Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains

Methicillin-resistant strains of <i>Staphylococcus aureus</i> (MRSA) have developed resistance to most β-lactam antibiotics and have become a global health issue. In this work, we analyzed the impact of a rotating magnetic field (RMF) of well-defined and strictly controlled characteristi...

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Autores principales:	Marta Woroszyło, Daria Ciecholewska-Juśko, Adam Junka, Radosław Drozd, Marcin Wardach, Paweł Migdał, Patrycja Szymczyk-Ziółkowska, Daniel Styburski, Karol Fijałkowski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiotics antibiotic resistance MRSA <i>Staphylococcus aureus</i> rotating magnetic field Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/45c4f5ac65da4d89b066c1c91ca9d467
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spelling	oai:doaj.org-article:45c4f5ac65da4d89b066c1c91ca9d4672021-11-25T17:56:16ZRotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains10.3390/ijms2222123971422-00671661-6596https://doaj.org/article/45c4f5ac65da4d89b066c1c91ca9d4672021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12397https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Methicillin-resistant strains of <i>Staphylococcus aureus</i> (MRSA) have developed resistance to most β-lactam antibiotics and have become a global health issue. In this work, we analyzed the impact of a rotating magnetic field (RMF) of well-defined and strictly controlled characteristics coupled with β-lactam antibiotics against a total of 28 methicillin-resistant and sensitive <i>S. aureus</i> strains. The results indicate that the application of RMF combined with β-lactam antibiotics correlated with favorable changes in growth inhibition zones or in minimal inhibitory concentrations of the antibiotics compared to controls unexposed to RMF. Fluorescence microscopy indicated a drop in the relative number of cells with intact cell walls after exposure to RMF. These findings were additionally supported by the use of SEM and TEM microscopy, which revealed morphological alterations of RMF-exposed cells manifested by change of shape, drop in cell wall density and cytoplasm condensation. The obtained results indicate that the originally limited impact of β-lactam antibiotics in MRSA is boosted by the disturbances caused by RMF in the bacterial cell walls. Taking into account the high clinical need for new therapeutic options, effective against MRSA, the data presented in this study have high developmental potential and could serve as a basis for new treatment options for MRSA infections.Marta WoroszyłoDaria Ciecholewska-JuśkoAdam JunkaRadosław DrozdMarcin WardachPaweł MigdałPatrycja Szymczyk-ZiółkowskaDaniel StyburskiKarol FijałkowskiMDPI AGarticleantibioticsantibiotic resistanceMRSA<i>Staphylococcus aureus</i>rotating magnetic fieldBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12397, p 12397 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotics antibiotic resistance MRSA <i>Staphylococcus aureus</i> rotating magnetic field Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	antibiotics antibiotic resistance MRSA <i>Staphylococcus aureus</i> rotating magnetic field Biology (General) QH301-705.5 Chemistry QD1-999 Marta Woroszyło Daria Ciecholewska-Juśko Adam Junka Radosław Drozd Marcin Wardach Paweł Migdał Patrycja Szymczyk-Ziółkowska Daniel Styburski Karol Fijałkowski Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains
description	Methicillin-resistant strains of <i>Staphylococcus aureus</i> (MRSA) have developed resistance to most β-lactam antibiotics and have become a global health issue. In this work, we analyzed the impact of a rotating magnetic field (RMF) of well-defined and strictly controlled characteristics coupled with β-lactam antibiotics against a total of 28 methicillin-resistant and sensitive <i>S. aureus</i> strains. The results indicate that the application of RMF combined with β-lactam antibiotics correlated with favorable changes in growth inhibition zones or in minimal inhibitory concentrations of the antibiotics compared to controls unexposed to RMF. Fluorescence microscopy indicated a drop in the relative number of cells with intact cell walls after exposure to RMF. These findings were additionally supported by the use of SEM and TEM microscopy, which revealed morphological alterations of RMF-exposed cells manifested by change of shape, drop in cell wall density and cytoplasm condensation. The obtained results indicate that the originally limited impact of β-lactam antibiotics in MRSA is boosted by the disturbances caused by RMF in the bacterial cell walls. Taking into account the high clinical need for new therapeutic options, effective against MRSA, the data presented in this study have high developmental potential and could serve as a basis for new treatment options for MRSA infections.
format	article
author	Marta Woroszyło Daria Ciecholewska-Juśko Adam Junka Radosław Drozd Marcin Wardach Paweł Migdał Patrycja Szymczyk-Ziółkowska Daniel Styburski Karol Fijałkowski
author_facet	Marta Woroszyło Daria Ciecholewska-Juśko Adam Junka Radosław Drozd Marcin Wardach Paweł Migdał Patrycja Szymczyk-Ziółkowska Daniel Styburski Karol Fijałkowski
author_sort	Marta Woroszyło
title	Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains
title_short	Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains
title_full	Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains
title_fullStr	Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains
title_full_unstemmed	Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains
title_sort	rotating magnetic field increases β-lactam antibiotic susceptibility of methicillin-resistant <i>staphylococcus aureus</i> strains
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/45c4f5ac65da4d89b066c1c91ca9d467
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Rotating Magnetic Field Increases β-Lactam Antibiotic Susceptibility of Methicillin-Resistant <i>Staphylococcus aureus</i> Strains

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