CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing

Kelil, Gallo et al. develop an antibody discovery strategy, termed CellectSeq, for targeting integral membrane proteins on native cells. This strategy uses motif-based scoring and sequencing error-filtering algorithms to identify antibodies with high diversity and specificity even at extremely low a...

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Autores principales:	Abdellali Kelil, Eugenio Gallo, Sunandan Banerjee, Jarrett J. Adams, Sachdev S. Sidhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/45934952be24446d877a084a048a5f19
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spelling	oai:doaj.org-article:45934952be24446d877a084a048a5f192021-12-02T17:15:22ZCellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing10.1038/s42003-021-02066-52399-3642https://doaj.org/article/45934952be24446d877a084a048a5f192021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02066-5https://doaj.org/toc/2399-3642Kelil, Gallo et al. develop an antibody discovery strategy, termed CellectSeq, for targeting integral membrane proteins on native cells. This strategy uses motif-based scoring and sequencing error-filtering algorithms to identify antibodies with high diversity and specificity even at extremely low abundances in NGS pools.Abdellali KelilEugenio GalloSunandan BanerjeeJarrett J. AdamsSachdev S. SidhuNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Abdellali Kelil Eugenio Gallo Sunandan Banerjee Jarrett J. Adams Sachdev S. Sidhu CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing
description	Kelil, Gallo et al. develop an antibody discovery strategy, termed CellectSeq, for targeting integral membrane proteins on native cells. This strategy uses motif-based scoring and sequencing error-filtering algorithms to identify antibodies with high diversity and specificity even at extremely low abundances in NGS pools.
format	article
author	Abdellali Kelil Eugenio Gallo Sunandan Banerjee Jarrett J. Adams Sachdev S. Sidhu
author_facet	Abdellali Kelil Eugenio Gallo Sunandan Banerjee Jarrett J. Adams Sachdev S. Sidhu
author_sort	Abdellali Kelil
title	CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing
title_short	CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing
title_full	CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing
title_fullStr	CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing
title_full_unstemmed	CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing
title_sort	cellectseq: in silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/45934952be24446d877a084a048a5f19
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CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing

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