Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice

Abstract 18F-sodium fluoride (18F-NaF) is a positron emission tomography (PET) radiotracer widely used in skeletal imaging and has also been proposed as a biomarker of active calcification in atherosclerosis. Like most PET radiotracers, 18F-NaF is typically administered intravenously. However in sma...

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Autores principales:	Zaniah N. Gonzalez-Galofre, Carlos J. Alcaide-Corral, Adriana A. S. Tavares
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/459e6fe8f7044373ac50b9b594fb0b0c
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spelling	oai:doaj.org-article:459e6fe8f7044373ac50b9b594fb0b0c2021-12-02T15:54:06ZEffects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice10.1038/s41598-021-85073-02045-2322https://doaj.org/article/459e6fe8f7044373ac50b9b594fb0b0c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85073-0https://doaj.org/toc/2045-2322Abstract 18F-sodium fluoride (18F-NaF) is a positron emission tomography (PET) radiotracer widely used in skeletal imaging and has also been proposed as a biomarker of active calcification in atherosclerosis. Like most PET radiotracers, 18F-NaF is typically administered intravenously. However in small animal research intravenous administrations can be challenging, because partial paravenous injection is common due to the small calibre of the superficial tail veins and repeat administrations via tail veins can lead to tissue injury therefore limiting the total number of longitudinal scanning points. In this paper, the feasibility of using intra-peritoneal route of injection of 8F-NaF to study calcification in mice was studied by looking at the kinetic and uptake profiles of normal soft tissues and bones versus intra-vascular injections. Dynamic PET was performed for 60 min on nineteen isoflurane-anesthetized male Swiss mice after femoral artery (n = 7), femoral vein (n = 6) or intraperitoneal (n = 6) injection of 8F-NaF. PET data were reconstructed and the standardised uptake value (SUV) and standardised uptake value ratio (SUVr) were estimated from the last three frames between 45- and 60-min and 8F-NaF uptake constant (K i) was derived by Patlak graphical analysis. In soft tissue, the 18F-NaF perfusion phase changes depending on the type on injection route, whereas the uptake phase is similar regardless of the administration route. In bone tissue SUV, SUVr and K i measures were not significantly different between the three administration routes. Comparison between PET and CT measures showed that bones that had the highest CT density displayed the lowest PET activity and conversely, bones where CT units were low had high 8F-NaF uptake. Intraperitoneal injection is a valid and practical alternative to the intra-vascular injections in small-animal 18F-NaF PET imaging providing equivalent pharmacokinetic data. CT outcome measures report on sites of stablished calcification whereas PET measures sites of higher complexity and active calcification.Zaniah N. Gonzalez-GalofreCarlos J. Alcaide-CorralAdriana A. S. TavaresNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zaniah N. Gonzalez-Galofre Carlos J. Alcaide-Corral Adriana A. S. Tavares Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
description	Abstract 18F-sodium fluoride (18F-NaF) is a positron emission tomography (PET) radiotracer widely used in skeletal imaging and has also been proposed as a biomarker of active calcification in atherosclerosis. Like most PET radiotracers, 18F-NaF is typically administered intravenously. However in small animal research intravenous administrations can be challenging, because partial paravenous injection is common due to the small calibre of the superficial tail veins and repeat administrations via tail veins can lead to tissue injury therefore limiting the total number of longitudinal scanning points. In this paper, the feasibility of using intra-peritoneal route of injection of 8F-NaF to study calcification in mice was studied by looking at the kinetic and uptake profiles of normal soft tissues and bones versus intra-vascular injections. Dynamic PET was performed for 60 min on nineteen isoflurane-anesthetized male Swiss mice after femoral artery (n = 7), femoral vein (n = 6) or intraperitoneal (n = 6) injection of 8F-NaF. PET data were reconstructed and the standardised uptake value (SUV) and standardised uptake value ratio (SUVr) were estimated from the last three frames between 45- and 60-min and 8F-NaF uptake constant (K i) was derived by Patlak graphical analysis. In soft tissue, the 18F-NaF perfusion phase changes depending on the type on injection route, whereas the uptake phase is similar regardless of the administration route. In bone tissue SUV, SUVr and K i measures were not significantly different between the three administration routes. Comparison between PET and CT measures showed that bones that had the highest CT density displayed the lowest PET activity and conversely, bones where CT units were low had high 8F-NaF uptake. Intraperitoneal injection is a valid and practical alternative to the intra-vascular injections in small-animal 18F-NaF PET imaging providing equivalent pharmacokinetic data. CT outcome measures report on sites of stablished calcification whereas PET measures sites of higher complexity and active calcification.
format	article
author	Zaniah N. Gonzalez-Galofre Carlos J. Alcaide-Corral Adriana A. S. Tavares
author_facet	Zaniah N. Gonzalez-Galofre Carlos J. Alcaide-Corral Adriana A. S. Tavares
author_sort	Zaniah N. Gonzalez-Galofre
title	Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
title_short	Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
title_full	Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
title_fullStr	Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
title_full_unstemmed	Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
title_sort	effects of administration route on uptake kinetics of 18f-sodium fluoride positron emission tomography in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/459e6fe8f7044373ac50b9b594fb0b0c
work_keys_str_mv	AT zaniahngonzalezgalofre effectsofadministrationrouteonuptakekineticsof18fsodiumfluoridepositronemissiontomographyinmice AT carlosjalcaidecorral effectsofadministrationrouteonuptakekineticsof18fsodiumfluoridepositronemissiontomographyinmice AT adrianaastavares effectsofadministrationrouteonuptakekineticsof18fsodiumfluoridepositronemissiontomographyinmice
_version_	1718385444245209088

Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice

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