Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

Curcumin (CUR) has interesting properties to cure cancer. Cold atmospheric plasma (CAP) is also an emerging biomedical technique that has great potential for cancer treatment. Therefore, the combined effect of CAP and CUR on inducing cytotoxicity and apoptosis of melanoma cancer cells might be promi...

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Autores principales:	Zahra Yazdani, Pooyan Mehrabanjoubani, Alireza Rafiei, Pourya Biparva, Mostafa Kardan
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/45fb38f5c13a4c688a66c32871c867eb
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spelling	oai:doaj.org-article:45fb38f5c13a4c688a66c32871c867eb2021-11-29T00:56:08ZCombined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer2314-614110.1155/2021/1969863https://doaj.org/article/45fb38f5c13a4c688a66c32871c867eb2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/1969863https://doaj.org/toc/2314-6141Curcumin (CUR) has interesting properties to cure cancer. Cold atmospheric plasma (CAP) is also an emerging biomedical technique that has great potential for cancer treatment. Therefore, the combined effect of CAP and CUR on inducing cytotoxicity and apoptosis of melanoma cancer cells might be promising. Here, we investigated the combined effects of CAP and CUR on cytotoxicity and apoptosis in B16-F10 melanoma cancer cells compared to L929 normal cells using MTT method, acridine orange/ethidium bromide fluorescence microscopic assay, and Annexin V/PI flow cytometry. In addition, the activation of apoptosis pathways was evaluated using BCL2, BAX, and Caspase-3 (CASP3) gene expression and ratio of BAX to BCL2 (BAX/BCL2). Finally, in silico study was performed to suggest the molecular mechanism of this combination therapy on melanoma cancer. Results showed that although combination therapy with CUR and CAP has cytotoxic and apoptotic effects on cancer cells, it did not improve apoptosis rate in melanoma B16-F10 cancer cells compared to monotherapy with CAP or CUR. In addition, evaluation of gene expression in cancer cell line confirmed that CUR and CAP concomitant treatment did not enhance the expression of apoptotic genes. In silico analysis of docked model suggested that CUR blocks aquaporin- (AQP-) 1 channel and prevents penetration of CAP-induced ROS into the cells. In conclusion, combination therapy with CAP and CUR does not improve the anticancer effect of each alone.Zahra YazdaniPooyan MehrabanjoubaniAlireza RafieiPourya BiparvaMostafa KardanHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Zahra Yazdani Pooyan Mehrabanjoubani Alireza Rafiei Pourya Biparva Mostafa Kardan Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer
description	Curcumin (CUR) has interesting properties to cure cancer. Cold atmospheric plasma (CAP) is also an emerging biomedical technique that has great potential for cancer treatment. Therefore, the combined effect of CAP and CUR on inducing cytotoxicity and apoptosis of melanoma cancer cells might be promising. Here, we investigated the combined effects of CAP and CUR on cytotoxicity and apoptosis in B16-F10 melanoma cancer cells compared to L929 normal cells using MTT method, acridine orange/ethidium bromide fluorescence microscopic assay, and Annexin V/PI flow cytometry. In addition, the activation of apoptosis pathways was evaluated using BCL2, BAX, and Caspase-3 (CASP3) gene expression and ratio of BAX to BCL2 (BAX/BCL2). Finally, in silico study was performed to suggest the molecular mechanism of this combination therapy on melanoma cancer. Results showed that although combination therapy with CUR and CAP has cytotoxic and apoptotic effects on cancer cells, it did not improve apoptosis rate in melanoma B16-F10 cancer cells compared to monotherapy with CAP or CUR. In addition, evaluation of gene expression in cancer cell line confirmed that CUR and CAP concomitant treatment did not enhance the expression of apoptotic genes. In silico analysis of docked model suggested that CUR blocks aquaporin- (AQP-) 1 channel and prevents penetration of CAP-induced ROS into the cells. In conclusion, combination therapy with CAP and CUR does not improve the anticancer effect of each alone.
format	article
author	Zahra Yazdani Pooyan Mehrabanjoubani Alireza Rafiei Pourya Biparva Mostafa Kardan
author_facet	Zahra Yazdani Pooyan Mehrabanjoubani Alireza Rafiei Pourya Biparva Mostafa Kardan
author_sort	Zahra Yazdani
title	Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer
title_short	Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer
title_full	Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer
title_fullStr	Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer
title_full_unstemmed	Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer
title_sort	combined effect of cold atmospheric plasma and curcumin in melanoma cancer
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/45fb38f5c13a4c688a66c32871c867eb
work_keys_str_mv	AT zahrayazdani combinedeffectofcoldatmosphericplasmaandcurcumininmelanomacancer AT pooyanmehrabanjoubani combinedeffectofcoldatmosphericplasmaandcurcumininmelanomacancer AT alirezarafiei combinedeffectofcoldatmosphericplasmaandcurcumininmelanomacancer AT pouryabiparva combinedeffectofcoldatmosphericplasmaandcurcumininmelanomacancer AT mostafakardan combinedeffectofcoldatmosphericplasmaandcurcumininmelanomacancer
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Combined Effect of Cold Atmospheric Plasma and Curcumin in Melanoma Cancer

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