Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway

Abstract Many pheromone sensing bacteria produce and detect more than one chemically distinct signal, or autoinducer. The pathways that detect these signals are typically noisy and interlocked through crosstalk and feedback. As a result, the sensing response of individual cells is described by stati...

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Autores principales:	Taylor Miller, Keval Patel, Coralis Rodriguez, Eric V. Stabb, Stephen J. Hagen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45acd0db540f47b7a78c2ba8f298ec5b
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spelling	oai:doaj.org-article:45acd0db540f47b7a78c2ba8f298ec5b2021-12-02T19:16:11ZDimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway10.1038/s41598-021-99169-02045-2322https://doaj.org/article/45acd0db540f47b7a78c2ba8f298ec5b2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99169-0https://doaj.org/toc/2045-2322Abstract Many pheromone sensing bacteria produce and detect more than one chemically distinct signal, or autoinducer. The pathways that detect these signals are typically noisy and interlocked through crosstalk and feedback. As a result, the sensing response of individual cells is described by statistical distributions that change under different combinations of signal inputs. Here we examine how signal crosstalk reshapes this response. We measure how combinations of two homoserine lactone (HSL) input signals alter the statistical distributions of individual cell responses in the AinS/R- and LuxI/R-controlled branches of the Vibrio fischeri bioluminescence pathway. We find that, while the distributions of pathway activation in individual cells vary in complex fashion with environmental conditions, these changes have a low-dimensional representation. For both the AinS/R and LuxI/R branches, the distribution of individual cell responses to mixtures of the two HSLs is effectively one-dimensional, so that a single tuning parameter can capture the full range of variability in the distributions. Combinations of crosstalking HSL signals extend the range of responses for each branch of the circuit, so that signals in combination allow population-wide distributions that are not available under a single HSL input. Dimension reduction also simplifies the problem of identifying the HSL conditions to which the pathways and their outputs are most sensitive. A comparison of the maximum sensitivity HSL conditions to actual HSL levels measured during culture growth indicates that the AinS/R and LuxI/R branches lack sensitivity to population density except during the very earliest and latest stages of growth respectively.Taylor MillerKeval PatelCoralis RodriguezEric V. StabbStephen J. HagenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taylor Miller Keval Patel Coralis Rodriguez Eric V. Stabb Stephen J. Hagen Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway
description	Abstract Many pheromone sensing bacteria produce and detect more than one chemically distinct signal, or autoinducer. The pathways that detect these signals are typically noisy and interlocked through crosstalk and feedback. As a result, the sensing response of individual cells is described by statistical distributions that change under different combinations of signal inputs. Here we examine how signal crosstalk reshapes this response. We measure how combinations of two homoserine lactone (HSL) input signals alter the statistical distributions of individual cell responses in the AinS/R- and LuxI/R-controlled branches of the Vibrio fischeri bioluminescence pathway. We find that, while the distributions of pathway activation in individual cells vary in complex fashion with environmental conditions, these changes have a low-dimensional representation. For both the AinS/R and LuxI/R branches, the distribution of individual cell responses to mixtures of the two HSLs is effectively one-dimensional, so that a single tuning parameter can capture the full range of variability in the distributions. Combinations of crosstalking HSL signals extend the range of responses for each branch of the circuit, so that signals in combination allow population-wide distributions that are not available under a single HSL input. Dimension reduction also simplifies the problem of identifying the HSL conditions to which the pathways and their outputs are most sensitive. A comparison of the maximum sensitivity HSL conditions to actual HSL levels measured during culture growth indicates that the AinS/R and LuxI/R branches lack sensitivity to population density except during the very earliest and latest stages of growth respectively.
format	article
author	Taylor Miller Keval Patel Coralis Rodriguez Eric V. Stabb Stephen J. Hagen
author_facet	Taylor Miller Keval Patel Coralis Rodriguez Eric V. Stabb Stephen J. Hagen
author_sort	Taylor Miller
title	Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway
title_short	Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway
title_full	Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway
title_fullStr	Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway
title_full_unstemmed	Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway
title_sort	dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/45acd0db540f47b7a78c2ba8f298ec5b
work_keys_str_mv	AT taylormiller dimensionreductionsimplifiestheanalysisofsignalcrosstalkinabacterialquorumsensingpathway AT kevalpatel dimensionreductionsimplifiestheanalysisofsignalcrosstalkinabacterialquorumsensingpathway AT coralisrodriguez dimensionreductionsimplifiestheanalysisofsignalcrosstalkinabacterialquorumsensingpathway AT ericvstabb dimensionreductionsimplifiestheanalysisofsignalcrosstalkinabacterialquorumsensingpathway AT stephenjhagen dimensionreductionsimplifiestheanalysisofsignalcrosstalkinabacterialquorumsensingpathway
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Dimension-reduction simplifies the analysis of signal crosstalk in a bacterial quorum sensing pathway

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