The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics

Methicillin-resistant strains of <i>Staphylococcus aureus</i> (MRSA) have become a global issue for healthcare systems due to their resistance to most β-lactam antibiotics, frequently accompanied by resistance to other classes of antibiotics. In this work, we analyzed the impact of combi...

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Autores principales:	Marta Woroszyło, Daria Ciecholewska-Juśko, Adam Junka, Marcin Wardach, Grzegorz Chodaczek, Bartłomiej Dudek, Karol Fijałkowski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiotics biofilm β-lactam methicillin-resistant <i>Staphylococcus aureus</i> rotating magnetic field Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/4588bbaa8e484dc2924462eeda826a77
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spelling	oai:doaj.org-article:4588bbaa8e484dc2924462eeda826a772021-11-11T17:01:49ZThe Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics10.3390/ijms2221115511422-00671661-6596https://doaj.org/article/4588bbaa8e484dc2924462eeda826a772021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11551https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Methicillin-resistant strains of <i>Staphylococcus aureus</i> (MRSA) have become a global issue for healthcare systems due to their resistance to most β-lactam antibiotics, frequently accompanied by resistance to other classes of antibiotics. In this work, we analyzed the impact of combined use of rotating magnetic field (RMF) with various classes of antibiotics (β-lactams, glycopeptides, macrolides, lincosamides, aminoglycosides, tetracyclines, and fluoroquinolones) against nine <i>S. aureus</i> strains (eight methicillin-resistant and one methicillin-sensitive). The results indicated that the application of RMF combined with antibiotics interfering with cell walls (particularly with the β-lactam antibiotics) translate into favorable changes in staphylococcal growth inhibition zones or in minimal inhibitory concentration values compared to the control settings, which were unexposed to RMF. As an example, the MIC value of cefoxitin was reduced in all MRSA strains by up to 42 times. Apart from the β-lactams, the reduced MIC values were also found for erythromycin, clindamycin, and tetracycline (three strains), ciprofloxacin (one strain), gentamicin (six strains), and teicoplanin (seven strains). The results obtained with the use of in vitro biofilm model confirm that the disturbances caused by RMF in the bacterial cell walls increase the effectiveness of the antibiotics towards MRSA. Because the clinical demand for new therapeutic options effective against MRSA is undisputable, the outcomes and conclusions drawn from the present study may be considered an important road into the application of magnetic fields to fight infections caused by methicillin-resistant staphylococci.Marta WoroszyłoDaria Ciecholewska-JuśkoAdam JunkaMarcin WardachGrzegorz ChodaczekBartłomiej DudekKarol FijałkowskiMDPI AGarticleantibioticsbiofilmβ-lactammethicillin-resistant <i>Staphylococcus aureus</i>rotating magnetic fieldBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11551, p 11551 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotics biofilm β-lactam methicillin-resistant <i>Staphylococcus aureus</i> rotating magnetic field Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	antibiotics biofilm β-lactam methicillin-resistant <i>Staphylococcus aureus</i> rotating magnetic field Biology (General) QH301-705.5 Chemistry QD1-999 Marta Woroszyło Daria Ciecholewska-Juśko Adam Junka Marcin Wardach Grzegorz Chodaczek Bartłomiej Dudek Karol Fijałkowski The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics
description	Methicillin-resistant strains of <i>Staphylococcus aureus</i> (MRSA) have become a global issue for healthcare systems due to their resistance to most β-lactam antibiotics, frequently accompanied by resistance to other classes of antibiotics. In this work, we analyzed the impact of combined use of rotating magnetic field (RMF) with various classes of antibiotics (β-lactams, glycopeptides, macrolides, lincosamides, aminoglycosides, tetracyclines, and fluoroquinolones) against nine <i>S. aureus</i> strains (eight methicillin-resistant and one methicillin-sensitive). The results indicated that the application of RMF combined with antibiotics interfering with cell walls (particularly with the β-lactam antibiotics) translate into favorable changes in staphylococcal growth inhibition zones or in minimal inhibitory concentration values compared to the control settings, which were unexposed to RMF. As an example, the MIC value of cefoxitin was reduced in all MRSA strains by up to 42 times. Apart from the β-lactams, the reduced MIC values were also found for erythromycin, clindamycin, and tetracycline (three strains), ciprofloxacin (one strain), gentamicin (six strains), and teicoplanin (seven strains). The results obtained with the use of in vitro biofilm model confirm that the disturbances caused by RMF in the bacterial cell walls increase the effectiveness of the antibiotics towards MRSA. Because the clinical demand for new therapeutic options effective against MRSA is undisputable, the outcomes and conclusions drawn from the present study may be considered an important road into the application of magnetic fields to fight infections caused by methicillin-resistant staphylococci.
format	article
author	Marta Woroszyło Daria Ciecholewska-Juśko Adam Junka Marcin Wardach Grzegorz Chodaczek Bartłomiej Dudek Karol Fijałkowski
author_facet	Marta Woroszyło Daria Ciecholewska-Juśko Adam Junka Marcin Wardach Grzegorz Chodaczek Bartłomiej Dudek Karol Fijałkowski
author_sort	Marta Woroszyło
title	The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics
title_short	The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics
title_full	The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics
title_fullStr	The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics
title_full_unstemmed	The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics
title_sort	effect of rotating magnetic field on susceptibility profile of methicillin-resistant <i>staphylococcus aureus</i> strains exposed to activity of different groups of antibiotics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4588bbaa8e484dc2924462eeda826a77
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The Effect of Rotating Magnetic Field on Susceptibility Profile of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Exposed to Activity of Different Groups of Antibiotics

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