Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline

Salma El-Habashy,1 Hoda Eltaher,1 Ahmed Gaballah,2 Radwa Mehanna,3,4 Amal H El-Kamel1 1Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt; 2Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, 21561, Egypt; 3Medical Ph...

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Autores principales:	El-Habashy S, Eltaher H, Gaballah A, Mehanna R, El-Kamel AH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	bone regeneration bioactivity mesenchymal stem cells drug repositioning osteodifferentiation. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/45ce87441790407da8f77168d17267c1
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spelling	oai:doaj.org-article:45ce87441790407da8f77168d17267c12021-12-02T16:06:11ZBiomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline1178-2013https://doaj.org/article/45ce87441790407da8f77168d17267c12021-02-01T00:00:00Zhttps://www.dovepress.com/biomaterial-based-nanocomposite-for-osteogenic-repurposing-of-doxycycl-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Salma El-Habashy,1 Hoda Eltaher,1 Ahmed Gaballah,2 Radwa Mehanna,3,4 Amal H El-Kamel1 1Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt; 2Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, 21561, Egypt; 3Medical Physiology Department, Faculty of Medicine, Alexandria University, Alexandria, 21131, Egypt; 4Center of Excellence for Research in Regenerative Medicine and Applications CERRMA, Faculty of Medicine, Alexandria University, Alexandria, 21131, EgyptCorrespondence: Amal H El-KamelDepartment of Pharmaceutics, Faculty of Pharmacy, Alexandria University, 1 Khartoum Square, Azarita, PO Box 21521, Alexandria, EgyptTel +20-10-0508 0510Email amalelkamel@yahoo.comBackground: Besides its antimicrobial action, doxycycline (DX) has lately been repurposed as a small-molecule drug for osteogenic purposes. However, osteogenic DX application is impeded by its dose-dependent cytotoxicity. Further, high-dose DX impairs cell differentiation and mineralization.Purpose: Integrating DX into a biomaterial-based delivery system that can control its release would not only ameliorate its cytotoxic actions but also augment its osteogenic activity. In this work, we managed to engineer novel composite DX–hydroxyapatite–polycaprolactone nanoparticles (DX/HAp/PCL) to modify DX osteogenic potential.Methods: Employing a 23-factorial design, we first optimized HApN for surface-area attributes to maximize DX loading. Composite DX/HAp/PCL were then realized using a simple emulsification technique, characterized using various in vitro methods, and evaluated for in vitro osteogenesis.Results: The developed HApN exhibited a favorable crystalline structure, Ca:P elemental ratio (1.67), mesoporous nature, and large surface area. DX/HAp/PCL achieved the highest reported entrapment efficiency (94.77%± 1.23%) of DX in PCL-based particles. The developed composite system achieved controlled release of the water-soluble DX over 24 days. Moreover, the novel composite nanosystem managed to significantly ameliorate DX cytotoxicity on bone-marrow stem cells, as well as enhance its overall proliferation potential. Alkaline phosphatase and mineralization assays revealed superior osteodifferentiation potential of the composite system. Quantification of gene expression demonstrated that while DX solution was able to drive bone-marrow stem cells down the osteogenic lineage into immature osteoblasts after 10-day culture, the innovative composite system allowed maturation of osteodifferentiated cells. To the best of our knowledge, this is the first work to elaborate the impact of DX on the expression of osteogenic genes: RUNX2, OSP, and BSP. Further, the osteogenicity of a DX-loaded particulate-delivery system has not been previously investigated.Conclusion: Our findings indicate that repurposing low-dose DX in complementary biomaterial-based nanosystems can offer a prominent osteogenic candidate for bone-regeneration purposes.Keywords: bone regeneration, bioactivity, mesenchymal stem cells, drug repositioning, osteodifferentiationEl-Habashy SEltaher HGaballah AMehanna REl-Kamel AHDove Medical Pressarticlebone regenerationbioactivitymesenchymal stem cellsdrug repositioningosteodifferentiation.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 1103-1126 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone regeneration bioactivity mesenchymal stem cells drug repositioning osteodifferentiation. Medicine (General) R5-920
