Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised

Abstract Upon insemination, sperm cells are exposed to components of the female reproductive tract (FRT) fluids, such as urea and epidermal growth factor (EGF). It has been shown that both urea and EGF use EGF receptor signaling and produce reactive oxygen species (ROS) that are required at certain...

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Autores principales:	Rasoul Kowsar, Shahrzad Ronasi, Nima Sadeghi, Khaled Sadeghi, Akio Miyamoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4592629b3c244396b915aed1fcfd2028
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spelling	oai:doaj.org-article:4592629b3c244396b915aed1fcfd20282021-12-02T13:30:51ZEpidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised10.1038/s41598-021-83929-z2045-2322https://doaj.org/article/4592629b3c244396b915aed1fcfd20282021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83929-zhttps://doaj.org/toc/2045-2322Abstract Upon insemination, sperm cells are exposed to components of the female reproductive tract (FRT) fluids, such as urea and epidermal growth factor (EGF). It has been shown that both urea and EGF use EGF receptor signaling and produce reactive oxygen species (ROS) that are required at certain levels for sperm capacitation and acrosome reaction. We therefore hypothesized that during bovine sperm capacitation, a high level of urea and EGF could interfere with sperm function through overproduction of ROS. High-level urea (40 mg/dl urea is equal to 18.8 mg/dl of blood urea nitrogen) significantly increased ROS production and TUNEL-positive sperm (sperm DNA fragmentation, sDF) percentage, but decreased HOS test score, progressive motility, acrosome reaction and capacitation. The EGF reversed the negative effects of urea on all sperm parameters, with the exception of ROS production and DNA fragmentation, which were higher in urea-EGF-incubated sperm than in control-sperm. The developmental competence of oocytes inseminated with urea-EGF-incubated sperm was significantly reduced compared to the control. A close association of ROS production or sDF with 0-pronuclear and sperm non-capacitation rates was found in the network analysis. In conclusion, EGF enhanced urea-reduced sperm motility; however, it failed to reduce urea-increased sperm ROS or sDF levels and to enhance subsequent oocyte competence. The data suggests that any study to improve sperm quality should be followed by a follow-up assessment of the fertilization outcome.Rasoul KowsarShahrzad RonasiNima SadeghiKhaled SadeghiAkio MiyamotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rasoul Kowsar Shahrzad Ronasi Nima Sadeghi Khaled Sadeghi Akio Miyamoto Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised
description	Abstract Upon insemination, sperm cells are exposed to components of the female reproductive tract (FRT) fluids, such as urea and epidermal growth factor (EGF). It has been shown that both urea and EGF use EGF receptor signaling and produce reactive oxygen species (ROS) that are required at certain levels for sperm capacitation and acrosome reaction. We therefore hypothesized that during bovine sperm capacitation, a high level of urea and EGF could interfere with sperm function through overproduction of ROS. High-level urea (40 mg/dl urea is equal to 18.8 mg/dl of blood urea nitrogen) significantly increased ROS production and TUNEL-positive sperm (sperm DNA fragmentation, sDF) percentage, but decreased HOS test score, progressive motility, acrosome reaction and capacitation. The EGF reversed the negative effects of urea on all sperm parameters, with the exception of ROS production and DNA fragmentation, which were higher in urea-EGF-incubated sperm than in control-sperm. The developmental competence of oocytes inseminated with urea-EGF-incubated sperm was significantly reduced compared to the control. A close association of ROS production or sDF with 0-pronuclear and sperm non-capacitation rates was found in the network analysis. In conclusion, EGF enhanced urea-reduced sperm motility; however, it failed to reduce urea-increased sperm ROS or sDF levels and to enhance subsequent oocyte competence. The data suggests that any study to improve sperm quality should be followed by a follow-up assessment of the fertilization outcome.
format	article
author	Rasoul Kowsar Shahrzad Ronasi Nima Sadeghi Khaled Sadeghi Akio Miyamoto
author_facet	Rasoul Kowsar Shahrzad Ronasi Nima Sadeghi Khaled Sadeghi Akio Miyamoto
author_sort	Rasoul Kowsar
title	Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised
title_short	Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised
title_full	Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised
title_fullStr	Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised
title_full_unstemmed	Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised
title_sort	epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4592629b3c244396b915aed1fcfd2028
work_keys_str_mv	AT rasoulkowsar epidermalgrowthfactoralleviatesthenegativeimpactofureaonfrozenthawedbovinespermbutthesubsequentdevelopmentalcompetenceiscompromised AT shahrzadronasi epidermalgrowthfactoralleviatesthenegativeimpactofureaonfrozenthawedbovinespermbutthesubsequentdevelopmentalcompetenceiscompromised AT nimasadeghi epidermalgrowthfactoralleviatesthenegativeimpactofureaonfrozenthawedbovinespermbutthesubsequentdevelopmentalcompetenceiscompromised AT khaledsadeghi epidermalgrowthfactoralleviatesthenegativeimpactofureaonfrozenthawedbovinespermbutthesubsequentdevelopmentalcompetenceiscompromised AT akiomiyamoto epidermalgrowthfactoralleviatesthenegativeimpactofureaonfrozenthawedbovinespermbutthesubsequentdevelopmentalcompetenceiscompromised
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Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised

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