Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes

Abstract The continued success of malaria control efforts requires the development, study and implementation of new technologies that circumvent insecticide resistance. We previously demonstrated that fungal pathogens can provide an effective delivery system for mosquitocidal or malariacidal biomole...

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Autores principales:	Etienne Bilgo, Brian Lovett, Weiguo Fang, Niraj Bende, Glenn F. King, Abdoulaye Diabate, Raymond J. St. Leger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/45f06e8bf9dc4ef391e4686cc0834d05
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spelling	oai:doaj.org-article:45f06e8bf9dc4ef391e4686cc0834d052021-12-02T11:52:41ZImproved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes10.1038/s41598-017-03399-02045-2322https://doaj.org/article/45f06e8bf9dc4ef391e4686cc0834d052017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03399-0https://doaj.org/toc/2045-2322Abstract The continued success of malaria control efforts requires the development, study and implementation of new technologies that circumvent insecticide resistance. We previously demonstrated that fungal pathogens can provide an effective delivery system for mosquitocidal or malariacidal biomolecules. Here we compared genes from arthropod predators encoding insect specific sodium, potassium and calcium channel blockers for their ability to improve the efficacy of Metarhizium against wild-caught, insecticide-resistant anophelines. Toxins expressed under control of a hemolymph-specific promoter increased fungal lethality to mosquitoes at spore dosages as low as one conidium per mosquito. One of the most potent, the EPA approved Hybrid (Ca++/K+ channel blocker), was studied for pre-lethal effects. These included reduced blood feeding behavior, with almost 100% of insects infected with ~6 spores unable to transmit malaria within 5 days post-infection, surpassing the World Health Organization threshold for successful vector control agents. Furthermore, recombinant strains co-expressing Hybrid toxin and AaIT (Na+ channel blocker) produced synergistic effects, requiring 45% fewer spores to kill half of the mosquitoes in 5 days as single toxin strains. Our results identify a repertoire of toxins with different modes of action that improve the utility of entomopathogens as a technology that is compatible with existing insecticide-based control methods.Etienne BilgoBrian LovettWeiguo FangNiraj BendeGlenn F. KingAbdoulaye DiabateRaymond J. St. LegerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Etienne Bilgo Brian Lovett Weiguo Fang Niraj Bende Glenn F. King Abdoulaye Diabate Raymond J. St. Leger Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
description	Abstract The continued success of malaria control efforts requires the development, study and implementation of new technologies that circumvent insecticide resistance. We previously demonstrated that fungal pathogens can provide an effective delivery system for mosquitocidal or malariacidal biomolecules. Here we compared genes from arthropod predators encoding insect specific sodium, potassium and calcium channel blockers for their ability to improve the efficacy of Metarhizium against wild-caught, insecticide-resistant anophelines. Toxins expressed under control of a hemolymph-specific promoter increased fungal lethality to mosquitoes at spore dosages as low as one conidium per mosquito. One of the most potent, the EPA approved Hybrid (Ca++/K+ channel blocker), was studied for pre-lethal effects. These included reduced blood feeding behavior, with almost 100% of insects infected with ~6 spores unable to transmit malaria within 5 days post-infection, surpassing the World Health Organization threshold for successful vector control agents. Furthermore, recombinant strains co-expressing Hybrid toxin and AaIT (Na+ channel blocker) produced synergistic effects, requiring 45% fewer spores to kill half of the mosquitoes in 5 days as single toxin strains. Our results identify a repertoire of toxins with different modes of action that improve the utility of entomopathogens as a technology that is compatible with existing insecticide-based control methods.
format	article
author	Etienne Bilgo Brian Lovett Weiguo Fang Niraj Bende Glenn F. King Abdoulaye Diabate Raymond J. St. Leger
author_facet	Etienne Bilgo Brian Lovett Weiguo Fang Niraj Bende Glenn F. King Abdoulaye Diabate Raymond J. St. Leger
author_sort	Etienne Bilgo
title	Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
title_short	Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
title_full	Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
title_fullStr	Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
title_full_unstemmed	Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
title_sort	improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/45f06e8bf9dc4ef391e4686cc0834d05
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Improved efficacy of an arthropod toxin expressing fungus against insecticide-resistant malaria-vector mosquitoes

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