MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols

Oral uptake is the primary route of human bisphenol exposure, resulting in an exposure of the intestinal microbiota and intestine-associated immune cells. Therefore, we compared the impact of bisphenol A (BPA), bisphenol F (BPF) and bisphenol S (BPS) on (i) intestinal microbiota, (ii) microbiota-med...

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Autores principales:	J.L. Krause, B. Engelmann, U. Nunes da Rocha, A. Pierzchalski, H.D. Chang, A.C. Zenclussen, M. von Bergen, U. Rolle-Kampczyk, G. Herberth
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Bisphenols Human intestinal microbiota In vitro model Batch culture MAIT cells Immunomodulation Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/45b67609f69147f98fd5165def431f5e
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spelling	oai:doaj.org-article:45b67609f69147f98fd5165def431f5e2021-11-22T04:16:55ZMAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols0160-412010.1016/j.envint.2021.106985https://doaj.org/article/45b67609f69147f98fd5165def431f5e2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0160412021006103https://doaj.org/toc/0160-4120Oral uptake is the primary route of human bisphenol exposure, resulting in an exposure of the intestinal microbiota and intestine-associated immune cells. Therefore, we compared the impact of bisphenol A (BPA), bisphenol F (BPF) and bisphenol S (BPS) on (i) intestinal microbiota, (ii) microbiota-mediated immunomodulatory effects and (iii) direct effects on mucosal-associated invariant T (MAIT) cells in vitro. We acutely exposed human fecal microbiota, Bacteroides thetaiotaomicron and Escherichia coli to BPA and its analogues BPF and BPS referring to the European tolerable daily intake (TDI), i.e. 2.3 µg/mL, 28.3 µg/mL and 354.0 µg/mL. Growth and viability of E. coli was most susceptible to BPF, whereas B. thetaiotaomicron and fecal microbiota were affected by BPA > BPF > BPS. At 354.0 µg/mL bisphenols altered microbial diversity in compound-specific manner and modulated microbial metabolism, with BPA already acting on metabolism at 28.3 µg/mL. Microbiota-mediated effects on MAIT cells were observed for the individual bacteria at 354.0 µg/mL only. However, BPA and BPF directly modulated MAIT cell responses at low concentrations, whereby bisphenols at concentrations equivalent for the current TDI had no modulatory effects for microbiota or for MAIT cells. Our findings indicate that acute bisphenol exposure may alter microbial metabolism and impact directly on immune cells.J.L. KrauseB. EngelmannU. Nunes da RochaA. PierzchalskiH.D. ChangA.C. ZenclussenM. von BergenU. Rolle-KampczykG. HerberthElsevierarticleBisphenolsHuman intestinal microbiotaIn vitro modelBatch cultureMAIT cellsImmunomodulationEnvironmental sciencesGE1-350ENEnvironment International, Vol 158, Iss , Pp 106985- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bisphenols Human intestinal microbiota In vitro model Batch culture MAIT cells Immunomodulation Environmental sciences GE1-350
spellingShingle	Bisphenols Human intestinal microbiota In vitro model Batch culture MAIT cells Immunomodulation Environmental sciences GE1-350 J.L. Krause B. Engelmann U. Nunes da Rocha A. Pierzchalski H.D. Chang A.C. Zenclussen M. von Bergen U. Rolle-Kampczyk G. Herberth MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
description	Oral uptake is the primary route of human bisphenol exposure, resulting in an exposure of the intestinal microbiota and intestine-associated immune cells. Therefore, we compared the impact of bisphenol A (BPA), bisphenol F (BPF) and bisphenol S (BPS) on (i) intestinal microbiota, (ii) microbiota-mediated immunomodulatory effects and (iii) direct effects on mucosal-associated invariant T (MAIT) cells in vitro. We acutely exposed human fecal microbiota, Bacteroides thetaiotaomicron and Escherichia coli to BPA and its analogues BPF and BPS referring to the European tolerable daily intake (TDI), i.e. 2.3 µg/mL, 28.3 µg/mL and 354.0 µg/mL. Growth and viability of E. coli was most susceptible to BPF, whereas B. thetaiotaomicron and fecal microbiota were affected by BPA > BPF > BPS. At 354.0 µg/mL bisphenols altered microbial diversity in compound-specific manner and modulated microbial metabolism, with BPA already acting on metabolism at 28.3 µg/mL. Microbiota-mediated effects on MAIT cells were observed for the individual bacteria at 354.0 µg/mL only. However, BPA and BPF directly modulated MAIT cell responses at low concentrations, whereby bisphenols at concentrations equivalent for the current TDI had no modulatory effects for microbiota or for MAIT cells. Our findings indicate that acute bisphenol exposure may alter microbial metabolism and impact directly on immune cells.
format	article
author	J.L. Krause B. Engelmann U. Nunes da Rocha A. Pierzchalski H.D. Chang A.C. Zenclussen M. von Bergen U. Rolle-Kampczyk G. Herberth
author_facet	J.L. Krause B. Engelmann U. Nunes da Rocha A. Pierzchalski H.D. Chang A.C. Zenclussen M. von Bergen U. Rolle-Kampczyk G. Herberth
author_sort	J.L. Krause
title	MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
title_short	MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
title_full	MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
title_fullStr	MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
title_full_unstemmed	MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
title_sort	mait cell activation is reduced by direct and microbiota-mediated exposure to bisphenols
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/45b67609f69147f98fd5165def431f5e
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_version_	1718418218763157504

MAIT cell activation is reduced by direct and microbiota-mediated exposure to bisphenols

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