Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles

ABSTRACT Soil microbial community assembly is crucial for understanding the mechanisms of microbial communities that regulate ecosystem-level functioning. The relative contributions of stochastic and deterministic processes to microbial community assembly remain poorly defined, and major questions e...

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Autores principales:	Lu Luan, Chao Liang, Lijun Chen, Haotian Wang, Qinsong Xu, Yuji Jiang, Bo Sun
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	bacterial community assembly microbial metabolism bacterial interactions migration rate environmental filtering soil profile Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/45a3b0932640480497b78536e3db79fc
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spelling	oai:doaj.org-article:45a3b0932640480497b78536e3db79fc2021-12-02T18:23:16ZCoupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles10.1128/mSystems.00298-202379-5077https://doaj.org/article/45a3b0932640480497b78536e3db79fc2020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00298-20https://doaj.org/toc/2379-5077ABSTRACT Soil microbial community assembly is crucial for understanding the mechanisms of microbial communities that regulate ecosystem-level functioning. The relative contributions of stochastic and deterministic processes to microbial community assembly remain poorly defined, and major questions exist concerning the soil organic carbon (SOC) dynamics of microbial community assembly in deep soil. Here, the bacterial community assembly processes were explored across five soil profile depths (up to 80 cm) during a 15-year field experiment involving four fertilization regimes. We found that the bacterial community assembly was initially governed by deterministic selection in topsoil but was progressively structured by increasing stochastic dispersal with depth. The migration rate (m) and β-null deviation pattern supported the hypothesis of a relatively greater influence of dispersal in deep soil, which was correlated with bacterial community assembly by stochastic processes. These changes in the entire community assembly reflected consistent assembly processes of the two most dominant phyla, Acidobacteria and Chloroflexi. Structural equation modeling showed that soil features (pH and total phosphorus) and bacterial interactions (competition and network complexity) were significantly related to bacterial community assembly in the 0-to-10-cm and 10-to-20-cm layers. Partial Mantel tests, structural equation modeling, and random forest modeling consistently indicated a strong and significant correlation between bacterial community assemblages and SOC dynamics, implying that bacterial assembly processes would potentially suppress SOC metabolism and mineralization when the contributions of stochastic dispersal to communities increased in deeper layers. Our results have important implications for integrating bacterial community assembly processes into the predictions of SOC dynamics. IMPORTANCE We have provided a framework to better understand the mechanisms governing the balance between stochastic and deterministic processes and to integrate the shifts in community assembly processes with microbial carbon metabolism. Our study reinforced that environmental filtering and bacterial cooccurrence patterns influence the stochastic/deterministic continuum of soil bacterial community assembly and that stochasticity may act through deeper soil layers to influence carbon metabolism. Delineating theoretically the potential linkages between community assembly and SOC dynamics across a broad range of microbial systems represents an interesting topic for future research.Lu LuanChao LiangLijun ChenHaotian WangQinsong XuYuji JiangBo SunAmerican Society for Microbiologyarticlebacterial community assemblymicrobial metabolismbacterial interactionsmigration rateenvironmental filteringsoil profileMicrobiologyQR1-502ENmSystems, Vol 5, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial community assembly microbial metabolism bacterial interactions migration rate environmental filtering soil profile Microbiology QR1-502
spellingShingle	bacterial community assembly microbial metabolism bacterial interactions migration rate environmental filtering soil profile Microbiology QR1-502 Lu Luan Chao Liang Lijun Chen Haotian Wang Qinsong Xu Yuji Jiang Bo Sun Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles
description	ABSTRACT Soil microbial community assembly is crucial for understanding the mechanisms of microbial communities that regulate ecosystem-level functioning. The relative contributions of stochastic and deterministic processes to microbial community assembly remain poorly defined, and major questions exist concerning the soil organic carbon (SOC) dynamics of microbial community assembly in deep soil. Here, the bacterial community assembly processes were explored across five soil profile depths (up to 80 cm) during a 15-year field experiment involving four fertilization regimes. We found that the bacterial community assembly was initially governed by deterministic selection in topsoil but was progressively structured by increasing stochastic dispersal with depth. The migration rate (m) and β-null deviation pattern supported the hypothesis of a relatively greater influence of dispersal in deep soil, which was correlated with bacterial community assembly by stochastic processes. These changes in the entire community assembly reflected consistent assembly processes of the two most dominant phyla, Acidobacteria and Chloroflexi. Structural equation modeling showed that soil features (pH and total phosphorus) and bacterial interactions (competition and network complexity) were significantly related to bacterial community assembly in the 0-to-10-cm and 10-to-20-cm layers. Partial Mantel tests, structural equation modeling, and random forest modeling consistently indicated a strong and significant correlation between bacterial community assemblages and SOC dynamics, implying that bacterial assembly processes would potentially suppress SOC metabolism and mineralization when the contributions of stochastic dispersal to communities increased in deeper layers. Our results have important implications for integrating bacterial community assembly processes into the predictions of SOC dynamics. IMPORTANCE We have provided a framework to better understand the mechanisms governing the balance between stochastic and deterministic processes and to integrate the shifts in community assembly processes with microbial carbon metabolism. Our study reinforced that environmental filtering and bacterial cooccurrence patterns influence the stochastic/deterministic continuum of soil bacterial community assembly and that stochasticity may act through deeper soil layers to influence carbon metabolism. Delineating theoretically the potential linkages between community assembly and SOC dynamics across a broad range of microbial systems represents an interesting topic for future research.
format	article
author	Lu Luan Chao Liang Lijun Chen Haotian Wang Qinsong Xu Yuji Jiang Bo Sun
author_facet	Lu Luan Chao Liang Lijun Chen Haotian Wang Qinsong Xu Yuji Jiang Bo Sun
author_sort	Lu Luan
title	Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles
title_short	Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles
title_full	Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles
title_fullStr	Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles
title_full_unstemmed	Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles
title_sort	coupling bacterial community assembly to microbial metabolism across soil profiles
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/45a3b0932640480497b78536e3db79fc
work_keys_str_mv	AT luluan couplingbacterialcommunityassemblytomicrobialmetabolismacrosssoilprofiles AT chaoliang couplingbacterialcommunityassemblytomicrobialmetabolismacrosssoilprofiles AT lijunchen couplingbacterialcommunityassemblytomicrobialmetabolismacrosssoilprofiles AT haotianwang couplingbacterialcommunityassemblytomicrobialmetabolismacrosssoilprofiles AT qinsongxu couplingbacterialcommunityassemblytomicrobialmetabolismacrosssoilprofiles AT yujijiang couplingbacterialcommunityassemblytomicrobialmetabolismacrosssoilprofiles AT bosun couplingbacterialcommunityassemblytomicrobialmetabolismacrosssoilprofiles
_version_	1718378091756126208

Coupling Bacterial Community Assembly to Microbial Metabolism across Soil Profiles

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