Systematic review and meta-analysis for the biotechnological production of THC in Morocco

Cannabinoids have promising therapeutic potential. Δ9-tetrahydrocannabinol (THC), the most important psychotropic active ingredient of Cannabis Sativa L, has been the subject of several chemical, pharmacological and biosynthetic studies. In this context, a meta-analysis of biotechnological processes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	badrana Fadwa, Mokhtari Abdelrhani, Safini Najete, Soulaymani Abdelmajid, El fahime Elmostafa, El Amri Hamid
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/45ed30f6a55e49fd819aee10d8060971
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:45ed30f6a55e49fd819aee10d8060971
record_format	dspace
spelling	oai:doaj.org-article:45ed30f6a55e49fd819aee10d80609712021-12-02T17:11:26ZSystematic review and meta-analysis for the biotechnological production of THC in Morocco2267-124210.1051/e3sconf/202131902012https://doaj.org/article/45ed30f6a55e49fd819aee10d80609712021-01-01T00:00:00Zhttps://www.e3s-conferences.org/articles/e3sconf/pdf/2021/95/e3sconf_vigisan_02012.pdfhttps://doaj.org/toc/2267-1242Cannabinoids have promising therapeutic potential. Δ9-tetrahydrocannabinol (THC), the most important psychotropic active ingredient of Cannabis Sativa L, has been the subject of several chemical, pharmacological and biosynthetic studies. In this context, a meta-analysis of biotechnological processes applied to the production of recombinant cannabinoid THC worldwide was carried out. The objective was to highlight the potential of these processes on the Moroccan variety of Cannabis Sativa. The PubMed, ScienceDirect and Web of Science search motors were used to search for original scientific work presenting biotechnological tools used in the production of THC. The scientific articles exploited are those published before the end of 2020. Succinct analysis of the experimental work performed showed that expression of the gene encoding Cannabis Sativa L. THCA synthase was performed on prokaryotic and eukaryotic expression systems. Currently, no functional expression could be obtained in E. coli. Whereas, production of recombinant protein (THCA Synthase) associated with significant enzymatic activity was obtained in P. pastoris cultures (F. Saccharomycetaceae). The exploitation of the sequences showed the presence of a large similarity between the THCA Synthase gene of cannabis Sativa of the Moroccan variety and the mRNA precursor of the same gene reported in several studies. This will allow us to use a specific signal sequence of choice for an adopted expression host, in order to produce the recombinant THCA synthase enzyme from the Moroccan Cannabis Sativa L strain on P. pastoris cultures.badrana FadwaMokhtari AbdelrhaniSafini NajeteSoulaymani AbdelmajidEl fahime ElmostafaEl Amri HamidEDP SciencesarticleEnvironmental sciencesGE1-350ENFRE3S Web of Conferences, Vol 319, p 02012 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Environmental sciences GE1-350
spellingShingle	Environmental sciences GE1-350 badrana Fadwa Mokhtari Abdelrhani Safini Najete Soulaymani Abdelmajid El fahime Elmostafa El Amri Hamid Systematic review and meta-analysis for the biotechnological production of THC in Morocco
description	Cannabinoids have promising therapeutic potential. Δ9-tetrahydrocannabinol (THC), the most important psychotropic active ingredient of Cannabis Sativa L, has been the subject of several chemical, pharmacological and biosynthetic studies. In this context, a meta-analysis of biotechnological processes applied to the production of recombinant cannabinoid THC worldwide was carried out. The objective was to highlight the potential of these processes on the Moroccan variety of Cannabis Sativa. The PubMed, ScienceDirect and Web of Science search motors were used to search for original scientific work presenting biotechnological tools used in the production of THC. The scientific articles exploited are those published before the end of 2020. Succinct analysis of the experimental work performed showed that expression of the gene encoding Cannabis Sativa L. THCA synthase was performed on prokaryotic and eukaryotic expression systems. Currently, no functional expression could be obtained in E. coli. Whereas, production of recombinant protein (THCA Synthase) associated with significant enzymatic activity was obtained in P. pastoris cultures (F. Saccharomycetaceae). The exploitation of the sequences showed the presence of a large similarity between the THCA Synthase gene of cannabis Sativa of the Moroccan variety and the mRNA precursor of the same gene reported in several studies. This will allow us to use a specific signal sequence of choice for an adopted expression host, in order to produce the recombinant THCA synthase enzyme from the Moroccan Cannabis Sativa L strain on P. pastoris cultures.
format	article
author	badrana Fadwa Mokhtari Abdelrhani Safini Najete Soulaymani Abdelmajid El fahime Elmostafa El Amri Hamid
author_facet	badrana Fadwa Mokhtari Abdelrhani Safini Najete Soulaymani Abdelmajid El fahime Elmostafa El Amri Hamid
author_sort	badrana Fadwa
title	Systematic review and meta-analysis for the biotechnological production of THC in Morocco
title_short	Systematic review and meta-analysis for the biotechnological production of THC in Morocco
title_full	Systematic review and meta-analysis for the biotechnological production of THC in Morocco
title_fullStr	Systematic review and meta-analysis for the biotechnological production of THC in Morocco
title_full_unstemmed	Systematic review and meta-analysis for the biotechnological production of THC in Morocco
title_sort	systematic review and meta-analysis for the biotechnological production of thc in morocco
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/45ed30f6a55e49fd819aee10d8060971
work_keys_str_mv	AT badranafadwa systematicreviewandmetaanalysisforthebiotechnologicalproductionofthcinmorocco AT mokhtariabdelrhani systematicreviewandmetaanalysisforthebiotechnologicalproductionofthcinmorocco AT safininajete systematicreviewandmetaanalysisforthebiotechnologicalproductionofthcinmorocco AT soulaymaniabdelmajid systematicreviewandmetaanalysisforthebiotechnologicalproductionofthcinmorocco AT elfahimeelmostafa systematicreviewandmetaanalysisforthebiotechnologicalproductionofthcinmorocco AT elamrihamid systematicreviewandmetaanalysisforthebiotechnologicalproductionofthcinmorocco
_version_	1718381494748053504

Systematic review and meta-analysis for the biotechnological production of THC in Morocco

Ejemplares similares