spellingShingle	bone regeneration bioactivity mesenchymal stem cells drug repositioning osteodifferentiation. Medicine (General) R5-920 El-Habashy S Eltaher H Gaballah A Mehanna R El-Kamel AH Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline
description	Salma El-Habashy,1 Hoda Eltaher,1 Ahmed Gaballah,2 Radwa Mehanna,3,4 Amal H El-Kamel1 1Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt; 2Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, 21561, Egypt; 3Medical Physiology Department, Faculty of Medicine, Alexandria University, Alexandria, 21131, Egypt; 4Center of Excellence for Research in Regenerative Medicine and Applications CERRMA, Faculty of Medicine, Alexandria University, Alexandria, 21131, EgyptCorrespondence: Amal H El-KamelDepartment of Pharmaceutics, Faculty of Pharmacy, Alexandria University, 1 Khartoum Square, Azarita, PO Box 21521, Alexandria, EgyptTel +20-10-0508 0510Email amalelkamel@yahoo.comBackground: Besides its antimicrobial action, doxycycline (DX) has lately been repurposed as a small-molecule drug for osteogenic purposes. However, osteogenic DX application is impeded by its dose-dependent cytotoxicity. Further, high-dose DX impairs cell differentiation and mineralization.Purpose: Integrating DX into a biomaterial-based delivery system that can control its release would not only ameliorate its cytotoxic actions but also augment its osteogenic activity. In this work, we managed to engineer novel composite DX–hydroxyapatite–polycaprolactone nanoparticles (DX/HAp/PCL) to modify DX osteogenic potential.Methods: Employing a 23-factorial design, we first optimized HApN for surface-area attributes to maximize DX loading. Composite DX/HAp/PCL were then realized using a simple emulsification technique, characterized using various in vitro methods, and evaluated for in vitro osteogenesis.Results: The developed HApN exhibited a favorable crystalline structure, Ca:P elemental ratio (1.67), mesoporous nature, and large surface area. DX/HAp/PCL achieved the highest reported entrapment efficiency (94.77%± 1.23%) of DX in PCL-based particles. The developed composite system achieved controlled release of the water-soluble DX over 24 days. Moreover, the novel composite nanosystem managed to significantly ameliorate DX cytotoxicity on bone-marrow stem cells, as well as enhance its overall proliferation potential. Alkaline phosphatase and mineralization assays revealed superior osteodifferentiation potential of the composite system. Quantification of gene expression demonstrated that while DX solution was able to drive bone-marrow stem cells down the osteogenic lineage into immature osteoblasts after 10-day culture, the innovative composite system allowed maturation of osteodifferentiated cells. To the best of our knowledge, this is the first work to elaborate the impact of DX on the expression of osteogenic genes: RUNX2, OSP, and BSP. Further, the osteogenicity of a DX-loaded particulate-delivery system has not been previously investigated.Conclusion: Our findings indicate that repurposing low-dose DX in complementary biomaterial-based nanosystems can offer a prominent osteogenic candidate for bone-regeneration purposes.Keywords: bone regeneration, bioactivity, mesenchymal stem cells, drug repositioning, osteodifferentiation
format	article
author	El-Habashy S Eltaher H Gaballah A Mehanna R El-Kamel AH
author_facet	El-Habashy S Eltaher H Gaballah A Mehanna R El-Kamel AH
author_sort	El-Habashy S
title	Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline
title_short	Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline
title_full	Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline
title_fullStr	Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline
title_full_unstemmed	Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline
title_sort	biomaterial-based nanocomposite for osteogenic repurposing of doxycycline
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/45ce87441790407da8f77168d17267c1
work_keys_str_mv	AT elhabashys biomaterialbasednanocompositeforosteogenicrepurposingofdoxycycline AT eltaherh biomaterialbasednanocompositeforosteogenicrepurposingofdoxycycline AT gaballaha biomaterialbasednanocompositeforosteogenicrepurposingofdoxycycline AT mehannar biomaterialbasednanocompositeforosteogenicrepurposingofdoxycycline AT elkamelah biomaterialbasednanocompositeforosteogenicrepurposingofdoxycycline
_version_	1718385083795111936

Biomaterial-Based Nanocomposite for Osteogenic Repurposing of Doxycycline

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