A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs

This study evaluates if biomarkers of porcine health status in saliva samples is a mere reflection of serum to detect disease in pigs under field conditions. Four farms from the same commercial company were included to obtain samples from animals with different pathological conditions. A total of 10...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	J. Sánchez, N. Fuentes, F.J. Ibañez-López, I. López-García, A.M. Gutiérrez
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Field study Health status Optimal sample Porcine Salivary analytes Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/45d799aea13a43fc8c4d137de19ff70c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:45d799aea13a43fc8c4d137de19ff70c
record_format	dspace
spelling	oai:doaj.org-article:45d799aea13a43fc8c4d137de19ff70c2021-11-28T04:29:31ZA multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs1751-731110.1016/j.animal.2021.100413https://doaj.org/article/45d799aea13a43fc8c4d137de19ff70c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1751731121002561https://doaj.org/toc/1751-7311This study evaluates if biomarkers of porcine health status in saliva samples is a mere reflection of serum to detect disease in pigs under field conditions. Four farms from the same commercial company were included to obtain samples from animals with different pathological conditions. A total of 10 healthy animals and 10–15 animals from each farm with clinical symptoms of the disease were sampled for paired saliva and blood during a veterinary clinical visit. The biomarker panel included acute-phase proteins (APPs), C-reactive protein (CRP), haptoglobin (Hp), an inflammatory marker, adenosine deaminase (ADA), the total antioxidant capacity (TAC), the levels of essential trace elements, copper (Cu) and zinc (Zn), and the measurement of the total protein content (TP). After detailed statistical analysis, the results showed that saliva could replace serum for APP measurements since a good agreement has been observed between the concentrations of APPs in both body fluids. For any other biomarker, no agreement between the concentrations quantified in serum and saliva samples was observed visually. However, salivary ADA and TP concentrations were statistically significantly higher in the diseased, whereas the statistical tests with serum concentrations were inconclusive. Furthermore, greater differentiation between healthy and diseased animals could be observed when the distribution of biomarkers was analysed in saliva than in other serum samples. The diagnostic power to discriminate between healthy and diseased pigs is similar in saliva and in serum samples. Preliminary regression models may offer an optimal combination of biomarkers for disease detection in saliva (Hp, CRP, and TAC) and serum (Hp, CRP, and Cu), which demands less labour, sample, and financial cost for saliva determinations. The contradictory results observed for TAC, Cu, and Zn levels between body fluids indicate a need for further studies. To sum up, saliva-based biomarkers instead of serum-based biomarkers could contribute to more efficient detection of diseased animals.J. SánchezN. FuentesF.J. Ibañez-LópezI. López-GarcíaA.M. GutiérrezElsevierarticleField studyHealth statusOptimal samplePorcineSalivary analytesAnimal cultureSF1-1100ENAnimal, Vol 15, Iss 12, Pp 100413- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Field study Health status Optimal sample Porcine Salivary analytes Animal culture SF1-1100
spellingShingle	Field study Health status Optimal sample Porcine Salivary analytes Animal culture SF1-1100 J. Sánchez N. Fuentes F.J. Ibañez-López I. López-García A.M. Gutiérrez A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs
description	This study evaluates if biomarkers of porcine health status in saliva samples is a mere reflection of serum to detect disease in pigs under field conditions. Four farms from the same commercial company were included to obtain samples from animals with different pathological conditions. A total of 10 healthy animals and 10–15 animals from each farm with clinical symptoms of the disease were sampled for paired saliva and blood during a veterinary clinical visit. The biomarker panel included acute-phase proteins (APPs), C-reactive protein (CRP), haptoglobin (Hp), an inflammatory marker, adenosine deaminase (ADA), the total antioxidant capacity (TAC), the levels of essential trace elements, copper (Cu) and zinc (Zn), and the measurement of the total protein content (TP). After detailed statistical analysis, the results showed that saliva could replace serum for APP measurements since a good agreement has been observed between the concentrations of APPs in both body fluids. For any other biomarker, no agreement between the concentrations quantified in serum and saliva samples was observed visually. However, salivary ADA and TP concentrations were statistically significantly higher in the diseased, whereas the statistical tests with serum concentrations were inconclusive. Furthermore, greater differentiation between healthy and diseased animals could be observed when the distribution of biomarkers was analysed in saliva than in other serum samples. The diagnostic power to discriminate between healthy and diseased pigs is similar in saliva and in serum samples. Preliminary regression models may offer an optimal combination of biomarkers for disease detection in saliva (Hp, CRP, and TAC) and serum (Hp, CRP, and Cu), which demands less labour, sample, and financial cost for saliva determinations. The contradictory results observed for TAC, Cu, and Zn levels between body fluids indicate a need for further studies. To sum up, saliva-based biomarkers instead of serum-based biomarkers could contribute to more efficient detection of diseased animals.
format	article
author	J. Sánchez N. Fuentes F.J. Ibañez-López I. López-García A.M. Gutiérrez
author_facet	J. Sánchez N. Fuentes F.J. Ibañez-López I. López-García A.M. Gutiérrez
author_sort	J. Sánchez
title	A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs
title_short	A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs
title_full	A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs
title_fullStr	A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs
title_full_unstemmed	A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs
title_sort	multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/45d799aea13a43fc8c4d137de19ff70c
work_keys_str_mv	AT jsanchez amultiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT nfuentes amultiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT fjibanezlopez amultiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT ilopezgarcia amultiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT amgutierrez amultiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT jsanchez multiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT nfuentes multiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT fjibanezlopez multiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT ilopezgarcia multiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs AT amgutierrez multiherdstudyshowsthatsalivaismorethanareflectionofserumbiomarkersinpigs
_version_	1718408374817652736

A multi-herd study shows that saliva is more than a reflection of serum biomarkers in pigs

Ejemplares similares