Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.

In anti-cancer therapy mediated by a nanoparticle-based drug delivery system (DDS), overall efficacy depends on the release efficiency of cargos from the nanoparticles in the cancer cells as well as the specificity of delivery to tumor tissue. However, conventional liposome-based DDS have no mechani...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shoko Itakura, Susumu Hama, Takashi Ohgita, Kentaro Kogure
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45e89a5741284afbb539574829cd8f9b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:45e89a5741284afbb539574829cd8f9b
record_format	dspace
spelling	oai:doaj.org-article:45e89a5741284afbb539574829cd8f9b2021-11-25T05:55:22ZDevelopment of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.1932-620310.1371/journal.pone.0111181https://doaj.org/article/45e89a5741284afbb539574829cd8f9b2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0111181https://doaj.org/toc/1932-6203In anti-cancer therapy mediated by a nanoparticle-based drug delivery system (DDS), overall efficacy depends on the release efficiency of cargos from the nanoparticles in the cancer cells as well as the specificity of delivery to tumor tissue. However, conventional liposome-based DDS have no mechanism for specifically releasing the encapsulated cargos inside the cancer cells. To overcome this barrier, we developed nanoparticles containing a novel liposomal membrane destabilization peptide (LMDP) that can destabilize membranes by cleavage with intramembranous proteases on/in cancer cells. Calcein encapsulated in liposomes modified with LMDP (LMDP-lipo) was effectively released in the presence of a membrane fraction containing an LMDP-cleavable protease. The release was inhibited by a protease inhibitor, suggesting that LMDP-lipo could effectively release its cargo into cells in response to a cancer-specific protease. Moreover, when LMDP-lipo contained fusogenic lipids, the release of cargo was accelerated, suggesting that the fusion of LMDP-lipo with cellular membranes was the initial step in the intracellular delivery. Time-lapse microscopic observations showed that the release of cargo from LMDP-lipo occurred immediately after association of LMDP-lipo with target cells. Consequently, LMDP-lipo could be a useful nanoparticle capable of effective release of cargos specifically into targeted cancer cells.Shoko ItakuraSusumu HamaTakashi OhgitaKentaro KogurePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e111181 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shoko Itakura Susumu Hama Takashi Ohgita Kentaro Kogure Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.
description	In anti-cancer therapy mediated by a nanoparticle-based drug delivery system (DDS), overall efficacy depends on the release efficiency of cargos from the nanoparticles in the cancer cells as well as the specificity of delivery to tumor tissue. However, conventional liposome-based DDS have no mechanism for specifically releasing the encapsulated cargos inside the cancer cells. To overcome this barrier, we developed nanoparticles containing a novel liposomal membrane destabilization peptide (LMDP) that can destabilize membranes by cleavage with intramembranous proteases on/in cancer cells. Calcein encapsulated in liposomes modified with LMDP (LMDP-lipo) was effectively released in the presence of a membrane fraction containing an LMDP-cleavable protease. The release was inhibited by a protease inhibitor, suggesting that LMDP-lipo could effectively release its cargo into cells in response to a cancer-specific protease. Moreover, when LMDP-lipo contained fusogenic lipids, the release of cargo was accelerated, suggesting that the fusion of LMDP-lipo with cellular membranes was the initial step in the intracellular delivery. Time-lapse microscopic observations showed that the release of cargo from LMDP-lipo occurred immediately after association of LMDP-lipo with target cells. Consequently, LMDP-lipo could be a useful nanoparticle capable of effective release of cargos specifically into targeted cancer cells.
format	article
author	Shoko Itakura Susumu Hama Takashi Ohgita Kentaro Kogure
author_facet	Shoko Itakura Susumu Hama Takashi Ohgita Kentaro Kogure
author_sort	Shoko Itakura
title	Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.
title_short	Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.
title_full	Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.
title_fullStr	Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.
title_full_unstemmed	Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.
title_sort	development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/45e89a5741284afbb539574829cd8f9b
work_keys_str_mv	AT shokoitakura developmentofnanoparticlesincorporatinganovelliposomalmembranedestabilizationpeptideforefficientreleaseofcargosintocancercells AT susumuhama developmentofnanoparticlesincorporatinganovelliposomalmembranedestabilizationpeptideforefficientreleaseofcargosintocancercells AT takashiohgita developmentofnanoparticlesincorporatinganovelliposomalmembranedestabilizationpeptideforefficientreleaseofcargosintocancercells AT kentarokogure developmentofnanoparticlesincorporatinganovelliposomalmembranedestabilizationpeptideforefficientreleaseofcargosintocancercells
_version_	1718414395699101696

Development of nanoparticles incorporating a novel liposomal membrane destabilization peptide for efficient release of cargos into cancer cells.

Ejemplares similares