Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.

<h4>Background</h4>Herpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions o...

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Autores principales:	Sarah Glorieux, Claus Bachert, Herman W Favoreel, Annelies P Vandekerckhove, Lennert Steukers, Anamaria Rekecki, Wim Van den Broeck, Joline Goossens, Siska Croubels, Reginald F Clayton, Hans J Nauwynck
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45c9e670fca84478826f38a952d43c81
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spelling	oai:doaj.org-article:45c9e670fca84478826f38a952d43c812021-11-18T06:50:10ZHerpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.1932-620310.1371/journal.pone.0022160https://doaj.org/article/45c9e670fca84478826f38a952d43c812011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21789229/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Herpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions of herpesviruses with human mucosa, the locus of infection, remain poorly understood and the underlying mechanisms in stromal infection remain controversial.<h4>Methodology/principal findings</h4>A human model consisting of nasal respiratory mucosa explants was characterised. Viability and integrity were examined during 96 h of cultivation. HSV1-mucosa interactions were analysed. In particular, we investigated whether HSV1 is able to reach the stroma. Explant viability and integrity remained preserved. HSV1 induced rounding up and loosening of epithelial cells with very few apoptotic and necrotic cells observed. Following 16-24 h of infection, HSV1 penetrated the basement membrane and replicated in the underlying lamina propria.<h4>Conclusions/significance</h4>This human explant model can be used to study virus-mucosa interactions and viral mucosal invasion mechanisms. Using this model, our results provide a novel insight into the HSV1 stromal invasion mechanism and for the first time directly demonstrate that HSV1 can penetrate the basement membrane.Sarah GlorieuxClaus BachertHerman W FavoreelAnnelies P VandekerckhoveLennert SteukersAnamaria RekeckiWim Van den BroeckJoline GoossensSiska CroubelsReginald F ClaytonHans J NauwynckPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22160 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah Glorieux Claus Bachert Herman W Favoreel Annelies P Vandekerckhove Lennert Steukers Anamaria Rekecki Wim Van den Broeck Joline Goossens Siska Croubels Reginald F Clayton Hans J Nauwynck Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.
description	<h4>Background</h4>Herpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions of herpesviruses with human mucosa, the locus of infection, remain poorly understood and the underlying mechanisms in stromal infection remain controversial.<h4>Methodology/principal findings</h4>A human model consisting of nasal respiratory mucosa explants was characterised. Viability and integrity were examined during 96 h of cultivation. HSV1-mucosa interactions were analysed. In particular, we investigated whether HSV1 is able to reach the stroma. Explant viability and integrity remained preserved. HSV1 induced rounding up and loosening of epithelial cells with very few apoptotic and necrotic cells observed. Following 16-24 h of infection, HSV1 penetrated the basement membrane and replicated in the underlying lamina propria.<h4>Conclusions/significance</h4>This human explant model can be used to study virus-mucosa interactions and viral mucosal invasion mechanisms. Using this model, our results provide a novel insight into the HSV1 stromal invasion mechanism and for the first time directly demonstrate that HSV1 can penetrate the basement membrane.
format	article
author	Sarah Glorieux Claus Bachert Herman W Favoreel Annelies P Vandekerckhove Lennert Steukers Anamaria Rekecki Wim Van den Broeck Joline Goossens Siska Croubels Reginald F Clayton Hans J Nauwynck
author_facet	Sarah Glorieux Claus Bachert Herman W Favoreel Annelies P Vandekerckhove Lennert Steukers Anamaria Rekecki Wim Van den Broeck Joline Goossens Siska Croubels Reginald F Clayton Hans J Nauwynck
author_sort	Sarah Glorieux
title	Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.
title_short	Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.
title_full	Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.
title_fullStr	Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.
title_full_unstemmed	Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.
title_sort	herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/45c9e670fca84478826f38a952d43c81
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Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa.

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