h, GFP expression was induced only in their midgut and hepatopancreas. The lowest concentrations of heavy metals that activated GFP expression were 1.2 µM Zn2+, 130 nM Cu2+, and 70 nM Cd2+. Heavy metal exposure for 24 h could lower the thresholds even further. D. magna MetalloG facilitates aqueous heavy metal detection and might enhance water quality monitoring.Takuto AraoYasuhiko KatoQuang Dang NongHiroshi YamamotoHaruna WatanabeTomoaki MatsuuraNorihisa TatarazakoKazune TaniAkira OkamotoTakeru MatsumotoHajime WatanabeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takuto Arao Yasuhiko Kato Quang Dang Nong Hiroshi Yamamoto Haruna Watanabe Tomoaki Matsuura Norihisa Tatarazako Kazune Tani Akira Okamoto Takeru Matsumoto Hajime Watanabe Production of genome-edited Daphnia for heavy metal detection by fluorescence
description	Abstract Aquatic heavy metal pollution is a growing concern. To facilitate heavy metal monitoring in water, we developed transgenic Daphnia that are highly sensitive to heavy metals and respond to them rapidly. Metallothionein A, which was a metal response gene, and its promoter region was obtained from Daphnia magna. A chimeric gene fusing the promoter region with a green fluorescent protein (GFP) gene was integrated into D. magna using the TALEN technique and transgenic Daphnia named D. magna MetalloG were produced. When D. magna MetalloG was exposed to heavy metal solutions for 1 h, GFP expression was induced only in their midgut and hepatopancreas. The lowest concentrations of heavy metals that activated GFP expression were 1.2 µM Zn2+, 130 nM Cu2+, and 70 nM Cd2+. Heavy metal exposure for 24 h could lower the thresholds even further. D. magna MetalloG facilitates aqueous heavy metal detection and might enhance water quality monitoring.
format	article
author	Takuto Arao Yasuhiko Kato Quang Dang Nong Hiroshi Yamamoto Haruna Watanabe Tomoaki Matsuura Norihisa Tatarazako Kazune Tani Akira Okamoto Takeru Matsumoto Hajime Watanabe
author_facet	Takuto Arao Yasuhiko Kato Quang Dang Nong Hiroshi Yamamoto Haruna Watanabe Tomoaki Matsuura Norihisa Tatarazako Kazune Tani Akira Okamoto Takeru Matsumoto Hajime Watanabe
author_sort	Takuto Arao
title	Production of genome-edited Daphnia for heavy metal detection by fluorescence
title_short	Production of genome-edited Daphnia for heavy metal detection by fluorescence
title_full	Production of genome-edited Daphnia for heavy metal detection by fluorescence
title_fullStr	Production of genome-edited Daphnia for heavy metal detection by fluorescence
title_full_unstemmed	Production of genome-edited Daphnia for heavy metal detection by fluorescence
title_sort	production of genome-edited daphnia for heavy metal detection by fluorescence
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4582b086e72940e087dff3c8bafedcea
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Production of genome-edited Daphnia for heavy metal detection by fluorescence

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