Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study

Abstract Background: Vitrification as the most efficient method of cryopreservation, enables successful storage of oocytes for couples who undergo specific procedures including surgery and chemotherapy. However, the efficacy of in vitro maturation (IVM) methods with vitrified germinal vesicle (GV) o...

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Autores principales:	Razieh Doroudi, Zohre Changizi, Seyed Noureddin Nematollahi-Mahani
Formato:	article
Lenguaje:	EN
Publicado:	Shahid Sadoughi University of Medical Sciences 2021
Materias:	vitrification, germinal vesicle, in vitro oocyte maturation, melatonin, follicular fluid. Gynecology and obstetrics RG1-991 Reproduction QH471-489
Acceso en línea:	https://doaj.org/article/45ce35a08a954246abdb278b075e0c6a
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spelling	oai:doaj.org-article:45ce35a08a954246abdb278b075e0c6a2021-11-16T07:24:40ZEffects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study2476-41082476-377210.18502/ijrm.v19i10.9821https://doaj.org/article/45ce35a08a954246abdb278b075e0c6a2021-10-01T00:00:00Zhttps://doi.org/10.18502/ijrm.v19i10.9821https://doaj.org/toc/2476-4108https://doaj.org/toc/2476-3772Abstract Background: Vitrification as the most efficient method of cryopreservation, enables successful storage of oocytes for couples who undergo specific procedures including surgery and chemotherapy. However, the efficacy of in vitro maturation (IVM) methods with vitrified germinal vesicle (GV) oocytes could be improved. Objective: As melatonin and follicular fluid (FF) might enhance IVM conditions, we used these supplements to assess the maturation rate of vitrified GV oocytes and their artificial fertilization rate. Materials and Methods: Four hundred mouse GV oocytes were harvested, vitrified, and assigned into control (C-Vit-GV) and treatment groups of melatonin (M-Vit-GV), human follicular fluid (HFF-Vit-GV), and a combination (M + HFF-Vit-GV). A non-vitrified group of GV oocytes (non-Vit-GV) and a group of in vivo matured metaphase II (Vivo-MII) oocytes served as control groups to evaluate the vitrification and IVM conditions, respectively. Maturation of GV oocytes to MII and further development to two-cell-stage embryos were determined in the different groups. Results: Development to two-cell embryos was comparable between the Vivo-MII and non-Vit-GV groups. IVM and in vitro fertilization (IVF) results in the non-Vit-GV group were also comparable with the C-Vit-GV oocytes. In addition, the IVM and IVF outcomes were similar across the different treatment groups including the M-Vit-GV, HFF-Vit-GV, M + HFF-Vit-GV, and C-Vit-GV oocytes. Conclusion: Employing an appropriate technique of vitrification followed by suitable IVM conditions can lead to reasonable IVF outcomes which may not benefit from extra supplementations. However, whether utilizing other supplementation formulas could improve the outcome requires further investigation.Razieh DoroudiZohre ChangiziSeyed Noureddin Nematollahi-MahaniShahid Sadoughi University of Medical Sciencesarticlevitrification, germinal vesicle, in vitro oocyte maturation, melatonin, follicular fluid.Gynecology and obstetricsRG1-991ReproductionQH471-489ENInternational Journal of Reproductive BioMedicine, Vol 19, Iss 10, Pp 889-898 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vitrification, germinal vesicle, in vitro oocyte maturation, melatonin, follicular fluid. Gynecology and obstetrics RG1-991 Reproduction QH471-489
spellingShingle	vitrification, germinal vesicle, in vitro oocyte maturation, melatonin, follicular fluid. Gynecology and obstetrics RG1-991 Reproduction QH471-489 Razieh Doroudi Zohre Changizi Seyed Noureddin Nematollahi-Mahani Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
description	Abstract Background: Vitrification as the most efficient method of cryopreservation, enables successful storage of oocytes for couples who undergo specific procedures including surgery and chemotherapy. However, the efficacy of in vitro maturation (IVM) methods with vitrified germinal vesicle (GV) oocytes could be improved. Objective: As melatonin and follicular fluid (FF) might enhance IVM conditions, we used these supplements to assess the maturation rate of vitrified GV oocytes and their artificial fertilization rate. Materials and Methods: Four hundred mouse GV oocytes were harvested, vitrified, and assigned into control (C-Vit-GV) and treatment groups of melatonin (M-Vit-GV), human follicular fluid (HFF-Vit-GV), and a combination (M + HFF-Vit-GV). A non-vitrified group of GV oocytes (non-Vit-GV) and a group of in vivo matured metaphase II (Vivo-MII) oocytes served as control groups to evaluate the vitrification and IVM conditions, respectively. Maturation of GV oocytes to MII and further development to two-cell-stage embryos were determined in the different groups. Results: Development to two-cell embryos was comparable between the Vivo-MII and non-Vit-GV groups. IVM and in vitro fertilization (IVF) results in the non-Vit-GV group were also comparable with the C-Vit-GV oocytes. In addition, the IVM and IVF outcomes were similar across the different treatment groups including the M-Vit-GV, HFF-Vit-GV, M + HFF-Vit-GV, and C-Vit-GV oocytes. Conclusion: Employing an appropriate technique of vitrification followed by suitable IVM conditions can lead to reasonable IVF outcomes which may not benefit from extra supplementations. However, whether utilizing other supplementation formulas could improve the outcome requires further investigation.
format	article
author	Razieh Doroudi Zohre Changizi Seyed Noureddin Nematollahi-Mahani
author_facet	Razieh Doroudi Zohre Changizi Seyed Noureddin Nematollahi-Mahani
author_sort	Razieh Doroudi
title	Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
title_short	Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
title_full	Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
title_fullStr	Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
title_full_unstemmed	Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
title_sort	effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: a laboratory study
publisher	Shahid Sadoughi University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/45ce35a08a954246abdb278b075e0c6a
work_keys_str_mv	AT raziehdoroudi effectsofmelatoninandhumanfollicularfluidsupplementationofinvitromaturationmediumonmousevitrifiedgerminalvesicleoocytesalaboratorystudy AT zohrechangizi effectsofmelatoninandhumanfollicularfluidsupplementationofinvitromaturationmediumonmousevitrifiedgerminalvesicleoocytesalaboratorystudy AT seyednoureddinnematollahimahani effectsofmelatoninandhumanfollicularfluidsupplementationofinvitromaturationmediumonmousevitrifiedgerminalvesicleoocytesalaboratorystudy
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Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study

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