Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue

Abstract Thoroughbreds have high maximal oxygen consumption and show hypoxemia and hypercapnia during intense exercise, suggesting that the peripheral environment in skeletal muscle may be severe. Changes in metabolites following extreme alterations in the muscle environment in horses after exercise...

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Autores principales:	Hajime Ohmura, Kazutaka Mukai, Yuji Takahashi, Toshiyuki Takahashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45b77d4faf5a4a6b9094903dbed1ed00
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spelling	oai:doaj.org-article:45b77d4faf5a4a6b9094903dbed1ed002021-12-02T16:53:00ZMetabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue10.1038/s41598-021-90834-y2045-2322https://doaj.org/article/45b77d4faf5a4a6b9094903dbed1ed002021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90834-yhttps://doaj.org/toc/2045-2322Abstract Thoroughbreds have high maximal oxygen consumption and show hypoxemia and hypercapnia during intense exercise, suggesting that the peripheral environment in skeletal muscle may be severe. Changes in metabolites following extreme alterations in the muscle environment in horses after exercise may provide useful evidence. We compared the muscle metabolites before and after supramaximal exercise to fatigue in horses. Six well-trained horses ran until exhaustion in incremental exercise tests. Biopsy samples were obtained from the gluteus medius muscle before and immediately after exercise for capillary electrophoresis–mass spectrometry analysis. In the incremental exercise test, the total running time and speed of the last step were 10.4 ± 1.3 (mean ± standard deviation) min and 12.7 ± 0.5 m/s, respectively. Of 73 metabolites, 18 and 11 were significantly increased and decreased after exercise, respectively. The heat map of the hierarchical cluster analysis of muscle metabolites showed that changes in metabolites were clearly distinguishable before and after exercise. Strenuous exercise increased many metabolites in the glycolytic pathway and the tricarboxylic acid cycle in skeletal muscle. Targeted metabolomic analysis of skeletal muscle may clarify the intramuscular environment caused by exercise and explain the response of working muscles to strenuous exercise that induces hypoxemia and hypercapnia in Thoroughbred horses.Hajime OhmuraKazutaka MukaiYuji TakahashiToshiyuki TakahashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hajime Ohmura Kazutaka Mukai Yuji Takahashi Toshiyuki Takahashi Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue
description	Abstract Thoroughbreds have high maximal oxygen consumption and show hypoxemia and hypercapnia during intense exercise, suggesting that the peripheral environment in skeletal muscle may be severe. Changes in metabolites following extreme alterations in the muscle environment in horses after exercise may provide useful evidence. We compared the muscle metabolites before and after supramaximal exercise to fatigue in horses. Six well-trained horses ran until exhaustion in incremental exercise tests. Biopsy samples were obtained from the gluteus medius muscle before and immediately after exercise for capillary electrophoresis–mass spectrometry analysis. In the incremental exercise test, the total running time and speed of the last step were 10.4 ± 1.3 (mean ± standard deviation) min and 12.7 ± 0.5 m/s, respectively. Of 73 metabolites, 18 and 11 were significantly increased and decreased after exercise, respectively. The heat map of the hierarchical cluster analysis of muscle metabolites showed that changes in metabolites were clearly distinguishable before and after exercise. Strenuous exercise increased many metabolites in the glycolytic pathway and the tricarboxylic acid cycle in skeletal muscle. Targeted metabolomic analysis of skeletal muscle may clarify the intramuscular environment caused by exercise and explain the response of working muscles to strenuous exercise that induces hypoxemia and hypercapnia in Thoroughbred horses.
format	article
author	Hajime Ohmura Kazutaka Mukai Yuji Takahashi Toshiyuki Takahashi
author_facet	Hajime Ohmura Kazutaka Mukai Yuji Takahashi Toshiyuki Takahashi
author_sort	Hajime Ohmura
title	Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue
title_short	Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue
title_full	Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue
title_fullStr	Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue
title_full_unstemmed	Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue
title_sort	metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/45b77d4faf5a4a6b9094903dbed1ed00
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_version_	1718382853026217984

Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue

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