Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation

As a pollutant, Cd causes severe impact to the environment and damages living organisms. It can be uptaken from the environment by the natural resistance-associated macrophage protein (Nramp) in plants. However, the ion absorption function of Nramp transporter genes in Spirodela polyrhiza has not be...

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Autores principales:	Yan Chen, Gaojie Li, Jingjing Yang, Xuyao Zhao, Zuoliang Sun, Hongwei Hou
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Nramp transporter genes Yeast Subcellular localization Cd accumulation Phytoremediation Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/45c9fcde640346159333b245cecb6a35
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spelling	oai:doaj.org-article:45c9fcde640346159333b245cecb6a352021-11-06T04:16:36ZRole of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation0147-651310.1016/j.ecoenv.2021.112907https://doaj.org/article/45c9fcde640346159333b245cecb6a352021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010198https://doaj.org/toc/0147-6513As a pollutant, Cd causes severe impact to the environment and damages living organisms. It can be uptaken from the environment by the natural resistance-associated macrophage protein (Nramp) in plants. However, the ion absorption function of Nramp transporter genes in Spirodela polyrhiza has not been reported. In this study, SpNramp1, SpNramp2, and SpNramp3 from S. polyrhiza were cloned and their functions were analyzed in S. polyrhiza and yeast. Growth parameters and physicochemical indices of wild-type and transgenic lines were measured under Cd stress. Results revealed that SpNramp1, SpNramp2, and SpNramp3 were identified as plasma membrane-localized transporters, and their roles in transporting Cd were verified in yeast. In S. polyrhiza, SpNramp1 overexpression significantly increased the content of Cd, Fe, Mn, and fresh weight. SpNramp2 overexpression increased Mn and Cd. SpNramp3 overexpression increased Fe and Mn concentrations. These results indicate that SpNramp1, SpNramp2, and SpNramp3 had a different preference for ion absorption. Two S. polyrhiza transgenic lines (OE1 and OE3) were obtained. One of them (OE1) showed a stronger accumulation ability, and the other one (OE3) exhibited tolerance capacity to Cd. This study provides new insight into the functions of SpNramp1, SpNramp2, and SpNramp3 and obtains important enrichment lines (OE1) for manipulating Cd accumulation, phytoremediation, and ecological safety.Yan ChenGaojie LiJingjing YangXuyao ZhaoZuoliang SunHongwei HouElsevierarticleNramp transporter genesYeastSubcellular localizationCd accumulationPhytoremediationEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112907- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nramp transporter genes Yeast Subcellular localization Cd accumulation Phytoremediation Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Nramp transporter genes Yeast Subcellular localization Cd accumulation Phytoremediation Environmental pollution TD172-193.5 Environmental sciences GE1-350 Yan Chen Gaojie Li Jingjing Yang Xuyao Zhao Zuoliang Sun Hongwei Hou Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
description	As a pollutant, Cd causes severe impact to the environment and damages living organisms. It can be uptaken from the environment by the natural resistance-associated macrophage protein (Nramp) in plants. However, the ion absorption function of Nramp transporter genes in Spirodela polyrhiza has not been reported. In this study, SpNramp1, SpNramp2, and SpNramp3 from S. polyrhiza were cloned and their functions were analyzed in S. polyrhiza and yeast. Growth parameters and physicochemical indices of wild-type and transgenic lines were measured under Cd stress. Results revealed that SpNramp1, SpNramp2, and SpNramp3 were identified as plasma membrane-localized transporters, and their roles in transporting Cd were verified in yeast. In S. polyrhiza, SpNramp1 overexpression significantly increased the content of Cd, Fe, Mn, and fresh weight. SpNramp2 overexpression increased Mn and Cd. SpNramp3 overexpression increased Fe and Mn concentrations. These results indicate that SpNramp1, SpNramp2, and SpNramp3 had a different preference for ion absorption. Two S. polyrhiza transgenic lines (OE1 and OE3) were obtained. One of them (OE1) showed a stronger accumulation ability, and the other one (OE3) exhibited tolerance capacity to Cd. This study provides new insight into the functions of SpNramp1, SpNramp2, and SpNramp3 and obtains important enrichment lines (OE1) for manipulating Cd accumulation, phytoremediation, and ecological safety.
format	article
author	Yan Chen Gaojie Li Jingjing Yang Xuyao Zhao Zuoliang Sun Hongwei Hou
author_facet	Yan Chen Gaojie Li Jingjing Yang Xuyao Zhao Zuoliang Sun Hongwei Hou
author_sort	Yan Chen
title	Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
title_short	Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
title_full	Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
title_fullStr	Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
title_full_unstemmed	Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation
title_sort	role of nramp transporter genes of spirodela polyrhiza in cadmium accumulation
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/45c9fcde640346159333b245cecb6a35
work_keys_str_mv	AT yanchen roleofnramptransportergenesofspirodelapolyrhizaincadmiumaccumulation AT gaojieli roleofnramptransportergenesofspirodelapolyrhizaincadmiumaccumulation AT jingjingyang roleofnramptransportergenesofspirodelapolyrhizaincadmiumaccumulation AT xuyaozhao roleofnramptransportergenesofspirodelapolyrhizaincadmiumaccumulation AT zuoliangsun roleofnramptransportergenesofspirodelapolyrhizaincadmiumaccumulation AT hongweihou roleofnramptransportergenesofspirodelapolyrhizaincadmiumaccumulation
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Role of Nramp transporter genes of Spirodela polyrhiza in cadmium accumulation

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