Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells

Abstract Single-cell pH-sensing and accurate detection and label-free fast identification of cancer-cells are two long-standing pursuits in cell and life science, as intracellular pH plays a crucial role in many cellular events and fates, while the latter is vital for early cancer theranostics. Nume...

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Autores principales:	Hui Hou, Yangyang Zhao, Chuanping Li, Minmin Wang, Xiaolong Xu, Yongdong Jin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45c2719c883240cea6e886d7db64d92e
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spelling	oai:doaj.org-article:45c2719c883240cea6e886d7db64d92e2021-12-02T16:08:20ZSingle-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells10.1038/s41598-017-01956-12045-2322https://doaj.org/article/45c2719c883240cea6e886d7db64d92e2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01956-1https://doaj.org/toc/2045-2322Abstract Single-cell pH-sensing and accurate detection and label-free fast identification of cancer-cells are two long-standing pursuits in cell and life science, as intracellular pH plays a crucial role in many cellular events and fates, while the latter is vital for early cancer theranostics. Numerous methods based on functionalized nanoparticles and fluorescence probes have been developed for cell pH-sensing, but are often hindered for single-cell studies by their main drawbacks of complicated probe preparation and labeling, low sensitivity and poor reproducibility. Here we report a simple and reliable method for single-cell pH imaging and sensing by innovative combined use of UV-Vis microspectroscopy and common pH indicators. Accurate and sensitive pH detection on single-cell or sub-cell level with good reproducibility is achieved by the method, which enables facile single-cell pH profiling and label-free rapid identification of cancer-cells (due to distinguishable intracellular pH levels) for early cancer diagnosis, and may open a new avenue for pH-related single-cell studies.Hui HouYangyang ZhaoChuanping LiMinmin WangXiaolong XuYongdong JinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui Hou Yangyang Zhao Chuanping Li Minmin Wang Xiaolong Xu Yongdong Jin Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells
description	Abstract Single-cell pH-sensing and accurate detection and label-free fast identification of cancer-cells are two long-standing pursuits in cell and life science, as intracellular pH plays a crucial role in many cellular events and fates, while the latter is vital for early cancer theranostics. Numerous methods based on functionalized nanoparticles and fluorescence probes have been developed for cell pH-sensing, but are often hindered for single-cell studies by their main drawbacks of complicated probe preparation and labeling, low sensitivity and poor reproducibility. Here we report a simple and reliable method for single-cell pH imaging and sensing by innovative combined use of UV-Vis microspectroscopy and common pH indicators. Accurate and sensitive pH detection on single-cell or sub-cell level with good reproducibility is achieved by the method, which enables facile single-cell pH profiling and label-free rapid identification of cancer-cells (due to distinguishable intracellular pH levels) for early cancer diagnosis, and may open a new avenue for pH-related single-cell studies.
format	article
author	Hui Hou Yangyang Zhao Chuanping Li Minmin Wang Xiaolong Xu Yongdong Jin
author_facet	Hui Hou Yangyang Zhao Chuanping Li Minmin Wang Xiaolong Xu Yongdong Jin
author_sort	Hui Hou
title	Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells
title_short	Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells
title_full	Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells
title_fullStr	Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells
title_full_unstemmed	Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells
title_sort	single-cell ph imaging and detection for ph profiling and label-free rapid identification of cancer-cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/45c2719c883240cea6e886d7db64d92e
work_keys_str_mv	AT huihou singlecellphimaginganddetectionforphprofilingandlabelfreerapididentificationofcancercells AT yangyangzhao singlecellphimaginganddetectionforphprofilingandlabelfreerapididentificationofcancercells AT chuanpingli singlecellphimaginganddetectionforphprofilingandlabelfreerapididentificationofcancercells AT minminwang singlecellphimaginganddetectionforphprofilingandlabelfreerapididentificationofcancercells AT xiaolongxu singlecellphimaginganddetectionforphprofilingandlabelfreerapididentificationofcancercells AT yongdongjin singlecellphimaginganddetectionforphprofilingandlabelfreerapididentificationofcancercells
_version_	1718384525437829120

Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells

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