High throughput single cell counting in droplet-based microfluidics

Abstract Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adh...

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Autores principales:	Heng Lu, Ouriel Caen, Jeremy Vrignon, Eleonora Zonta, Zakaria El Harrak, Philippe Nizard, Jean-Christophe Baret, Valérie Taly
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/459b8ebda52547779be6493c225ec157
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spelling	oai:doaj.org-article:459b8ebda52547779be6493c225ec1572021-12-02T12:32:55ZHigh throughput single cell counting in droplet-based microfluidics10.1038/s41598-017-01454-42045-2322https://doaj.org/article/459b8ebda52547779be6493c225ec1572017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01454-4https://doaj.org/toc/2045-2322Abstract Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.Heng LuOuriel CaenJeremy VrignonEleonora ZontaZakaria El HarrakPhilippe NizardJean-Christophe BaretValérie TalyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heng Lu Ouriel Caen Jeremy Vrignon Eleonora Zonta Zakaria El Harrak Philippe Nizard Jean-Christophe Baret Valérie Taly High throughput single cell counting in droplet-based microfluidics
description	Abstract Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.
format	article
author	Heng Lu Ouriel Caen Jeremy Vrignon Eleonora Zonta Zakaria El Harrak Philippe Nizard Jean-Christophe Baret Valérie Taly
author_facet	Heng Lu Ouriel Caen Jeremy Vrignon Eleonora Zonta Zakaria El Harrak Philippe Nizard Jean-Christophe Baret Valérie Taly
author_sort	Heng Lu
title	High throughput single cell counting in droplet-based microfluidics
title_short	High throughput single cell counting in droplet-based microfluidics
title_full	High throughput single cell counting in droplet-based microfluidics
title_fullStr	High throughput single cell counting in droplet-based microfluidics
title_full_unstemmed	High throughput single cell counting in droplet-based microfluidics
title_sort	high throughput single cell counting in droplet-based microfluidics
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/459b8ebda52547779be6493c225ec157
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High throughput single cell counting in droplet-based microfluidics

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