Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory

Abstract Plant volatile signatures are often used as cues by herbivores to locate their preferred hosts. Here, we report on the volatile organic compounds used by the subterranean root-knot nematode (RKN) Meloidogyne incognita for host location. We compared responses of infective second stage juveni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ruth Kihika, Lucy K. Murungi, Danny Coyne, Margaret Ng’ang’a, Ahmed Hassanali, Peter E. A. Teal, Baldwyn Torto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45d8985f465e4edb833d0d5fa3f37934
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:45d8985f465e4edb833d0d5fa3f37934
record_format	dspace
spelling	oai:doaj.org-article:45d8985f465e4edb833d0d5fa3f379342021-12-02T11:52:23ZParasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory10.1038/s41598-017-02379-82045-2322https://doaj.org/article/45d8985f465e4edb833d0d5fa3f379342017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02379-8https://doaj.org/toc/2045-2322Abstract Plant volatile signatures are often used as cues by herbivores to locate their preferred hosts. Here, we report on the volatile organic compounds used by the subterranean root-knot nematode (RKN) Meloidogyne incognita for host location. We compared responses of infective second stage juveniles (J2s) to root volatiles of three cultivars and one accession of the solanaceous plant, Capsicum annum against moist sand in dual choice assays. J2s were more attracted to the three cultivars than to the accession, relative to controls. GC/MS analysis of the volatiles identified common constituents in each plant, five of which were identified as α-pinene, limonene, 2-methoxy-3-(1-methylpropyl)-pyrazine, methyl salicylate and tridecane. We additionally identified thymol as being specific to the accession. In dose-response assays, a blend of the five components elicited positive chemotaxis (71–88%), whereas individual components elicited varying responses; Methyl salicylate (MeSA) elicited the highest positive chemotaxis (70–80%), α-pinene, limonene and tridecane were intermediate (54–60%), and 2-methoxy-3-(1-methylpropyl)-pyrazine the lowest (49–55%). In contrast, thymol alone or thymol combined with either the preferred natural plant root volatiles or the five-component synthetic blend induced negative chemotaxis. Our results provide insights into RKN-host plant interactions, creating new opportunities for plant breeding programmes towards management of RKNs.Ruth KihikaLucy K. MurungiDanny CoyneMargaret Ng’ang’aAhmed HassanaliPeter E. A. TealBaldwyn TortoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruth Kihika Lucy K. Murungi Danny Coyne Margaret Ng’ang’a Ahmed Hassanali Peter E. A. Teal Baldwyn Torto Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory
description	Abstract Plant volatile signatures are often used as cues by herbivores to locate their preferred hosts. Here, we report on the volatile organic compounds used by the subterranean root-knot nematode (RKN) Meloidogyne incognita for host location. We compared responses of infective second stage juveniles (J2s) to root volatiles of three cultivars and one accession of the solanaceous plant, Capsicum annum against moist sand in dual choice assays. J2s were more attracted to the three cultivars than to the accession, relative to controls. GC/MS analysis of the volatiles identified common constituents in each plant, five of which were identified as α-pinene, limonene, 2-methoxy-3-(1-methylpropyl)-pyrazine, methyl salicylate and tridecane. We additionally identified thymol as being specific to the accession. In dose-response assays, a blend of the five components elicited positive chemotaxis (71–88%), whereas individual components elicited varying responses; Methyl salicylate (MeSA) elicited the highest positive chemotaxis (70–80%), α-pinene, limonene and tridecane were intermediate (54–60%), and 2-methoxy-3-(1-methylpropyl)-pyrazine the lowest (49–55%). In contrast, thymol alone or thymol combined with either the preferred natural plant root volatiles or the five-component synthetic blend induced negative chemotaxis. Our results provide insights into RKN-host plant interactions, creating new opportunities for plant breeding programmes towards management of RKNs.
format	article
author	Ruth Kihika Lucy K. Murungi Danny Coyne Margaret Ng’ang’a Ahmed Hassanali Peter E. A. Teal Baldwyn Torto
author_facet	Ruth Kihika Lucy K. Murungi Danny Coyne Margaret Ng’ang’a Ahmed Hassanali Peter E. A. Teal Baldwyn Torto
author_sort	Ruth Kihika
title	Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory
title_short	Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory
title_full	Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory
title_fullStr	Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory
title_full_unstemmed	Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory
title_sort	parasitic nematode meloidogyne incognita interactions with different capsicum annum cultivars reveal the chemical constituents modulating root herbivory
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/45d8985f465e4edb833d0d5fa3f37934
work_keys_str_mv	AT ruthkihika parasiticnematodemeloidogyneincognitainteractionswithdifferentcapsicumannumcultivarsrevealthechemicalconstituentsmodulatingrootherbivory AT lucykmurungi parasiticnematodemeloidogyneincognitainteractionswithdifferentcapsicumannumcultivarsrevealthechemicalconstituentsmodulatingrootherbivory AT dannycoyne parasiticnematodemeloidogyneincognitainteractionswithdifferentcapsicumannumcultivarsrevealthechemicalconstituentsmodulatingrootherbivory AT margaretnganga parasiticnematodemeloidogyneincognitainteractionswithdifferentcapsicumannumcultivarsrevealthechemicalconstituentsmodulatingrootherbivory AT ahmedhassanali parasiticnematodemeloidogyneincognitainteractionswithdifferentcapsicumannumcultivarsrevealthechemicalconstituentsmodulatingrootherbivory AT petereateal parasiticnematodemeloidogyneincognitainteractionswithdifferentcapsicumannumcultivarsrevealthechemicalconstituentsmodulatingrootherbivory AT baldwyntorto parasiticnematodemeloidogyneincognitainteractionswithdifferentcapsicumannumcultivarsrevealthechemicalconstituentsmodulatingrootherbivory
_version_	1718395043677470720

Parasitic nematode Meloidogyne incognita interactions with different Capsicum annum cultivars reveal the chemical constituents modulating root herbivory

Ejemplares similares