Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae

Abstract Evolutionary arms races are broadly prevalent among organisms including bacteria, which have evolved defensive strategies against various attackers. A common microbial aggression mechanism is the type VI secretion system (T6SS), a contact-dependent bacterial weapon used to deliver toxic eff...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cristian V. Crisan, Holly L. Nichols, Sophia Wiesenfeld, Gabi Steinbach, Peter J. Yunker, Brian K. Hammer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45b2fb3fe38648819f5faeb56a31fd42
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:45b2fb3fe38648819f5faeb56a31fd42
record_format	dspace
spelling	oai:doaj.org-article:45b2fb3fe38648819f5faeb56a31fd422021-12-02T14:06:19ZGlucose confers protection to Escherichia coli against contact killing by Vibrio cholerae10.1038/s41598-021-81813-42045-2322https://doaj.org/article/45b2fb3fe38648819f5faeb56a31fd422021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81813-4https://doaj.org/toc/2045-2322Abstract Evolutionary arms races are broadly prevalent among organisms including bacteria, which have evolved defensive strategies against various attackers. A common microbial aggression mechanism is the type VI secretion system (T6SS), a contact-dependent bacterial weapon used to deliver toxic effector proteins into adjacent target cells. Sibling cells constitutively express immunity proteins that neutralize effectors. However, less is known about factors that protect non-sibling bacteria from T6SS attacks independently of cognate immunity proteins. In this study, we observe that human Escherichia coli commensal strains sensitive to T6SS attacks from Vibrio cholerae are protected when co-cultured with glucose. We confirm that glucose does not impair V. cholerae T6SS activity. Instead, we find that cells lacking the cAMP receptor protein (CRP), which regulates expression of hundreds of genes in response to glucose, survive significantly better against V. cholerae T6SS attacks even in the absence of glucose. Finally, we show that the glucose-mediated T6SS protection varies with different targets and killers. Our findings highlight the first example of an extracellular small molecule modulating a genetically controlled response for protection against T6SS attacks. This discovery may have major implications for microbial interactions during pathogen-host colonization and survival of bacteria in environmental communities.Cristian V. CrisanHolly L. NicholsSophia WiesenfeldGabi SteinbachPeter J. YunkerBrian K. HammerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristian V. Crisan Holly L. Nichols Sophia Wiesenfeld Gabi Steinbach Peter J. Yunker Brian K. Hammer Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae
description	Abstract Evolutionary arms races are broadly prevalent among organisms including bacteria, which have evolved defensive strategies against various attackers. A common microbial aggression mechanism is the type VI secretion system (T6SS), a contact-dependent bacterial weapon used to deliver toxic effector proteins into adjacent target cells. Sibling cells constitutively express immunity proteins that neutralize effectors. However, less is known about factors that protect non-sibling bacteria from T6SS attacks independently of cognate immunity proteins. In this study, we observe that human Escherichia coli commensal strains sensitive to T6SS attacks from Vibrio cholerae are protected when co-cultured with glucose. We confirm that glucose does not impair V. cholerae T6SS activity. Instead, we find that cells lacking the cAMP receptor protein (CRP), which regulates expression of hundreds of genes in response to glucose, survive significantly better against V. cholerae T6SS attacks even in the absence of glucose. Finally, we show that the glucose-mediated T6SS protection varies with different targets and killers. Our findings highlight the first example of an extracellular small molecule modulating a genetically controlled response for protection against T6SS attacks. This discovery may have major implications for microbial interactions during pathogen-host colonization and survival of bacteria in environmental communities.
format	article
author	Cristian V. Crisan Holly L. Nichols Sophia Wiesenfeld Gabi Steinbach Peter J. Yunker Brian K. Hammer
author_facet	Cristian V. Crisan Holly L. Nichols Sophia Wiesenfeld Gabi Steinbach Peter J. Yunker Brian K. Hammer
author_sort	Cristian V. Crisan
title	Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae
title_short	Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae
title_full	Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae
title_fullStr	Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae
title_full_unstemmed	Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae
title_sort	glucose confers protection to escherichia coli against contact killing by vibrio cholerae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/45b2fb3fe38648819f5faeb56a31fd42
work_keys_str_mv	AT cristianvcrisan glucoseconfersprotectiontoescherichiacoliagainstcontactkillingbyvibriocholerae AT hollylnichols glucoseconfersprotectiontoescherichiacoliagainstcontactkillingbyvibriocholerae AT sophiawiesenfeld glucoseconfersprotectiontoescherichiacoliagainstcontactkillingbyvibriocholerae AT gabisteinbach glucoseconfersprotectiontoescherichiacoliagainstcontactkillingbyvibriocholerae AT peterjyunker glucoseconfersprotectiontoescherichiacoliagainstcontactkillingbyvibriocholerae AT briankhammer glucoseconfersprotectiontoescherichiacoliagainstcontactkillingbyvibriocholerae
_version_	1718392015965650944

Glucose confers protection to Escherichia coli against contact killing by Vibrio cholerae

Ejemplares similares