Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves

Abstract Force-displacement (F-Z) curves are the most commonly used Atomic Force Microscopy (AFM) mode to measure the local, nanoscale elastic properties of soft materials like living cells. Yet a theoretical framework has been lacking that allows the post-processing of F-Z data to extract their vis...

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Autores principales:	Yuri M. Efremov, Wen-Horng Wang, Shana D. Hardy, Robert L. Geahlen, Arvind Raman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45b8218a6d8d411c94cc58c9b938bda2
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spelling	oai:doaj.org-article:45b8218a6d8d411c94cc58c9b938bda22021-12-02T11:40:23ZMeasuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves10.1038/s41598-017-01784-32045-2322https://doaj.org/article/45b8218a6d8d411c94cc58c9b938bda22017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01784-3https://doaj.org/toc/2045-2322Abstract Force-displacement (F-Z) curves are the most commonly used Atomic Force Microscopy (AFM) mode to measure the local, nanoscale elastic properties of soft materials like living cells. Yet a theoretical framework has been lacking that allows the post-processing of F-Z data to extract their viscoelastic constitutive parameters. Here, we propose a new method to extract nanoscale viscoelastic properties of soft samples like living cells and hydrogels directly from conventional AFM F-Z experiments, thereby creating a common platform for the analysis of cell elastic and viscoelastic properties with arbitrary linear constitutive relations. The method based on the elastic-viscoelastic correspondence principle was validated using finite element (FE) simulations and by comparison with the existed AFM techniques on living cells and hydrogels. The method also allows a discrimination of which viscoelastic relaxation model, for example, standard linear solid (SLS) or power-law rheology (PLR), best suits the experimental data. The method was used to extract the viscoelastic properties of benign and cancerous cell lines (NIH 3T3 fibroblasts, NMuMG epithelial, MDA-MB-231 and MCF-7 breast cancer cells). Finally, we studied the changes in viscoelastic properties related to tumorigenesis including TGF-β induced epithelial-to-mesenchymal transition on NMuMG cells and Syk expression induced phenotype changes in MDA-MB-231 cells.Yuri M. EfremovWen-Horng WangShana D. HardyRobert L. GeahlenArvind RamanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuri M. Efremov Wen-Horng Wang Shana D. Hardy Robert L. Geahlen Arvind Raman Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves
description	Abstract Force-displacement (F-Z) curves are the most commonly used Atomic Force Microscopy (AFM) mode to measure the local, nanoscale elastic properties of soft materials like living cells. Yet a theoretical framework has been lacking that allows the post-processing of F-Z data to extract their viscoelastic constitutive parameters. Here, we propose a new method to extract nanoscale viscoelastic properties of soft samples like living cells and hydrogels directly from conventional AFM F-Z experiments, thereby creating a common platform for the analysis of cell elastic and viscoelastic properties with arbitrary linear constitutive relations. The method based on the elastic-viscoelastic correspondence principle was validated using finite element (FE) simulations and by comparison with the existed AFM techniques on living cells and hydrogels. The method also allows a discrimination of which viscoelastic relaxation model, for example, standard linear solid (SLS) or power-law rheology (PLR), best suits the experimental data. The method was used to extract the viscoelastic properties of benign and cancerous cell lines (NIH 3T3 fibroblasts, NMuMG epithelial, MDA-MB-231 and MCF-7 breast cancer cells). Finally, we studied the changes in viscoelastic properties related to tumorigenesis including TGF-β induced epithelial-to-mesenchymal transition on NMuMG cells and Syk expression induced phenotype changes in MDA-MB-231 cells.
format	article
author	Yuri M. Efremov Wen-Horng Wang Shana D. Hardy Robert L. Geahlen Arvind Raman
author_facet	Yuri M. Efremov Wen-Horng Wang Shana D. Hardy Robert L. Geahlen Arvind Raman
author_sort	Yuri M. Efremov
title	Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves
title_short	Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves
title_full	Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves
title_fullStr	Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves
title_full_unstemmed	Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves
title_sort	measuring nanoscale viscoelastic parameters of cells directly from afm force-displacement curves
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/45b8218a6d8d411c94cc58c9b938bda2
work_keys_str_mv	AT yurimefremov measuringnanoscaleviscoelasticparametersofcellsdirectlyfromafmforcedisplacementcurves AT wenhorngwang measuringnanoscaleviscoelasticparametersofcellsdirectlyfromafmforcedisplacementcurves AT shanadhardy measuringnanoscaleviscoelasticparametersofcellsdirectlyfromafmforcedisplacementcurves AT robertlgeahlen measuringnanoscaleviscoelasticparametersofcellsdirectlyfromafmforcedisplacementcurves AT arvindraman measuringnanoscaleviscoelasticparametersofcellsdirectlyfromafmforcedisplacementcurves
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Measuring nanoscale viscoelastic parameters of cells directly from AFM force-displacement curves

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