Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools

Abstract Genetic access to small, reproducible sets of neurons is key to an understanding of the functional wiring of the brain. Here we report the generation of a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neuro...

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Autores principales:	Dominique Förster, Irene Arnold-Ammer, Eva Laurell, Alison J. Barker, António M. Fernandes, Karin Finger-Baier, Alessandro Filosa, Thomas O. Helmbrecht, Yvonne Kölsch, Enrico Kühn, Estuardo Robles, Krasimir Slanchev, Tod R. Thiele, Herwig Baier, Fumi Kubo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4592308c86074868a69462c3c2c081a2
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spelling	oai:doaj.org-article:4592308c86074868a69462c3c2c081a22021-12-02T15:05:24ZGenetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools10.1038/s41598-017-04657-x2045-2322https://doaj.org/article/4592308c86074868a69462c3c2c081a22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04657-xhttps://doaj.org/toc/2045-2322Abstract Genetic access to small, reproducible sets of neurons is key to an understanding of the functional wiring of the brain. Here we report the generation of a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neurotransmitter-synthesizing enzymes and neuropeptides, were selected according to their expression patterns in small and unique subsets of neurons from diverse brain regions. BAC recombineering, followed by Tol2 transgenesis, was used to generate driver lines that label neuronal populations in patterns that, to a large but variable extent, recapitulate the endogenous gene expression. We used image registration to characterize, compare, and digitally superimpose the labeling patterns from our newly generated transgenic lines. This analysis revealed highly restricted and mutually exclusive tissue distributions, with striking resolution of layered brain regions such as the tectum or the rhombencephalon. We further show that a combination of Gal4 and Cre transgenes allows intersectional expression of a fluorescent reporter in regions where the expression of the two drivers overlaps. Taken together, our study offers new tools for functional studies of specific neural circuits in zebrafish.Dominique FörsterIrene Arnold-AmmerEva LaurellAlison J. BarkerAntónio M. FernandesKarin Finger-BaierAlessandro FilosaThomas O. HelmbrechtYvonne KölschEnrico KühnEstuardo RoblesKrasimir SlanchevTod R. ThieleHerwig BaierFumi KuboNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dominique Förster Irene Arnold-Ammer Eva Laurell Alison J. Barker António M. Fernandes Karin Finger-Baier Alessandro Filosa Thomas O. Helmbrecht Yvonne Kölsch Enrico Kühn Estuardo Robles Krasimir Slanchev Tod R. Thiele Herwig Baier Fumi Kubo Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools
description	Abstract Genetic access to small, reproducible sets of neurons is key to an understanding of the functional wiring of the brain. Here we report the generation of a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neurotransmitter-synthesizing enzymes and neuropeptides, were selected according to their expression patterns in small and unique subsets of neurons from diverse brain regions. BAC recombineering, followed by Tol2 transgenesis, was used to generate driver lines that label neuronal populations in patterns that, to a large but variable extent, recapitulate the endogenous gene expression. We used image registration to characterize, compare, and digitally superimpose the labeling patterns from our newly generated transgenic lines. This analysis revealed highly restricted and mutually exclusive tissue distributions, with striking resolution of layered brain regions such as the tectum or the rhombencephalon. We further show that a combination of Gal4 and Cre transgenes allows intersectional expression of a fluorescent reporter in regions where the expression of the two drivers overlaps. Taken together, our study offers new tools for functional studies of specific neural circuits in zebrafish.
format	article
author	Dominique Förster Irene Arnold-Ammer Eva Laurell Alison J. Barker António M. Fernandes Karin Finger-Baier Alessandro Filosa Thomas O. Helmbrecht Yvonne Kölsch Enrico Kühn Estuardo Robles Krasimir Slanchev Tod R. Thiele Herwig Baier Fumi Kubo
author_facet	Dominique Förster Irene Arnold-Ammer Eva Laurell Alison J. Barker António M. Fernandes Karin Finger-Baier Alessandro Filosa Thomas O. Helmbrecht Yvonne Kölsch Enrico Kühn Estuardo Robles Krasimir Slanchev Tod R. Thiele Herwig Baier Fumi Kubo
author_sort	Dominique Förster
title	Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools
title_short	Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools
title_full	Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools
title_fullStr	Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools
title_full_unstemmed	Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools
title_sort	genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of bac transgenic tools
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4592308c86074868a69462c3c2c081a2
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Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools

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