Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding

Abstract Australia has over 30 Panicum spp. (panic grass) including several non-native species that cause crop and pasture loss and hepatogenous photosensitisation in livestock. It is critical to correctly identify them at the species level to facilitate the development of appropriate management str...

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Autores principales:	Yuchi Chen, Xiaocheng Zhu, Panayiotis Loukopoulos, Leslie A. Weston, David E. Albrecht, Jane C. Quinn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45be2f043d8d44759eb5edf9c3dcfc40
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spelling	oai:doaj.org-article:45be2f043d8d44759eb5edf9c3dcfc402021-12-02T18:49:16ZGenotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding10.1038/s41598-021-95610-62045-2322https://doaj.org/article/45be2f043d8d44759eb5edf9c3dcfc402021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95610-6https://doaj.org/toc/2045-2322Abstract Australia has over 30 Panicum spp. (panic grass) including several non-native species that cause crop and pasture loss and hepatogenous photosensitisation in livestock. It is critical to correctly identify them at the species level to facilitate the development of appropriate management strategies for efficacious control of Panicum grasses in crops, fallows and pastures. Currently, identification of Panicum spp. relies on morphological examination of the reproductive structures, but this approach is only useful for flowering specimens and requires significant taxonomic expertise. To overcome this limitation, we used multi-locus DNA barcoding for the identification of ten selected Panicum spp. found in Australia. With the exception of P. buncei, other native Australian Panicum were genetically separated at the species level and distinguished from non-native species. One nuclear (ITS) and two chloroplast regions (matK and trnL intron-trnF) were identified with varying facility for DNA barcode separation of the Panicum species. Concatenation of sequences from ITS, matK and trnL intron-trnF regions provided clear separation of eight regionally collected species, with a maximum intraspecific distance of 0.22% and minimum interspecific distance of 0.33%. Two of three non-native Panicum species exhibited a smaller genome size compared to native species evaluated, and we speculate that this may be associated with biological advantages impacting invasion of non-native Panicum species in novel locations. We conclude that multi-locus DNA barcoding, in combination with traditional taxonomic identification, provides an accurate and cost-effective adjunctive tool for further distinguishing Panicum spp. at the species level.Yuchi ChenXiaocheng ZhuPanayiotis LoukopoulosLeslie A. WestonDavid E. AlbrechtJane C. QuinnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuchi Chen Xiaocheng Zhu Panayiotis Loukopoulos Leslie A. Weston David E. Albrecht Jane C. Quinn Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding
description	Abstract Australia has over 30 Panicum spp. (panic grass) including several non-native species that cause crop and pasture loss and hepatogenous photosensitisation in livestock. It is critical to correctly identify them at the species level to facilitate the development of appropriate management strategies for efficacious control of Panicum grasses in crops, fallows and pastures. Currently, identification of Panicum spp. relies on morphological examination of the reproductive structures, but this approach is only useful for flowering specimens and requires significant taxonomic expertise. To overcome this limitation, we used multi-locus DNA barcoding for the identification of ten selected Panicum spp. found in Australia. With the exception of P. buncei, other native Australian Panicum were genetically separated at the species level and distinguished from non-native species. One nuclear (ITS) and two chloroplast regions (matK and trnL intron-trnF) were identified with varying facility for DNA barcode separation of the Panicum species. Concatenation of sequences from ITS, matK and trnL intron-trnF regions provided clear separation of eight regionally collected species, with a maximum intraspecific distance of 0.22% and minimum interspecific distance of 0.33%. Two of three non-native Panicum species exhibited a smaller genome size compared to native species evaluated, and we speculate that this may be associated with biological advantages impacting invasion of non-native Panicum species in novel locations. We conclude that multi-locus DNA barcoding, in combination with traditional taxonomic identification, provides an accurate and cost-effective adjunctive tool for further distinguishing Panicum spp. at the species level.
format	article
author	Yuchi Chen Xiaocheng Zhu Panayiotis Loukopoulos Leslie A. Weston David E. Albrecht Jane C. Quinn
author_facet	Yuchi Chen Xiaocheng Zhu Panayiotis Loukopoulos Leslie A. Weston David E. Albrecht Jane C. Quinn
author_sort	Yuchi Chen
title	Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding
title_short	Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding
title_full	Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding
title_fullStr	Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding
title_full_unstemmed	Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding
title_sort	genotypic identification of panicum spp. in new south wales, australia using dna barcoding
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/45be2f043d8d44759eb5edf9c3dcfc40
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Genotypic identification of Panicum spp. in New South Wales, Australia using DNA barcoding

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