Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival
Abstract Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known...
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Nature Portfolio
2018
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oai:doaj.org-article:45af015b41e7425cbe979cb802a415552021-12-02T15:08:52ZIdentification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival10.1038/s41598-018-20929-62045-2322https://doaj.org/article/45af015b41e7425cbe979cb802a415552018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20929-6https://doaj.org/toc/2045-2322Abstract Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known to be important regulators of the stem-like features. Moreover, miRNAs have been previously proved to be promising diagnostic biomarkers in several cancers including GBM. Using global expression analysis of miRNAs in 10 paired in-vitro as well as in-vivo characterized primary GSC and non-stem glioblastoma cultures, we identified a miRNA signature associated with the stem-like phenotype in GBM. 51 most deregulated miRNAs classified the cell cultures into GSC and non-stem cell clusters and identified a subgroup of GSC cultures with more pronounced stem-cell characteristics. The importance of the identified miRNA signature was further supported by demonstrating that a Risk Score based on the expression of seven miRNAs overexpressed in GSC predicted overall survival in GBM patients in the TCGA dataset independently of the IDH1 status. In summary, we identified miRNAs differentially expressed in GSCs and described their association with GBM patient survival. We propose that these miRNAs participate on GSC features and could represent helpful prognostic markers and potential therapeutic targets in GBM.Jiri SanaPetr BusekPavel FadrusAndrej BesseLenka RadovaMarek VeceraStefan ReguliLucie Stollinova SromovaMarek HilserRadim LipinaRadek LakomyLeos KrenMartin SmrckaAleksi SedoOndrej SlabyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Jiri Sana Petr Busek Pavel Fadrus Andrej Besse Lenka Radova Marek Vecera Stefan Reguli Lucie Stollinova Sromova Marek Hilser Radim Lipina Radek Lakomy Leos Kren Martin Smrcka Aleksi Sedo Ondrej Slaby Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival |
| description |
Abstract Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known to be important regulators of the stem-like features. Moreover, miRNAs have been previously proved to be promising diagnostic biomarkers in several cancers including GBM. Using global expression analysis of miRNAs in 10 paired in-vitro as well as in-vivo characterized primary GSC and non-stem glioblastoma cultures, we identified a miRNA signature associated with the stem-like phenotype in GBM. 51 most deregulated miRNAs classified the cell cultures into GSC and non-stem cell clusters and identified a subgroup of GSC cultures with more pronounced stem-cell characteristics. The importance of the identified miRNA signature was further supported by demonstrating that a Risk Score based on the expression of seven miRNAs overexpressed in GSC predicted overall survival in GBM patients in the TCGA dataset independently of the IDH1 status. In summary, we identified miRNAs differentially expressed in GSCs and described their association with GBM patient survival. We propose that these miRNAs participate on GSC features and could represent helpful prognostic markers and potential therapeutic targets in GBM. |
| format |
article |
| author |
Jiri Sana Petr Busek Pavel Fadrus Andrej Besse Lenka Radova Marek Vecera Stefan Reguli Lucie Stollinova Sromova Marek Hilser Radim Lipina Radek Lakomy Leos Kren Martin Smrcka Aleksi Sedo Ondrej Slaby |
| author_facet |
Jiri Sana Petr Busek Pavel Fadrus Andrej Besse Lenka Radova Marek Vecera Stefan Reguli Lucie Stollinova Sromova Marek Hilser Radim Lipina Radek Lakomy Leos Kren Martin Smrcka Aleksi Sedo Ondrej Slaby |
| author_sort |
Jiri Sana |
| title |
Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival |
| title_short |
Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival |
| title_full |
Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival |
| title_fullStr |
Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival |
| title_full_unstemmed |
Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival |
| title_sort |
identification of micrornas differentially expressed in glioblastoma stem-like cells and their association with patient survival |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/45af015b41e7425cbe979cb802a41555 |
| work_keys_str_mv |
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