Split-cre complementation indicates coincident activity of different genes in vivo.

Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we de...

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Autores principales:	Johannes Hirrlinger, Anja Scheller, Petra G Hirrlinger, Beate Kellert, Wannan Tang, Michael C Wehr, Sandra Goebbels, Andreas Reichenbach, Rolf Sprengel, Moritz J Rossner, Frank Kirchhoff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45cf49cd2e404783b72af995f3e94da0
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spelling	oai:doaj.org-article:45cf49cd2e404783b72af995f3e94da02021-11-25T06:17:37ZSplit-cre complementation indicates coincident activity of different genes in vivo.1932-620310.1371/journal.pone.0004286https://doaj.org/article/45cf49cd2e404783b72af995f3e94da02009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19172189/?tool=EBIhttps://doaj.org/toc/1932-6203Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we designed inactive "split-Cre" fragments that regain Cre activity when overlapping co-expression is controlled by two different promoters. Using transgenic mice and virus-mediated expression of split-Cre, we show that efficient reporter gene activation is achieved in vivo. In the brain of transgenic mice, we genetically defined a subgroup of glial progenitor cells in which the Plp1- and the Gfap-promoter are simultaneously active, giving rise to both astrocytes and NG2-positive glia. Similarly, a subset of interneurons was labelled after viral transfection using Gad67- and Cck1 promoters to express split-Cre. Thus, split-Cre mediated genomic recombination constitutes a powerful spatial and temporal coincidence detector for in vivo targeting.Johannes HirrlingerAnja SchellerPetra G HirrlingerBeate KellertWannan TangMichael C WehrSandra GoebbelsAndreas ReichenbachRolf SprengelMoritz J RossnerFrank KirchhoffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 1, p e4286 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johannes Hirrlinger Anja Scheller Petra G Hirrlinger Beate Kellert Wannan Tang Michael C Wehr Sandra Goebbels Andreas Reichenbach Rolf Sprengel Moritz J Rossner Frank Kirchhoff Split-cre complementation indicates coincident activity of different genes in vivo.
description	Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we designed inactive "split-Cre" fragments that regain Cre activity when overlapping co-expression is controlled by two different promoters. Using transgenic mice and virus-mediated expression of split-Cre, we show that efficient reporter gene activation is achieved in vivo. In the brain of transgenic mice, we genetically defined a subgroup of glial progenitor cells in which the Plp1- and the Gfap-promoter are simultaneously active, giving rise to both astrocytes and NG2-positive glia. Similarly, a subset of interneurons was labelled after viral transfection using Gad67- and Cck1 promoters to express split-Cre. Thus, split-Cre mediated genomic recombination constitutes a powerful spatial and temporal coincidence detector for in vivo targeting.
format	article
author	Johannes Hirrlinger Anja Scheller Petra G Hirrlinger Beate Kellert Wannan Tang Michael C Wehr Sandra Goebbels Andreas Reichenbach Rolf Sprengel Moritz J Rossner Frank Kirchhoff
author_facet	Johannes Hirrlinger Anja Scheller Petra G Hirrlinger Beate Kellert Wannan Tang Michael C Wehr Sandra Goebbels Andreas Reichenbach Rolf Sprengel Moritz J Rossner Frank Kirchhoff
author_sort	Johannes Hirrlinger
title	Split-cre complementation indicates coincident activity of different genes in vivo.
title_short	Split-cre complementation indicates coincident activity of different genes in vivo.
title_full	Split-cre complementation indicates coincident activity of different genes in vivo.
title_fullStr	Split-cre complementation indicates coincident activity of different genes in vivo.
title_full_unstemmed	Split-cre complementation indicates coincident activity of different genes in vivo.
title_sort	split-cre complementation indicates coincident activity of different genes in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/45cf49cd2e404783b72af995f3e94da0
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Split-cre complementation indicates coincident activity of different genes in vivo.

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